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Functional characterization of candidate genes for PUE and mining their allelic variants in rice

Research Project

Project/Area Number 18F18703
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Plant nutrition/Soil science
Research InstitutionJapan International Research Center for Agricultural Sciences

Principal Investigator

Wissuwa Matthias  国立研究開発法人国際農林水産業研究センター, 生産環境・畜産領域, 主任研究員 (90442722)

Co-Investigator(Kenkyū-buntansha) ADEM GETNET  国立研究開発法人国際農林水産業研究センター, 生産環境・畜産領域, 外国人特別研究員
Project Period (FY) 2018-07-25 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2020: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2019: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2018: ¥800,000 (Direct Cost: ¥800,000)
KeywordsPUE / P-efficient / P-inefficient / rice genotypes / P flux / RNA sequencing / transcriptome analysis / high biomass / metabolic forms of P / fractionation of P / expression / bioinformatics / QPCR / Gene 13 / Gene 14 / Gene 30 / PM-H+ATP / remobilization P
Outline of Annual Research Achievements

To breed rice plants for PUE (Phosphorus use efficiency), understanding the physiological, metabolic, molecular and genetic aspects of PUE is important. In our experiments, P-efficient and P-inefficient rice genotypes were used to understand the above-mentioned traits with different doses of P supply. P-efficient genotypes maintain low concentration of P and P content and high biomass that contributed to the efficiency of the rice plant in phosphorus utilization. In addition, the P-efficient rice genotypes showed P flux from old to young leaves. In another experiment, the P-efficient genotypes showed reallocation of P from shoot to root where sampling was done at six time intervals in approx. 2 days gap.
The fractionation of P into organic, nucleic acid, inorganic and metabolic components of P was studied in collaboration with Dr. Patrick Hayes (JSPS Fellow (P18711). Leaf cell-specific P-allocation was also another component looked at in this experiment. Similarly, transcriptome analysis using RNA sequencing was done for P-efficient and P-inefficient rice genotypes under different P supply. This experiment was done in collaboration with Dr. Asad Prodhan (JSPS Fellow (P19709)). These parts of the experiment will be reported by the above 2 JSPS fellows to avoid redundancy.

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (1 results)

All 2020

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results)

  • [Journal Article] Genetic and Physiological Traits for Internal Phosphorus Utilization Efficiency in Rice Natural Genetic Variation2020

    • Author(s)
      Getnet Dino Adem, Yoshiaki Ueda, Patrick E Hayes, Matthias Wissuwa
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 11 Pages: e0241842-e0241842

    • DOI

      10.1371/journal.pone.0241842

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access

URL: 

Published: 2018-07-26   Modified: 2024-03-26  

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