Analysis of interaction between botulinum neurotoxin type D and Phosphatidylethanolamine

• Project/Area Number: 18799011
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Fujita Health University
• Principal Investigator: TSUKAMOTO Kentaro Fujita Health University, 医学部, 助教 (80434596)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,470,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: Clostridium botulinum / ボツリヌス毒素 / 受容体 / ガングリオシド / ホスファチジルエタノールアミン / 表面プラズモン共鳴 / 細菌 / 脂質 / リボソーム / 細菌毒素 / ボツリヌス菌 / 神経毒素 / ホスファチアジルエタノールアミン

Research Abstract

ボツリヌス神経毒素(A〜G型)は型特異的な受容体に結合する。そのうち、ボツリヌスD型毒素は他の型とは異なり、グリセロリン脂質の一つPEに結合する。本研究では、ボツリヌスD型神経毒素とPEの分子間相互作用について調べ、毒素分子内のPE認識に関わるアミノ酸残基を特定した。さらに表面プラズモン共鳴による結合解析の実験系を構築し、蛋白質-脂質の分子間相互作用について定量的に解析することを可能にした。

Research Products

• [Journal Article] Identification of the receptor-binding sites in the carboxyl-terminal half of the heavy chain of botulinum neurotoxin types C and D (2008)
  • Author(s): Tsukamoto, K., Kozai, Y., Ihara, H., Kohda, T., Mukamoto, M., Tsuji, T. and Kozaki, S.
  • Journal Title: Microb Pathog 44 Pages: 484-493
  • Peer Reviewed
• [Journal Article] A nasal vaccine comprising B-subunit derivative of Shiga toxin 2 for cross-protection against Shiga toxin types 1 and 2 (2008)
  • Author(s): Tsuji, T., Shimizu, T., Sasaki, K., Tsukamoto, K., Arimitsu, H., Ochi, S., Taniguchi, K., Noda, M., Neri, P. and Mori, H.
  • Journal Title: Vaccine 26 Pages: 2092-2099
  • Peer Reviewed
• [Journal Article] Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin (2008)
  • Author(s): Tsuji, T., Shimizu, T., Sasaki, K., Shimizu, Y., Tsukamoto, K., Arimitsu, H., Ochi, S., Sugiyama, S., Taniguchi, K., Neri, P. and Mori, H.
  • Journal Title: Vaccine 26 Pages: 469-476
  • Peer Reviewed
• [Journal Article] Molecular properties of each subcomponent in Clostridium botulinum type B haemagglutinin complex (2008)
  • Author(s): Arimitsu, H., Sakaguchi, Y., Lee, J. C., Ochi, S., Tsukamoto, K., Yamamoto, Y., Ma, S., Tsuji, T. and Oguma, K.
  • Journal Title: Microb Pathog 45 Pages: 142-149
  • Peer Reviewed
• [Journal Article] Molecular properties of each subcomponent in Clostridium botulinum type B haemagglutinin complex (2008)
  • Author(s): Arimitsu, H., et.al.
  • Journal Title: Microbial Pathogenesis 45 Pages: 142-149
  • Peer Reviewed
• [Journal Article] Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin (2008)
  • Author(s): Tsuji, T. et. al.
  • Journal Title: Vaccine 26 Pages: 469-476
  • Peer Reviewed
• [Journal Article] Identification of the receptor-binding sites in the carboxyl-terminal half of the heavy chain of botulinum neurotoxin types C and D (2008)
  • Author(s): Tsukamoto, K. et. al.
  • Journal Title: Microb. Pathog. (in press)
  • Peer Reviewed
• [Journal Article] A nasal vaccine comprising B-subunit derivative of Shiga toxin 2 for cross-protection against Shiga toxin types 1 and 2 (2008)
  • Author(s): Tsuji, T. et. al.
  • Journal Title: Vaccine 26 Pages: 2092-2099
  • Peer Reviewed
• [Journal Article] Protective effect of botulinum C/D mosaic toxoid against avian botulism (2006)
  • Author(s): Takeda, M.
  • Journal Title: J.Vet.Med.Sci. 68巻・4号 Pages: 325-330
• [Presentation] ボツリヌスD型神経毒素とPhosphatidylethanolamineの相互作用の解析 (2009)
  • Author(s): 塚本健太郎
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 愛知県
  • Year and Date: 2009-03-14
• [Presentation] ボツリヌスD型神経毒素とPhosphatidylethanolamineの相互作用の解析 (2009)
  • Author(s): 塚本健太郎, 他
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-03-14
• [Presentation] ボツリヌス神経毒素受容体としての糖脂質およびリン脂質の役割 (2008)
  • Author(s): 塚本健太郎
  • Organizer: 第45回日本細菌学会中部支部総会
  • Place of Presentation: 石川県
  • Year and Date: 2008-10-18
• [Presentation] ボツリヌス神経毒素受容体としての糖脂質およびリン脂質の役割 (2008)
  • Author(s): 塚本健太郎, 他
  • Organizer: 第45回日本細菌学会中部支部総会
  • Place of Presentation: 金沢大学医学部記念館
  • Year and Date: 2008-10-18
• [Presentation] Surface plasmon resonance analysis of botulinum neurotoxin binding for lipid receptors (2008)
  • Author(s): 塚本健太郎
  • Organizer: TOXINS2008
  • Place of Presentation: イタリア
  • Year and Date: 2008-06-13
• [Presentation] Surface plasmon resonance analysis of botulinum neurotoxin binding for lipid recentors (2008)
  • Author(s): Tsukamoto, K., et.al.
  • Organizer: TOXINS 2008
  • Place of Presentation: Baveno, Italy
  • Year and Date: 2008-06-13
• [Presentation] 表面プラズモン共鳴を用いたボツリヌス神経毒素と受容体の結合解析 (2008)
  • Author(s): 塚本健太郎
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都府
  • Year and Date: 2008-03-24
• [Presentation] 表面プラズモン共鳴を用いたボツリヌス神経毒素と受容体の結合解析 (2008)
  • Author(s): 塚本健太郎
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-03-24
• [Presentation] ボツリヌスC, D型神経毒素と脂質分子の多様な結合性について (2007)
  • Author(s): 塚本健太郎
  • Organizer: 第54回毒素シンポジウム
  • Place of Presentation: 大阪府
  • Year and Date: 2007-09-06
• [Presentation] ボツリヌスC、D型神経毒素と脂質分子の多様な結合性について (2007)
  • Author(s): 塚本健太郎
  • Organizer: 第54回 毒素シンポジウム
  • Place of Presentation: 大阪府犬鳴グランドホテル紀泉閣
  • Year and Date: 2007-09-06
• [Presentation] Mutational analysis of botulinum neurotoxintype C and D for determination of receptor-binding site (2007)
  • Author(s): 塚本健太郎
  • Organizer: 第7回あわじしま感染症・免疫フォーラム
  • Year and Date: 2007-09-02
• [Presentation] Mutational analysis of botulinum neurotoxin type C and D for determination of receptor-binding site (2007)
  • Author(s): 塚本健太郎
  • Organizer: The 7th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 兵庫県淡路夢舞台国際会議場
  • Year and Date: 2007-09-02