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The biocatalytic CO2-fixation catalyzed by (de)carboxylases and application to the production of various aromatic carboxylic acids

Research Project

Project/Area Number 18H01802
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 27040:Biofunction and bioprocess engineering-related
Research InstitutionWaseda University

Principal Investigator

Kino Kuniki  早稲田大学, 理工学術院, 教授 (60318764)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Keywords可逆的脱炭酸酵素 / 炭酸固定酵素 / 芳香族カルボン酸 / 芳香族ジカルボン酸 / フランジカルボン酸 / 芳香族モノカルボン酸 / 4-ヒドロキシイソフタル酸 / プロトカテク酸 / 4-ヒドロキシ安息香酸 / 炭酸固定 / カルボキシ基導入酵素 / ヒドロキシ基導入酵素
Outline of Final Research Achievements

With the aim of developing bioprocesses that will contribute to the realization of a decarbonized society, we have developed methods for the production of functionalized chemical products by introducing functional groups such as carboxy groups into aromatic compounds using non-oxidative reversible decarboxylases. In particular, we have promoted the search for a variety of carbonic anhydrase enzymes with different substrate specificities and expanded the range of compounds that can be synthesised, including aromatic carboxylic acids and aromatic dicarboxylic acids. We have also succeeded in developing a process for the synthesis of 2,5-furandicarboxylic acid, a raw material for poly(ethylene furanoate), which is attracting attention as a new bio-resin, from furfural.

Academic Significance and Societal Importance of the Research Achievements

我々の生活を豊かにしている多様な機能性化成品の多くは、依然として化石資源を原料とする従来型の化学工業プロセスに依存しており、二酸化炭素の純増を招く本プロセスは低炭素社会への流れに逆行している。本研究成果である微生物由来可逆的脱炭酸酵素の探索と各種芳香族カルボン酸合成法の開発は、二酸化炭素を芳香族化合物に固定する工業的にも有用かつ新規性のある学術研究として意義がある。また、未利用バイオマスであるリグニン由来の芳香族化合物に炭酸を固定化して芳香族カルボン酸を合成するバイオプロセスの導入は、脱炭素化社会の実現に大いに貢献するものである。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (24 results)

All 2021 2020 2019

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 2 results) Presentation (15 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Search for useful microbial enzymes and applied research for bioprocess development2021

    • Author(s)
      木野 邦器
    • Journal Title

      Seibutsu-kogaku Kaishi

      Volume: 99 Issue: 1 Pages: 2-14

    • DOI

      10.34565/seibutsukogaku.99.1_2

    • NAID

      130007976697

    • ISSN
      0919-3758, 2435-8630
    • Year and Date
      2021-01-25
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 2,5-Furandicarboxylic acid production from furfural by sequential biocatalytic reactions2021

    • Author(s)
      Kawanabe Kazuki、Aono Riku、Kino Kuniki
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: - Issue: 1 Pages: 18-24

    • DOI

      10.1016/j.jbiosc.2021.03.001

    • NAID

      40022629746

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic reactions and microorganisms producing the various isomers of hydroxyproline2020

    • Author(s)
      Hara Ryotaro、Kino Kuniki
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 104 Issue: 11 Pages: 4771-4779

    • DOI

      10.1007/s00253-020-10603-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficient and long-term vanillin production from 4-vinylguaiacol using immobilized whole cells expressing Cso2 protein2020

    • Author(s)
      Saito Tsubasa、Aono Riku、Furuya Toshiki、Kino Kuniki
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 130 Issue: 3 Pages: 260-264

    • DOI

      10.1016/j.jbiosc.2020.04.012

    • NAID

      40022351578

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Screening, gene cloning, and characterization of orsellinic acid decarboxylase from Arthrobacter sp. K8 for regio-selective carboxylation of resorcinol derivatives2020

    • Author(s)
      Kino Kuniki、Hirokawa Yasutaka、Gawasawa Ryo、Murase Ryota、Tsuchihashi Ryohei、Hara Ryotaro
    • Journal Title

