| Project/Area Number |
18F18100
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Research Field |
Virology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
間 陽子 国立研究開発法人理化学研究所, 科技ハブ産連本部, 研究員 (50182994)
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| Co-Investigator(Kenkyū-buntansha) |
MAIREPATI PALATI 国立研究開発法人理化学研究所, 科技ハブ産連本部, 外国人特別研究員
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| Project Period (FY) |
2018-04-25 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2019: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2018: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | BLV / Sensitive PCR / NGS / DNA-Capture-Seq analysis / LM PCR Seq analysis / ヒト由来細胞株 / ヒト血液 / ヒト乳癌組織 / Bovine leukemia virus / human Blood / human cancer tissue / DNA extraction / PCR amplification / Next-generation sequencing / BLV-human hybrid genome / BLV integration site |
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| Outline of Annual Research Achievements |
We extracted DNA from 199 human cell lines and from 100 human blood and 27 breast cancer tissues sample. Samples were subjected to detect BLV presence in human by sensitive PCR. PCR result showed that all human cell lines were BLV negative. We observed BLV gene specific bands in 6 blood and 5 cancer tissue sample. To confirm the PCR result, we performed two different high throughput sequencing method, namely viral DNA-Capture-Seq and LM PCR library enhancement NGS. Both of the high throughput sequencing failed to detect any single read mapped to BLV sequencing. BLV detection results indicate that there is no evidence for BLV-specific sequences. The positive result in blood and cancer tissue samples might be caused by the cross contamination during the process of performing the experiment.
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| Research Progress Status |
令和元年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和元年度が最終年度であるため、記入しない。
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