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Development of novel control technology for wastewater treatment process based on multi-omics data and machine learning

Research Project

Project/Area Number 18H01576
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 22060:Environmental systems for civil engineering-related
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Narihiro Takashi  国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (20421844)

Co-Investigator(Kenkyū-buntansha) 延 優  国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (40805644)
黒田 恭平  国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (50783213)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywords廃水処理 / 微生物 / 菌叢解析 / メタゲノム / 微生物群集構造 / 活性汚泥 / 微生物ゲノム / 機械学習 / 微生物代謝
Outline of Final Research Achievements

We aim to discover the control factors of wastewater treatment by employing microbial community structure, metagenome assembled genome, and operational data. The major outcomes are (1) The microbial community analysis in activated sludge processes during the operational period after an increase in the wastewater flow rate showed that increased flow rate has an impact on Nitrosomonas populations. (2) Ecogenomic analysis of lab-scale anaerobic reactor treating food-processing wastewater revealed the biodegradation flow of amino acids. (3) We constructed a lab-scale reactor to treat polyethylene terephthalate production wastewater and achieved high COD removal rate. In addition, we elucidated the mechanism of aromatic compounds degradation based on metagenomic analysis. Overall, functionally and phylogenetically diverse microorganisms unite to form a metabolic network to perform bioconversion of organic/inorganic compounds in wastewater sludge microbiota.

Academic Significance and Societal Importance of the Research Achievements

内閣府のバイオ戦略に謳われているバイオエコノミー社会の形成に向け、各種製造業における有機性廃水・廃棄物処理の効率化や高度化が求められている。その実現のために本研究では、廃水処理プロセスにおいて有機物分解や窒素除去といった生物反応の中核を担っているにもかかわらず、その代謝機能の全容が解明されていない微生物に着目し、都市下水や産業廃水を処理する好気性・嫌気性プロセスを対象として、微生物機能情報を菌叢、ゲノム、遺伝子発現といったマルチオミクスの観点から明らかにした。これらの成果から見出された微生物学的知見は、新しいリアクター設計や運転制御指針に向けた応用展開での活用が期待される。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (15 results)

All 2021 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results)

  • [Int'l Joint Research] 四川大学(中国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Ecogenomics Reveals Microbial Metabolic Networks in a Psychrophilic Methanogenic Bioreactor Treating Soy Sauce Production Wastewater2021

    • Author(s)
      Kuroda Kyohei、Narihiro Takashi、Nobu Masaru K.、Tobo Atsushi、Yamauchi Masahito、Yamada Masayoshi
    • Journal Title

      Microbes and Environments

      Volume: 36 Issue: 4 Pages: n/a

    • DOI

      10.1264/jsme2.ME21045

    • NAID

      130008094799

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Using DNA-based stable isotope probing to reveal novel propionate- and acetate-oxidizing bacteria in propionate-fed mesophilic anaerobic chemostats2019

    • Author(s)
      Wang Hui-Zhong、Lv Xiao-Meng、Yi Yue、Zheng Dan、Gou Min、Nie Yong、Hu Bing、Nobu Masaru K.、Narihiro Takashi、Tang Yue-Qin
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 17396-17396

    • DOI

      10.1038/s41598-019-53849-0

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of the Wastewater Flow Rate on Interactions between the Genus <i>Nitrosomonas</i> and Diverse Populations in an Activated Sludge Microbiome2019

    • Author(s)
      Narihiro Takashi、Nobu Masaru Konishi、Hori Tomoyuki、Aoyagi Tomo、Sato Yuya、Inaba Tomohiro、Aizawa Hidenobu、Tamaki Hideyuki、Habe Hiroshi
    • Journal Title

      Microbes and Environments

      Volume: 34 Issue: 1 Pages: 89-94

    • DOI

      10.1264/jsme2.ME18108

    • NAID

      130007627737

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] PET製造廃水処理UASB反応槽内保持汚泥に優占する門レベル未培養系統群の機能解明の試み2021

    • Author(s)
      前田 稜太, 野口 太郎, 成廣 隆, 黒田 恭平
    • Organizer
      第56回日本水環境学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 廃水処理の微生物生態から見えてくるもの2021

    • Author(s)
      成廣 隆
    • Organizer
      第34回日本微生物生態学会年会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Simplify and understand the wastewater treatment microbiome2021

    • Author(s)
      成廣 隆
    • Organizer
      The 9th Microbial Ecology & Water Engineering Specialist Conference, International Water Association (IWA)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高負荷PET製造廃水処理UASBリアクター内保持汚泥のマイクロバイオーム解析2021

    • Author(s)
      新島二葉, 吉田実桜, 山口 陽香, 蔵下はづき, 中原 望, NOBU MASARU KONISHI, 成廣 隆, 野口太郎, 山田真義, 山内正仁, 黒田 恭平
    • Organizer
      日本微生物生態学会第34 回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 汚泥マイクロバイオーム解析による廃水処理技術高度化への研究展開2019

    • Author(s)
      成廣隆
    • Organizer
      TIRIクロスミーティング2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] UASBリアクターによるPET製造複合模擬廃水の高負荷連続処理実験2019

    • Author(s)
      吉田実桜、山口陽香、蔵下はづき、黒田恭平、成廣 隆、NOBU MASARU KONISHI、山田真義
    • Organizer
      第54回日本水環境学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] プラスチック製造過程における複合廃水を対象としたUASB 反応槽による連続処理実験2019

    • Author(s)
      山口陽香,蔵下はづき,延優,成廣隆,山田真義,黒田恭平
    • Organizer
      水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 汚泥マイクロバイオーム解析による廃水処理技術高度化への研究展開2019

    • Author(s)
      成廣隆
    • Organizer
      産総研北海道センターワークショップ in 帯広
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Microbiome in Biological Wastewater Treatment Process.2019

    • Author(s)
      Takashi Narihiro
    • Organizer
      The 2nd TISTR and AIST Mini Joint Symposium, Thailand
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 廃水処理微生物の群集構造やメタゲノムデータに何を語らせよう?2018

    • Author(s)
      成廣隆,Masaru K. Nobu, 黒田恭平,山田真義
    • Organizer
      水環境学会シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 汚泥マイクロバイオームの世界を解き明かす2018

    • Author(s)
      成廣隆
    • Organizer
      BioJapan2018
    • Related Report
      2018 Annual Research Report
    • Invited

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Published: 2018-04-23   Modified: 2023-01-30  

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