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Studies on microbiologically influenced corrosion mechanisms by spatial - temporal imaging of an internal environment of biofilm

Research Project

Project/Area Number 18H01755
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 26050:Material processing and microstructure control-related
Research InstitutionOsaka City University

Principal Investigator

Kawakami Hiroshi  大阪市立大学, 大学院工学研究科, 准教授 (90305615)

Co-Investigator(Kenkyū-buntansha) 北村 昌也  大阪市立大学, 大学院工学研究科, 教授 (20244634)
中西 猛  大阪市立大学, 大学院工学研究科, 准教授 (20422074)
辻 幸一  大阪市立大学, 大学院工学研究科, 教授 (30241566)
有吉 欽吾  大阪市立大学, 大学院工学研究科, 准教授 (80381979)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2020: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2019: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Keywords微生物腐食 / メタ遺伝子解析 / 蛍光X線分析 / 電気化学分析 / バイオフィルム
Outline of Final Research Achievements

Volume of specific bacterial cells and extracellular polysaccharides in biofilm were quantified by using a fluorescent DNA probe and a confocal laser scanning microscope. Such microscopic technique was also useful to observe distribution of metal corrosive bacterial cells in the BF and local corrosion under the BF. It was also shown that pickling is effective as a countermeasure against microbial corrosion of stainless steel welds.
A cases study indicated that the Rhodocyclaceae sp. bacteria living in a BF increased the corrosion rate in a microaerobic environment. When a hypochlorous acid-based disinfectant was added to such a system, corrosion rate in a microaerophile environment significantly decreased, indicating that the hypochlorous acid-based disinfectant is effective as a countermeasure against microbially influenced corrosion.

Academic Significance and Societal Importance of the Research Achievements

バイオフィルム(BF)中の金属腐食性菌の定量化とその空間分布の可視化を可能にし,さらに可視化された金属腐食性菌と局部腐食の同時観察方法を確立した.この観察手法今後の微生物腐食の解析のみならず,ミクロな生態系としてのバイオフィルムの微生物学的研究にも応用できる.また,ステンレス鋼溶接部の微生物腐食と炭素鋼排水管の微生物腐食への対策を提案した.この成果は各種プラントやインフラ設備の安全使用に資する.

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (10 results)

All 2021 2020 2019 2018

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] 微生物による劣化ー繊維,金属,木材,コンクリート,紙などー1 微生物による材料の劣化ー概要ー2021

    • Author(s)
      川上洋司
    • Journal Title

      日本防菌坊黴学会誌

      Volume: 49 Pages: 137-148

    • Related Report
      2020 Annual Research Report
  • [Journal Article] 溶接部の腐食トラブル事例と要因解析・対策ー溶接冶金委員会ー微生物腐食事例とその対策2020

    • Author(s)
      川上洋司
    • Journal Title

      溶接学会誌

      Volume: 89 Pages: 69-70

    • Related Report
      2020 Annual Research Report
  • [Journal Article] 鉄鋼材料の微生物腐食挙動の 共焦点微小部蛍光X 線分析法による観察2019

    • Author(s)
      陳自義,細見凌平,川上洋司,辻幸一
    • Journal Title

      X線分析の進歩

      Volume: 50 Pages: 169-175

    • NAID

      40021864047

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 微生物腐食概論2018

    • Author(s)
      川上洋司
    • Journal Title

      環境管理技術

      Volume: 36 Pages: 199-210

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Effect of Pickling and Mechanical Grinding on Initiation of Biofilm and Microbially Influenced Corrosion of Stainless Steel Weldments2019

    • Author(s)
      H. Kawakami, T. Wada, T. Nakanishi, K. Ariyoshi, K. Tsuji, M. Kitamura, and Y. Kikuchi
    • Organizer
      NACE East Asia & Pacific Area Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ステンレス鋼溶接部でのバイオフィルム発生と微生物腐食2019

    • Author(s)
      川上洋司,西岡祐貴,和田卓大,菊地靖志
    • Organizer
      第66回材料と環境討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 酸洗や機械研磨がステンレス鋼溶接部でのバイオフィルム発生挙動と微生物腐食発生に及ぼす影響2019

    • Author(s)
      川上洋司,和田卓大,菊地靖志
    • Organizer
      材料と環境2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] ステンレス鋼溶接部の微生物腐食2019

    • Author(s)
      川上洋司,菊地靖志
    • Organizer
      溶接学会2019年度秋季全国大会 フォーラム”溶接部の腐食”
    • Related Report
      2019 Annual Research Report
  • [Presentation] 微生物腐食概論2018

    • Author(s)
      川上洋司
    • Organizer
      日本材料学会腐食防食部門委員会第321例会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 多種類の菌で形成されたバイオフィルムの付着量とステンレス鋼溶接部の腐食の発生との関係2018

    • Author(s)
      和田卓大,川上洋司
    • Organizer
      日本鉄鋼協会第176回秋季講演大会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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