An analysis of age-related changes in the structure, cell dynamics and neuroepithelial regeneration of olfactory mucosa

• Project/Area Number: 18799002
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: The University of Tokyo
• Principal Investigator: KONDO Kenji The University of Tokyo, 医学部附属病院, 講師 (40334370)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 嗅神経細胞 / 加齢変化 / 再生 / 老化 / 免疫組織化学

Research Abstract

マウス嗅上皮における変性部位の割合は加齢に伴って増加し、また高度変性部位の割合も増加した。また上皮下のBowman腺の形態異常も加齢に従って増加し、特に嗅上皮の変性部位の上皮下に高頻度で認められた。生理的加齢に伴って基底細胞の増殖と嗅上皮のアポトーシスの頻度はともに減少したが、前駆細胞から成熟嗅神経細胞に分化するまでの時間経過はほぼ一定であった。さらに嗅上皮傷害モデルマウスの解析では嗅上皮の再生能力が加齢変化とともに低下し、これは細胞の分化過程の遅延ではなく前駆細胞の増殖能の低下による可能性が高いと考えられた。

Research Products

• [Journal Article] Distribution and severity of spontaneous lesions in the neuroepithelium and Bowman's glands in mouse olfactory mucosa : age-related progression (2009)
  • Author(s): Kondo K, Watanabe K, Sakamoto T, Suzukawa K, Nibu K, Kaga K, Yamasoba T
  • Journal Title: Cell Tissue Res 335 Pages: 489-503
  • Peer Reviewed
• [Journal Article] Distribution and severity of spontaneous lesions in the neuroepithelium and Bowman's glands in mouse olfactory mucosa : age-related progression (2009)
  • Author(s): Kondo K, Watanabe K, S akamoto T, et.al.
  • Journal Title: Cell Tissue Res 355 Pages: 489-503
  • Peer Reviewed
• [Journal Article] Modified nasal specula and flexible holder for endoscopic nasal surgery (2008)
  • Author(s): Ushio M, Nakaya M, Kondo K, Suzuki M, Yamasoba T.
  • Journal Title: Laryngoscope 118 Pages: 1293-4
  • Peer Reviewed
• [Journal Article] Immunohistochemical study of m1-m5 muscarinic receptors and nNOS in human inferior turbinate mucosa (2007)
  • Author(s): Nakaya M. Kondo K, Kaga K
  • Journal Title: Acta Otolaryngol Suppl 559 Pages: 103-7
  • Peer Reviewed
• [Journal Article] Age-related change of the axonal diameter of the olfactory nerve in mouse lamina propria (2007)
  • Author(s): Watanabe K, Kondo K, Yamasoba T, Kaga K
  • Journal Title: Acta Otolaryngol Suppl 559 Pages: 108-112
  • Peer Reviewed
• [Journal Article] Musashi-1 expression in the postnatal mouse olfactory epithelium (2007)
  • Author(s): Watanabe K, Kondo K, Takeuchi N, Okano H, Yamasoba T
  • Journal Title: Neuroreport 18 Pages: 641-644
  • Peer Reviewed
• [Journal Article] Methimazole-induced cell death in rat olfactory receptor neurons occurs via apoptosis triggered through mitochondrial cytochrome c-mediated caspase-3 activation pathway (2007)
  • Author(s): Sakamoto T, Kondo K, Kashio A, Suzukawa K, Yamasoba K
  • Journal Title: J Neurosci Res 85 Pages: 548-557
  • Peer Reviewed
• [Journal Article] Immunohistochemical study of m1-m5 muscarinic receptors and nNos in human inferior turbinate mucosa (2007)
  • Author(s): Nakaya M, Kondo K, Kaga K
  • Journal Title: Acta Otolaryngol supplemet559 Pages: 103-107
  • Peer Reviewed
• [Journal Article] Age-related change of the axonal diameter of the olfactory nerve in mouse lamina propria (2007)
