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Fabrication of 3D tissues including vascular-like network using bioinks gellable and removable through enzymatic reactions

Research Project

Project/Area Number 18H01797
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 27040:Biofunction and bioprocess engineering-related
Research InstitutionOsaka University

Principal Investigator

SAKAI SHINJI  大阪大学, 基礎工学研究科, 教授 (20359938)

Co-Investigator(Kenkyū-buntansha) 中畑 雅樹  大阪大学, 基礎工学研究科, 助教 (40755641)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Keywords再生医療 / 組織工学 / バイオプリンティング / 3Dプリンティング / 生物化学工学
Outline of Final Research Achievements

In this study, we attempted to develop methods for constructing functional 3D tissues by 3D bioprinting using a series of inks that is gellable and removable by enzymatic reactions. In order to achieve this, we developed a new 3D bioprinting method and showed that the inks can be used to fabricate structures without impairing the differentiation ability of human adipose-derived stem cells using visible light-responsive photocatalysts as well as enzymes. Furthermore, by applying the ink to an inkjet 3D printer, it was shown that microsphere-shaped and microfiber-shaped tissues can be fabricated. These results are expected to lead to the construction of tissue bodies containing vascular networks.

Academic Significance and Societal Importance of the Research Achievements

3Dプリンティングは、あたらしい造形技術として様々な分野で今後の活用が期待されている。再生医療分野においても、細胞を含むインクを使って構造物の造形を行う3Dプリンティングには大きな期待が寄せられている。本研究成果は、機能的な組織体を3Dプリンティングによって作製するにあたって不可欠な要素技術を開発したというものであり、今後、生体外で作製した組織体を用いる再生医療分野の発展に寄与すると期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (44 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (12 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Open Access: 7 results) Presentation (27 results) (of which Int'l Joint Research: 11 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Int'l Joint Research] University of Clermont Auvergne/University of Picardie Jules Verne(フランス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Clermont Auvergne/University of Picardie Jules Verne(フランス)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Influence of Hydrogen Peroxide-Mediated Cross-Linking and Degradation on Cell-Adhesive Gelatin Hydrogels2021

    • Author(s)
      Mubarok Wildan、Qu Yanfei、Sakai Shinji
    • Journal Title

      ACS Applied Bio Materials

      Volume: 1 Issue: 5 Pages: 0-0

    • DOI

      10.1021/acsabm.0c01675

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] settings Open AccessArticle Visible Light-Curable Chitosan Ink for Extrusion-Based and Vat Polymerization-Based 3D Bioprintings2021

    • Author(s)
      Mitsuyuki Hidaka、Masaru Kojima、Masaki Nakahata、Shinji Sakai
    • Journal Title

      Polymers

      Volume: 13 Pages: 1382-1382

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] バイオ3D プリンティング技術の現状と展望(特集:Additive Manufacturing技術の現在II)2020

    • Author(s)
      小嶋勝, 境慎司
    • Journal Title

      化学工学

      Volume: 84 Pages: 228-231

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Silk fibroin nanofibers: a promising ink additive for extrusion three-dimensional bioprinting2020

    • Author(s)
      Sakai S.、Yoshii A.、Sakurai S.、Horii K.、Nagasuna O.
    • Journal Title

      Materials Today Bio

      Volume: 8 Pages: 1-7

    • DOI

      10.1016/j.mtbio.2020.100078

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications2020

    • Author(s)
      Furuno Kotoko、Wang Jing、Suzuki Keiichiro、Nakahata Masaki、Sakai Shinji
    • Journal Title

      ACS Omega

      Volume: 5 Issue: 33 Pages: 21254-21259

    • DOI

      10.1021/acsomega.0c03164

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation2020

    • Author(s)
      Gantumur Enkhtuul、Nakahata Masaki、Kojima Masaru、Sakai Shinji
    • Journal Title

      International Journal of Bioprinting

      Volume: 6 Issue: 1 Pages: 72-81

    • DOI

      10.18063/ijb.v6i1.250

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors2019

    • Author(s)
      Sakai Shinji、Ohi Hiromi、Taya Masahito
    • Journal Title

      Biomolecules

      Volume: 9 Issue: 8 Pages: 342-342

    • DOI

      10.3390/biom9080342

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inkjet micropatterning through horseradish peroxidase-mediated hydrogelation for controlled cell immobilization and microtissue fabrication2019

    • Author(s)
      Gantumur, Enkhtuul, Kimura, Miyu, Taya, Masahito, Horie, Masanobu, Nakamura, Makoto, Sakai, Shinji
    • Journal Title

      Biofacrication

      Volume: 12 Issue: 1 Pages: 011001-011001

    • DOI

      10.1088/1758-5090/ab3b3c

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Use of Anionic Polysaccharides in the Development of 3D Bioprinting Technology2019

