| Budget Amount *help |
¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
To exploit the utility of centrifugation-controlled thermal convection in biosensing applications, we aimed to construct a microfluidic device which has integrated reaction chambers. The device structure, integrated microchannel, and heater shape were designed based on the analyzed simulation result of heat transfer and fluid behavior in the model microenvironment. The prototype of the integrated microchannel chip and the rotating heater stage were fabricated and on-chip PCR was performed. With the developed system, rapid detection of multiple drug resistance genes was successfully demonstrated. In addition, the on-chip centrifugal thermal convection system was also applicable to accelerate antigen-antibody reaction (ELISA). With ease of operation and versatility for a range of targets, it is expected that this technology can be applied in a number of bioassays and enhance their value.
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