| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2022: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2021: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Outline of Final Research Achievements |
In this project, we developed basic know-hows to perform genome editing using CRISPR/Cas9 in the limpet Nipponacmea fuscoviridis, and we also carried out experiments to unveil the genetic mechanisms underlying the shell spiral growth in molluscs. As a result, we successfully knocked out the lophotrochin gene which is known to be expressed in an early larval stage in the limpet. We also identified several shell matrix protein genes which are unevenly expressed between the right and left sides of the mantle, and could be related to the lateral asymmetry of the shell growth in the pond snail Lymnaea stagnalis. We further discovered that the signal transduction factor Wnt is responsible for the twisted growth of the shell during the spiral shell growth in L. stagnalis.
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