| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2021: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
In this study, we aimed to construct mosaic-like skeletal muscle tissue in vitro by taking advantage of the biological characteristic that the firing frequency of motor neurons connecting slow- and fast-twitch muscles is different, and developed the necessary technologies. First, we developed a two-dimensional co-cultured microdevice. Second, we developed a three-dimensional co-culture microdevice. Third, we found that the frequency of junction formation with co-cultured motor neurons can be improved by culturing skeletal muscle cells in an oriented manner. We plan to utilize these findings to further advance the construction of mosaic-like muscle tissue.
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