Functional characterization of candidate genes for PUE and mining their allelic variants in rice
| Project/Area Number |
18F18703
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | Japan International Research Center for Agricultural Sciences |
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Principal Investigator |
Wissuwa Matthias 国立研究開発法人国際農林水産業研究センター, 生産環境・畜産領域, 主任研究員 (90442722)
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| Co-Investigator(Kenkyū-buntansha) |
ADEM GETNET 国立研究開発法人国際農林水産業研究センター, 生産環境・畜産領域, 外国人特別研究員
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| Project Period (FY) |
2018-07-25 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2020: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2019: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2018: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | PUE / P-efficient / P-inefficient / rice genotypes / P flux / RNA sequencing / transcriptome analysis / high biomass / metabolic forms of P / fractionation of P / expression / bioinformatics / QPCR / Gene 13 / Gene 14 / Gene 30 / PM-H+ATP / remobilization P |
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| Outline of Annual Research Achievements |
To breed rice plants for PUE (Phosphorus use efficiency), understanding the physiological, metabolic, molecular and genetic aspects of PUE is important. In our experiments, P-efficient and P-inefficient rice genotypes were used to understand the above-mentioned traits with different doses of P supply. P-efficient genotypes maintain low concentration of P and P content and high biomass that contributed to the efficiency of the rice plant in phosphorus utilization. In addition, the P-efficient rice genotypes showed P flux from old to young leaves. In another experiment, the P-efficient genotypes showed reallocation of P from shoot to root where sampling was done at six time intervals in approx. 2 days gap.
The fractionation of P into organic, nucleic acid, inorganic and metabolic components of P was studied in collaboration with Dr. Patrick Hayes (JSPS Fellow (P18711). Leaf cell-specific P-allocation was also another component looked at in this experiment. Similarly, transcriptome analysis using RNA sequencing was done for P-efficient and P-inefficient rice genotypes under different P supply. This experiment was done in collaboration with Dr. Asad Prodhan (JSPS Fellow (P19709)). These parts of the experiment will be reported by the above 2 JSPS fellows to avoid redundancy.
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| Research Progress Status |
令和2年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和2年度が最終年度であるため、記入しない。
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Report
(3 results)
Research Products
(1 results)
