Development of a platform technology for intracellular drug discovery
| Project/Area Number |
18H01792
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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| Research Institution | National Institutes of Biomedical Innovation, Health and Nutrition (2019-2020)
The University of Tokyo (2018) |
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Principal Investigator |
KAWAHARA Masahiro 国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 ワクチン・アジュバント研究センター, プロジェクトリーダー (50345097)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | 蛋白質 / 相互作用 / スクリーニング / シグナル伝達 / 動物細胞 / タンパク質 |
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| Outline of Final Research Achievements |
In this study, we have developed a new intracellular protein-protein interaction detection technology that detects cell proliferation associated with intracellular protein-protein interactions using a receptor or a signaling molecule downstream of receptors. In this method, the detection sensitivity of protein-protein interactions could be adjusted by adding a small molecule. Using this method, peptide-protein domain interactions could be detected generally, and high-affinity intracellular antibodies could be screened from a library.
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| Academic Significance and Societal Importance of the Research Achievements |
ガンや感染症などの疾患は現代社会における脅威であるが、種々の疾患に対する効果的な治療薬は未だ確立されていないのが現状である。一方で、生体内のプロセスはほぼ全てがタンパク質間相互作用に支配されており、タンパク質間相互作用を標的とした創薬は無限の可能性がある。本研究では、種々の疾患の原因となるタンパク質間相互作用を標的として、生きた細胞内での治療薬のスクリーニング技術を開発したことから、学術的・社会的意義は高い。
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Chimeric G-CSF receptor-mediated STAT3 activation contributes to efficient induction of cardiomyocytes from mouse induced pluripotent stem cells.2019
Author(s)
Tsukamoto T, Sogo T, Ueyama T, Nakao S, Harada Y, Ihara D, Akagi Y, Kida YS, Hasegawa K, Nagamune T, Kawahara M, Kawamura T.
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Journal Title
Biotechnol J
Volume: -
Issue: 2
Pages: 1900052-1900052
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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