Development of sophisticated biosynthetic process for magnetic nanoparticles by using intracellular compartment

• Project/Area Number: 18H01794
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 27040:Biofunction and bioprocess engineering-related
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Arakaki Atsushi 東京農工大学, 工学(系)研究科(研究院), 准教授 (10367154)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: 磁性ナノ粒子 / コンパートメント / バイオプロセス / 遺伝子組換え / 細菌 / バイオミネラリゼーション / 遺伝子発現制御 / 生体機能利用 / ナノ材料合成 / 反応プロセス制御

Outline of Final Research Achievements

In this study, the regulation of manufacturing process for magnetic nanoparticles was investigated by using compartment in genetically modified magnetotactic bacteria. The method for introduction of large DNA fragment into the genome of magnetotactic bacteria was established. Control of shape, size and number of magnetic particles were shown by designing introduced gene sets or regulating gene expressions. Moreover, composition of magnetic particles was modified by the expression of metal ion transporter gene in the cell.

Academic Significance and Societal Importance of the Research Achievements

本研究では、磁性細菌の遺伝子を設計することで、磁気微粒子の形態・サイズ・組成と磁気特性の制御が可能であることを示した。遺伝子やゲノムに基づいてバイオプロセスを設計し、無機結晶合成を制御した世界初の例である。今後、酸化鉄磁気微粒子のみならず多様な無機結晶のバイオ合成法の開発に繋がる可能性があり、学術的・社会的に意義のある成果である。

