Microvessel system for the analysis of stem cell maintenance in tissue
Project/Area Number |
18H01793
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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Research Institution | The University of Tokyo |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
川辺 淳一 旭川医科大学, 医学部, 教授 (10400087)
長谷川 洋介 東京大学, 生産技術研究所, 准教授 (30396783)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
|
Keywords | 血管新生 / ペリサイト / 流体シミュレーション / 三次元モデル / マイクロデバイス / 組織幹細胞 / 血管再生 / 3次元モデル / 毛細血管 / in vitroモデル / 周皮細胞 |
Outline of Final Research Achievements |
The purpose of this study is "construction of a microvascular system for elucidating the tissue stem cell maintenance mechanism". With the increase in lifestyle-related diseases such as hypertension, diabetes, and hyperlipidemia, it is predicted that the frequency of arteriosclerosis obliterans and critical limb ischemia will increase in addition to myocardial infarction and stroke. For these reasons, there are high expectations for angiogenic therapy. In this study, we focused on pluripotent capillary stem cells (CapSC) and changed various physicochemical factors in an in vitro three-dimensional microvascular model. We constructed a bioprocess evaluation system at each stage regarding the role of CapSC in angiogenesis (promotion of angiogenesis or stabilization of blood vessels). The results are expected to elucidate the mechanism of tissue stem cell maintenance and to be applied to efficient angiogenic therapy by cell transplantation.
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Academic Significance and Societal Importance of the Research Achievements |
In vitro三次元微小血管デバイスを用いることで、生体内ではブラックボックスであった各細胞、外的環境との相互作用を見える化することができる。ペリサイトとCapSCなど、細胞の種類を変えたときの血管新生過程を確認できるため、構成論的なアプローチで生体の現象を模擬できる手法は極めて斬新であり、適切かつ効率的な血管新生療法の提案に貢献できる。
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Report
(4 results)
Research Products
(70 results)
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[Journal Article] Multiscale parareal algorithm for long-time mesoscopic simulations of microvascular blood flow in Zebrafish2021
Author(s)
Blumers, A., Yin, M., Nakajima, H., Hasegawa, Y., Li, Z., Karniadakis, G.E.
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Journal Title
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Numerical evaluation reveals the effect of branching morphology on vessel transport properties during angiogenesis2021
Author(s)
Mirzapour-shafiyi, F., Kametani, Y., Hikita, T., Hasegawa, Y., Nakayama, M.,
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Journal Title
Plos Computational Biology
Volume: -
Pages: 1-16
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] TGF-β and TNF-α cooperatively induce mesenchymal transition of lymphatic endothelial cells via activation of Activin signals2020
Author(s)
Yoshimatsu Y, Kimuro S, Pauty J, Takagaki K, Nomiyama S, Inagawa A, Maeda K, Podyma-InoueKA, Kajiya K, Matsunaga Y, Watabe T
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Journal Title
PLOS ONE
Volume: 15
Issue: 5
Pages: e0232356-e0232356
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Transcriptomic Analysis of Breast Cancer Stem Cells and Development of a pALDH1A1:mNeptune Reporter System for Live Tracking2019
Author(s)
N. Bidan, J. Bailleul-Dubois, J. Duval, M. Winter, M. Denoulet, K. Hannebicque, IY El-Sayed, C. Ginestier, V. Forissier, Y.T. Matsunaga, S. Meignan, F. Anquez, S. Julien, A. Bonnefond, M. Derhourhi, X.L., Bourhis, C. Lagadec
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Journal Title
Proteomics
Volume: 19
Issue: 21-22
Pages: 21-22
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] EphA7陽性周細胞は、筋ジストロフィーモデルマウスの病態を改善する.2020
Author(s)
鹿野耕平, 堀内至, 吉田有里, 早坂太希, 鹿原真樹, 富田唯, 竜川貴光, 松尾梨沙, 安田哲, 澤田潤, 中川直樹, 竹原有史, 長谷部直幸, 川辺淳一
Organizer
第19回日本再生医療学会総会
Related Report
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[Presentation] Transplantation of capillary stem cells improves skeletal muscle regeneration in muscular dystrophy.2020
Author(s)
Kohei Kano, Kiwamu Horiuchi, Yuri Yoshida, Taiki Hayasaka, Maki Kabara, Yui Tomita, Takamitsu Tatsukawa, Risa Matsuo, Satoshi Yasuda, Jun Sawada, Naoki Nakagawa, Naofumi Takehara, Naoyuki Hasebe, and Jun-ichi Kawabe
Organizer
第61回日本神経学会学術大会
Related Report
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