    • Author(s)
      Tai Chia、Bouissil Soukaina、Gantumur Enkhtuul、Carranza Mary Stephanie、Yoshii Ayano、Sakai Shinji、Pierre Guillaume、Michaud Philippe、Delattre C?dric
    • Journal Title

      Applied Sciences

      Volume: 9 Issue: 13 Pages: 2596-2596

    • DOI

      10.3390/app9132596

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Characterization of encapsulated cells within hyaluronic acid and alginate microcapsules produced via horseradish peroxidase-catalyzed crosslinking2019

    • Author(s)
      Khanmohammadi Mehdi、Sakai Shinji、Taya Masahito
    • Journal Title

      Journal of Biomaterials Science, Polymer Edition

      Volume: 30 Issue: 4 Pages: 295-307

    • DOI

      10.1080/09205063.2018.1562637

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Horseradish peroxidase-catalyzed hydrogelation consuming enzyme-produced hydrogen peroxide in the presence of reducing sugars2019

    • Author(s)
      Gantumur Enkhtuul、Sakai Shinji、Nakahata Masaki、Taya Masahito
    • Journal Title

      Soft Matter

      Volume: 15 Issue: 10 Pages: 2163-2169

    • DOI

      10.1039/c8sm01839a

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Peroxidase-catalyzed microextrusion bioprinting of cell-laden hydrogel constructs in vaporized ppm-level hydrogen peroxide2018

    • Author(s)
      Sakai Shinji、Mochizuki Kei、Qu Yanfei、Mail Matthew、Nakahata Masaki、Taya Masahito
    • Journal Title

      Biofabrication

      Volume: 10 Issue: 4 Pages: 045007-045007

    • DOI

      10.1088/1758-5090/aadc9e

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] 可視光照射により過酸化水素を生成させる光触媒を用いたヒドロゲルの作製2021

    • Author(s)
      北谷 優樹・ 白石 康浩・ 中畑 雅樹・ 小嶋 勝・ 平井 隆之・ 境 慎司
    • Organizer
      化学工学会第23回学生発表会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 低粘度シルクフィブロインインクの3Dバイオプリンティング2021

    • Author(s)
      境 慎司・ 森田 崇裕・ 中畑 雅樹・ 小嶋 勝
    • Organizer
      化学工学会第86年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Influence of hydrogen peroxidase-mediated cross-linking and degradation on cell-adhesive gelatin hydrogels2021

    • Author(s)
      Mubarok Wildan・ Qu Yanfei・ Nakahata Masaki・ Kojima Masaru・ Sakai Shinji
    • Organizer
      化学工学会第86年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 高精度なバイオインク切り替えのための単一ノズルの流路設計と評価2021

    • Author(s)
      日高 光将・ 山本 誠士郎・ 小嶋 勝・ 中畑 雅樹・ 岡野 泰則・ 境 慎司
    • Organizer
      化学工学会第86年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 多糖類のフェノール誘導体を基盤とした組織構築を目指したバイオプリンティング技術の開発2020

    • Author(s)
      日高光将、小嶋勝、中畑雅樹、境慎司
    • Organizer
      ロボティクス・メカトロニクス 講演会 2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] コリンオキシダーゼとペルオキシダーゼを利用した3Dバイオプリンティングの最適化2020

    • Author(s)
      原田涼平, 中畑雅樹, 小嶋勝, 境慎司
    • Organizer
      化学工学会 広島大会2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] ゼラチンスラリーを用いたシルクフィブロイン構造体の3Dバイオプリンティング2020

    • Author(s)
      森田 崇裕、吉井 彩乃、中畑 雅樹、小嶋 勝、境 慎司
    • Organizer
      化学工学会 広島大会2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] シルクフィブロインインクを用いた3Dバイオプリンティング技術の開発2020

    • Author(s)
      森田崇裕、吉井彩乃、境慎司
    • Organizer
      化学工学会 第85年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] キトサン&キトサンナノファイバー含有インクを用いた創傷被覆材の3Dプリンティング2020

    • Author(s)
      山本翔太・境慎司
    • Organizer
      化学工学会 第85年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シルクフィブロインナノファイバー含有インクを用いた3Dバイオプリンティング2020

    • Author(s)
      吉井彩乃, 永砂修, 櫻井俊輔, 境慎司
    • Organizer
      化学工学会 第85年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 2つの酵素反応を組み合わせた3Dバイオプリンティング技術の開発2020

    • Author(s)
      原田 涼平、山本翔太、Delattre Cedric、境 慎司
    • Organizer
      化学工学会 第85年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 様々なゲル基板上における細胞遊走試験のためのinkjet式バイオプリンティングによる細胞パターニング2020

    • Author(s)
      木村美夢、 Enkhtuul Gantumur、Mubarok Wildan、境 慎司
    • Organizer
      化学工学会 第85年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Inkjet plus enzyme-mediated bioprinting for controlled cell immobilization on substrates2019