  • Author(s): Watanabe K, Kondo K, Yamasoba T, Kaga K
  • Journal Title: Acta Otolaryngol supplemet559 Pages: 108-112
  • Peer Reviewed
• [Journal Article] Methimazole-induced cell death in rat olfactory receptor neurons occurs via apoptosis triggered through mitochondrial cytochrome c-mediated caspase-3 activation pathway (2007)
  • Author(s): Sakamoto T, Kondo K, Kashio A, Suzukawa K, Yamasoba T
  • Journal Title: Journal of Neuroscience Research 85 Pages: 548-557
• [Journal Article] Age-related changes in cell density and the proliferation rate of olfactory ensheathing cells in the lamina propria of postnatal mouse olfactory mucosa (2006)
  • Author(s): Watanabe K, Kondo K, Takeuchi N, Nibu K, Kaga K.
  • Journal Title: Brain Res 1116 Pages: 82-92
  • Peer Reviewed
• [Journal Article] Supporting cell proliferation after hair cell injury in mature guinea pig cochlea in vivo (2006)
  • Author(s): Yamasoba T, Kondo K
  • Journal Title: Cell Tissue Res 325 Pages: 23-31
  • Peer Reviewed
• [Journal Article] Age-related changes in cell density and the proliferation rate of olfactory ensheathing cells in the lamina propria of postnatal mouse olfactory mucosa (2006)
  • Author(s): Watanabe K, Kondo K, Takeuchi N, Nibu K, Kaga K
  • Journal Title: Brain Research 1116 Pages: 82-92
• [Presentation] 加齢による嗅覚障害の特徴 (2008)
  • Author(s): 近藤健二
  • Organizer: 感覚器シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-03-07
• [Presentation] 加齢マウスの嗅上皮変性部位の上皮下にみられるBowman腺の変化 (2008)
  • Author(s): 近藤健二、渡辺健太、坂本幸士、鈴川圭吾、山岨達也
  • Organizer: 第109回日本耳鼻咽喉科学会
  • Place of Presentation: 大阪
• [Presentation] 加齢マウスの嗅上皮変性部位の上皮下にみられるBowman腺の変化 (2008)
  • Author(s): 近藤健二、渡辺健太、坂本幸士、鈴川佳吾、丹生健一、山岨達也
  • Organizer: 日本味と匂い学会
  • Place of Presentation: 富山
• [Presentation] 嗅粘膜における性ホルモン受容体の発現と分布に関する免疫組織学的検討 (2008)
  • Author(s): 近藤健二、鈴川佳吾、金谷佳織、坂本幸士、渡辺健太、山岨達也
  • Organizer: 第47回日本鼻科学会
  • Place of Presentation: 名古屋
• [Presentation] 加齢マウスの嗅上皮変性部位の上皮下にみられるBowman腺の変化 (2008)
  • Author(s): 近藤健二・渡辺健太・坂本幸士・鈴川佳吾・山岨達也
  • Organizer: 日本耳鼻咽喉科学会学術講演会
  • Place of Presentation: 大阪
• [Presentation] 加齢マウスの嗅上皮変性部位の上皮下にみられるBowman腺の変化 (2008)
  • Author(s): 近藤健二・渡辺健太・坂本幸士・鈴川佳吾・丹生健一・山岨達也
  • Organizer: 日本味と匂学会
  • Place of Presentation: 富山
• [Presentation] 嗅粘膜における性ホルモン受容体の発現と分布に関する免疫組織学的検討 (2008)
  • Author(s): 近藤健二・鈴川佳吾・金谷佳織・坂本幸士・渡辺健太・山岨達也
  • Organizer: 日本鼻科学会
  • Place of Presentation: 名古屋
• [Presentation] 生理的加齢変化により進行するマウス嗅上皮の変性 (2007)
  • Author(s): 近藤健二、渡辺健太、丹生健一、山岨達也
  • Organizer: 第108回日本耳鼻咽喉科学会
  • Place of Presentation: 金沢
• [Presentation] 加齢マウスの嗅上皮変性部位の上皮下に見られるBowman腺の変化 (2007)
  • Author(s): 近藤健二、渡辺健太、鈴川佳吾、山岨達也
  • Organizer: 第46回日本鼻科学会
  • Place of Presentation: 宇都宮
• [Presentation] マウス嗅上皮の基底細胞から成熟嗅神経細胞への分化過程及び分化速度の解析:生理的加齢変化における検討 (2006)
  • Author(s): 近藤健二、丹生健一、竹内直信
  • Organizer: 第45回日本鼻科学会
  • Place of Presentation: 三重
• [Book] 嗅覚とアンチエイジング.アンチエイジング医学の基礎と臨床 (2008)
  • Author(s): 近藤健二
  • Publisher: MEDICALVIEW