Research Products

• [Journal Article] Restoration and Modification of Magnetosome Biosynthesis by Internal Gene Acquisition in a Magnetotactic Bacterium (2020)
  • Author(s): Atsushi Arakaki, Mayu Goto, Mina Maruyama, Takuto Yoda, Masayoshi Tanaka, Ayana Yamagishi, Yasuo Yoshikuni, Tadashi Matsunaga
  • Journal Title: Biotechnology Journal Volume: 15 Issue: 12 Pages: 2000278-2000278
  • DOI: 10.1002/biot.202000278
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Radular Stylus of Cryptochiton Stelleri: A Multifunctional Lightweight and Flexible Fiber-Reinforced Composite (2020)
  • Author(s): Anna Pohl, Steven A. Herrera, David Restrepo, Ryo Negishi, Jae-Young Jung, Chris Salinas, Richard Wuhrer, Tomoko Yoshino, Joanna McKittrick, Atsushi Arakaki, Michiko Nemoto, Pablo Zavattieri, David Kisailus
  • Journal Title: J Mech Behav Biomed Mater Volume: 111 Pages: 103991-103991
  • DOI: 10.1016/j.jmbbm.2020.103991
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Crystallization by particle attachment is a colloidal assembly process (2020)
  • Author(s): Mirabello, G, Ianiro, A, Bomans, PHH, Yoda, T, Arakaki, A, Friedrich, H,de With, G, Sommerdijk, NAJM
  • Journal Title: NATURE MATERIALS Volume: 19 Issue: 4 Pages: 391-396
  • DOI: 10.1038/s41563-019-0511-4
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Enhanced Tubulation of Liposome Containing Cardiolipin by MamY Protein from Magnetotactic Bacteria (2018)
  • Author(s): Masayoshi Tanaka, Thanawat Suwatthanarak, Atsushi Arakaki, Benjamin R. G. Johnson, Stephen D. Evans, Mina Okochi, Sarah S. Staniland, Tadashi Matsunaga
  • Journal Title: Biotechnology Journal Volume: 13 Issue: 12
  • DOI: 10.1002/biot.201800087
  • Peer Reviewed / Int'l Joint Research
• [Presentation] キチン積層構造形成機構の解明に向けた甲虫上翅のプロテオーム解析 (2020)
  • Author(s): 村田智志
  • Organizer: 第10回CSJ化学フェスタ2020
• [Presentation] 結晶形態制御タンパク質Mms6の金属酸化物ナノ粒子への吸着比較 (2020)
  • Author(s): 新井康祐
  • Organizer: 第10回CSJ化学フェスタ2020
• [Presentation] Regulation of Magnetite Biomineralization in Bacteria by Gene Rearrangement (2019)
  • Author(s): Atsushi Arakaki, Mayu Goto, Mina Maruyama, Takuto Yoda, Tadashi Matsunaga
  • Organizer: International Marine Biotechnology Conference 2019
  • Int'l Joint Research
• [Presentation] Comparative Adsorption Study of MMS Proteins from Magnetotactic Bacteria to Metal Oxide Nanoparticles (2019)
  • Author(s): Kosuke Arai, Satoshi Murata, Tadashi Matsunaga, Atsushi Arakaki
  • Organizer: International Marine Biotechnology Conference 2019
  • Int'l Joint Research
• [Presentation] 磁性細菌における磁気微粒子の合成機構とバイオ計測への応用 (2019)
  • Author(s): 新垣篤史
  • Organizer: 日本磁気科学会 2019年有機・バイオ分科会研究会
  • Invited
• [Presentation] 磁性細菌のマグネトソーム遺伝子領域の改変による磁気微粒子合成能の制御 (2019)
  • Author(s): 新垣篤史、丸山実菜、依田卓東、松永是
  • Organizer: 第71回日本生物工学会大会
• [Presentation] 遺伝子組換えによる磁性細菌の磁気微粒子合成能の改変 (2019)
  • Author(s): 新垣篤史、丸山実菜、依田卓東、松永是
  • Organizer: 第14回バイオミネラリゼーションワークショップ
  • Invited
• [Presentation] Reconstruction of magnetosome formation ability in non-magnetic mutant of Magnetospirillum magneticum AMB-1 (2018)
  • Author(s): Atsushi Arakaki, Tadashi Matsunaga
  • Organizer: The 6th International Meeting on Magnetotactic Bacteria
  • Int'l Joint Research / Invited
• [Presentation] Iron uptake and magnetosome formation abilities in MAI deletion mutant and genetically modified strain carrying multiple sets of MAI genes (2018)
  • Author(s): Takuto Yoda, Masayoshi Tanaka, Tadashi Matsunaga, Atsushi Arakaki
  • Organizer: The 6th International Meeting on Magnetotactic Bacteria
  • Int'l Joint Research
• [Presentation] Adsorption Study of Mms6 and Mms7 to Iron Oxide Magnetic Nanoparticles (2018)
  • Author(s): Kosuke Arai, Wataru Yoshimura, Satoshi Murata, Tadashi Matsunaga, Atsushi Arakaki
  • Organizer: The 6th International Meeting on Magnetotactic Bacteria
  • Int'l Joint Research
• [Presentation] Gene-based design and synthesis of biological magnetic nanoparticles (2018)
  • Author(s): Atsushi Arakaki
  • Organizer: Materials Science and Engineering congress
  • Int'l Joint Research / Invited
• [Presentation] 弾丸状磁気微粒子合成機構の解析に向けた長鎖DNAの合成 (2018)
  • Author(s): 篠原奈々美、松永是、新垣篤史
  • Organizer: 第8回CSJ化学フェスタ2018
• [Book] Chapter 2: Molecular mechanism of magnetic crystal formation in magnetotactic bacteria: In Biological magnetic materials and applications (2018)
  • Author(s): Atsushi Arakaki, Masayoshi Tanaka, Tadashi Matsunaga
  • Total Pages: 29
  • Publisher: Springer
  • ISBN: 9789811080685
• [Remarks] 生命分子工学・海洋生命工学研究室
  • URL: http://web.tuat.ac.jp/~biomol/index.html
• [Remarks] 東京農工大学 生命分子工学・海洋生物工学研究室
  • URL: http://web.tuat.ac.jp/~biomol/index.html