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Innovation on in situ biogeochemical measurement with advanced flow-analysis and optical sensing technology

Research Project

Project/Area Number 18H01651
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 24020:Marine engineering-related
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

Fukuba Tatsuhiro  国立研究開発法人海洋研究開発機構, 研究プラットフォーム運用開発部門, 主任研究員 (80401272)

Co-Investigator(Kenkyū-buntansha) 岡村 慶  高知大学, 教育研究部総合科学系複合領域科学部門, 教授 (70324697)
下島 公紀  東京海洋大学, 学術研究院, 教授 (70371490)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Keywords海洋計測 / 現場計測 / 光センサ / 微弱光 / マイクロ流体デバイス / 環境計測 / ATP
Outline of Final Research Achievements

In this study, we aimed to realize ultra-compact in-situ measurement devices that can be used in the marine environment. In particular, we conducted research and development to realize flow-analyzers with state-of-the-art optical measurement technology. We adopted a micro PMT (photo multiplier tube) as a photodetector and the microfluidic device for ATP (adenosine triphosphate, proxy of microbial biomass) determination was miniaturized utilizing a 3D printer technology. Furthermore, we demonstrated that the micro PMT can be used underwater condition by applying oil-immersed configuration without a pressure-resistant housing. As a result of the above research and development, we achieved the ultra-miniaturization of the entire in situ ATP analyzer, and were able to demonstrate the possibility of simultaneous measurement of multiple environmental parameters by mounting it on a small desktop-size underwater robot together with various underwater sensors through the sea trial.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果を応用することで、海中で使用される分析装置の中でも、高感度な光計測を必要とする微量金属や生体関連成分の分析を目的とした装置の超小型化が可能になる。これにより、小型の海中プラットフォームへの分析装置の搭載が可能になるため、広大な海洋環境の網羅的な計測を目指す分散センシングなどに応用可能である。小型化された分析装置は、地球環境変動のより詳細な把握や新たな資源の探査および環境影響評価など、多様なミッションに適用可能であり、新たな海洋科学・海洋利用に資すると共に、人類社会の持続的な発展に貢献できる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (13 results)

All 2021 2020 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (11 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Lab-on-a-chip technology for in situ combined observations in oceanography2021

    • Author(s)
      Tatsuhiro Fukuba, Teruo Fujii
    • Journal Title

      Lab on a Chip

      Volume: 21 Issue: 1 Pages: 55-74

    • DOI

      10.1039/d0lc00871k

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] マイクロ流体技術による海洋化学・生化学計測(<特集>「地球環境の変化を知る―技術はどのように貢献するか―」)2018

    • Author(s)
      福場辰洋
    • Journal Title

      Journal of the Society of Mechanical Engineers

      Volume: 121 Issue: 1199 Pages: 28-31

    • DOI

      10.1299/jsmemag.121.1199_28

    • NAID

      130007584040

    • ISSN
      2424-2675
    • Year and Date
      2018-09-05
    • Related Report
      2018 Annual Research Report
  • [Presentation] 現場型ガンマ線センサによる外洋域での鉛直連続計測2021

    • Author(s)
      下島公紀
    • Organizer
      GEOTRACES-Japanシンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 紫外吸光光度法を用いた海水中硝酸 塩の現場分析法の検討2020

    • Author(s)
      宮本洋好, 岡村慶, 野口拓郎, 八田万有美
    • Organizer
      2020年度日本地球化学会第67回
    • Related Report
      2020 Annual Research Report
  • [Presentation] 紫外分光光度法を用いた海水中溶存 硝酸態窒素濃度の現場分析法開発2020

    • Author(s)
      宮本洋好, 岡村慶, 野口拓郎, 八田万有美
    • Organizer
      第80回日本分析化学会討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] オホーツク海南岸における長期連続観測へのpH/pCO2センサの適用2020

    • Author(s)
      下島公紀
    • Organizer
      日本海洋学会2020年度秋季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Development and improvement of miniaturized in situ bio/biochemical analysis systems towards multi-modal ocean sensing2019

    • Author(s)
      T. Fukuba, Y. Sano, H. Yamamoto, T. Miwa, and T. Fujii
    • Organizer
      Underwater Technology 2019 (UT'19)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 海洋現場におけるATP(アデノシン3リン酸)定量測定に向けた3Dプリンタ製マイクロ流体デバイスの 設計・評価2019

    • Author(s)
      佐野優貴, 三輪哲也, 福場辰洋
    • Organizer
      電気学会 マグネティックス・マイクロマシン・バイオマイクロシステム合同研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Application of 3D-Printed Microfluidic Device and Miniature Photodetection Technology Towards Photometry Based Biochemical Analysis in Deep Sea2019

    • Author(s)
      T. Fukuba, Y. Sano
    • Organizer
      The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sensor fusion to ocean observing platforms2019

    • Author(s)
      K Shitashima
    • Organizer
      OceanObs’19
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 小型分析装置による微生物バイオマスの現場定量と現場遺伝子解析への展開2019

    • Author(s)
      福場 辰洋, 佐野優貴, 藤井輝夫
    • Organizer
      ブルーアースサイエンス・テク2019
    • Related Report
      2018 Annual Research Report
  • [Presentation] 現場ATP定量用マイクロ流体デバイスの小型化 -試作と評価-2019

    • Author(s)
      佐野優貴, 福場 辰洋, 三輪哲也
    • Organizer
      ブルーアースサイエンス・テク2019
    • Related Report
      2018 Annual Research Report
  • [Presentation] 海中現場生物計測への挑戦と展開ーバイオマス計測から遺伝子分析へ2019

    • Author(s)
      福場 辰洋
    • Organizer
      ブルーアースサイエンス・テク2019
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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