    • Author(s)
      Miyu Kimura, Enkhtuul Gantumur, Shinji Sakai, Masahito Taya
    • Organizer
      The 14th Asian Congress on Biotechnology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of Nanofiber Non-Woven Fabric Applicable for Genome Editing in Vivo2019

    • Author(s)
      Kotoko Furuno, Shinji Sakai, Keiichiro Suzuki, Masahito Taya
    • Organizer
      The 14th Asian Congress on Biotechnology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Horseradish Peroxidase-Catalyzed Hydrogelation Consuming Enzyme-Produced Hydrogen Peroxide in the Presence of Reducing Sugars2019

    • Author(s)
      Enkhtuul Gantumur, Shinji Sakai, Masahito Taya
    • Organizer
      The 14th Asian Congress on Biotechnology
    • Related Report
      2019 Annual Research Report
  • [Presentation] Development of Bioinks Containing Nanofibrillated Chitosan for Extrusion 3D Bioprinting2019

    • Author(s)
      Shinji Sakai, Koji Hattori, Shota Yamamoto, Masahito Taya
    • Organizer
      The 14th Asian Congress on Biotechnology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Preparation of alginate and chitosan water-insoluble nanofibers through electrospinning and horseradish peroxidase-mediated crosslinking2019

    • Author(s)
      Jing Wang, Masahito Taya, Shinji Sakai
    • Organizer
      Asian Pacific Confederation of Chemical Engineering 2019 (APCChE 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication of disk and filament tissues through inkjet printing and enzymatic reaction2019

    • Author(s)
      Enkhtuul Gantumur, Miyu Kimura, Masahito Taya, Shinji Sakai
    • Organizer
      Asian Pacific Confederation of Chemical Engineering 2019 (APCChE 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of bioinks containing nanofibrillated chitosan for extrusion 3D bioprinting2019

    • Author(s)
      Shinji Sakai, Koji Hattori, Shota Yamamoto, Masahito Taya
    • Organizer
      Asian Pacific Confederation of Chemical Engineering 2019 (APCChE 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 創傷被覆材への応用を目指したキトサン + キトサンナノファイバーインクを用いた3Dプリンティング2019

    • Author(s)
      山本翔太 境慎司
    • Organizer
      日本バイオマテリアル学会関西ブロック 第14回若手研究発表会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ポリシクロデキストリンを含有する擬塑性バイオインクの開発2019

    • Author(s)
      山本翔太、中畑雅樹、境慎司
    • Organizer
      第41回日本バイオマテリアル学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シルクフィブロインナノファイバーを主要な成分とする三次元組織構築のためのバイオインクの開発2019

    • Author(s)
      吉井彩乃, 永砂修, 櫻井俊輔, 境慎司
    • Organizer
      第41回日本バイオマテリアル学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Horseradish Peroxidase-mediated Bioprinting in Air Containing Hydrogen Peroxide2019

    • Author(s)
      Shinji Sakai, Shota Yamamoto, Ayano Yoshii
    • Organizer
      The 16th Pacific Polymer Conference (PPC16)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 3D Printing of Cell-laden Hydrogels Through Visible Light-initiated Crosslinking2019

    • Author(s)
      Hidaka Mitsuyuki,Kojima Masaru, Sakai Shinji
    • Organizer
      The 16th Pacific Polymer Conference (PPC16)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Micro-extrusion bioprinting mediated by horseradish peroxidase2018

    • Author(s)
      Shinji Sakai, Kei Mochizuki, Masahito Taya
    • Organizer
      CHISA2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multi-ink inkjet bioprinting for cell- patterning through enzymatic cross-linking2018

    • Author(s)
      Gantumur Enkhtuul, Miyu Kimura, Shinji Sakai, Masahito Taya, Masato Nakamura
    • Organizer
      Biofabrication 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Horseradish peroxidase-mediated Micro-extrusion Bioprinting in Air Containing Vaporized Hydrogen Peroxide2018

    • Author(s)
      Shinji Sakai, Kei Mochizuki, Masahito Taya
    • Organizer
      Biofabrication 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Book] バイオ3Dプリント関連技術の開発と応用2019

    • Author(s)
      境慎司
    • Total Pages
      269
    • Publisher
      シーエムシー出版
    • ISBN
      9784781314327
    • Related Report
      2019 Annual Research Report
  • [Remarks] 再生医療への貢献 3Dバイオプリンティングに関する技術の開発

    • URL

      http://www.cheng.es.osaka-u.ac.jp/sakailabo/research_.html

    • Related Report
      2020 Annual Research Report
  • [Patent(Industrial Property Rights)] Ink for 3D printing system2019

    • Inventor(s)
      境慎司、吉井彩乃、永砂修、堀井和輝
    • Industrial Property Rights Holder
      境慎司、吉井彩乃、永砂修、堀井和輝
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
    • Overseas

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Published: 2018-04-23   Modified: 2022-01-27  

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