      Journal of Biotechnology

      Volume: 323 Pages: 128-135

    • DOI

      10.1016/j.jbiotec.2020.08.011

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemoenzymatic oxygenation method for sesquiterupenoid synthesis based on Fe-chelate and ferric-chelate reductase2020

    • Author(s)
      Satoru Umezawa, Hiroko Akao, Mio Kubota and Kuniki Kino
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 84 Issue: 4 Pages: 780-788

    • DOI

      10.1080/09168451.2019.1707062

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ectoine hydroxylase displays selective trans-3-hydroxylation activity towards L-proline2019

    • Author(s)
      Hara R, Nishikawa T, Okuhara T, Koketsu K, Kino K
    • Journal Title

      Appl. Microbiol. Biotechnol.

      Volume: 103 Issue: 14 Pages: 5689-5698

    • DOI

      10.1007/s00253-019-09868-y

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of Gram-negative and Gram-positive bacteria capable of producing poceatannol from resveratrol2019

    • Author(s)
      Toshiki Furuya, Naoto Imaki, Kosuke Shigei, Masahiko Sai, and Kuniki Kino
    • Journal Title

      Appl. Microbiol. Biotechnol.

      Volume: 103 Pages: 5811-5820

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a synthesis method for ordor sesquiterupenoid, (-)-rotundone, using non-heme Fe2+ chelate catalyst and ferric-chelate reductase2019

    • Author(s)
      Satoru Umezawa, Shunsuke Konishi, and Kuniki Kino
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 83 Issue: 10 Pages: 1875-1883

    • DOI

      10.1080/09168451.2019.1625264

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] 有用微生物酵素の探索とバイオプロセス開発への応用研究2020

    • Author(s)
      木野 邦器
    • Organizer
      日本生物工学会Webシンポジウム2020
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 新規可逆的脱炭酸酵素4-hydroxyisophthalic acid decarboxylaseの諸性質解明2020

    • Author(s)
      吉原 朋矢,青野 陸,木野 邦器
    • Organizer
      日本農芸化学会2020年度福岡大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] バイオプロセスによるフルフラールからの2,5-フランジカルボン酸生産2020

    • Author(s)
      川鍋 一樹,青野 陸,木野 邦器
    • Organizer
      日本農芸化学会2020年度福岡大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Biocatalytic synthesis of piceatannol from reveratrol by Ensifer and Arthrobacter strains2019

    • Author(s)
      Toshiki Furuya, Masahiko Sai, and Kuniki Kino
    • Organizer
      Biotrans2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of synthesis methods of odor sesquiterpenoids using ferric-chelate reductase2019

    • Author(s)
      Satoru Umezawa and Kuniki Kino
    • Organizer
      Biotrans2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A novel reversible decarboxylase towards aromatic dicarboxylic acid2019

    • Author(s)
      Riku Aono, Tomoya Yoshihara, Hotaka Nishida and Kuniki Kino
    • Organizer
      Biotrans2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ジオキシゲナーゼを利用した2-phenyl ethanolからのポリフェノール類の合成2019

    • Author(s)
      松出めぐみ,岡本姫佳, 木野邦器
    • Organizer
      第21回生体触媒化学シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] ferric-chelate reductaseを利用した化学的酵素的手法によるセシキテルペノイド合成2019

    • Author(s)
      梅澤覚, 木野邦器
    • Organizer
      第21回生体触媒化学シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] trans-3-ヒドロキシプロリンの実用生産に向けたバイオプロセス開発2019

    • Author(s)
      原良太郎,西川健幸,奥原拓也,纐纈健人,木野邦器
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Fe2+-chelateおよびferric-chelate reductaseを組み合わせたセスキテルペノイド合成法の開発2019

    • Author(s)
      梅澤覚,木野邦器
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 4-メトキシサリチル酸を生成する6-メトキシサリチル酸脱炭酸酵素の発見2019

    • Author(s)
      青野陸,森弘樹,原良太郎,木野邦器
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] グラム陰性細菌およびグラム陽性細菌を用いたポリフェノールの生産2019

    • Author(s)
      青木瑠花, 重井宏介, 古屋俊樹, 木野邦器
    • Organizer
      酵素工学研究会第82回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ヒスチジンおよびグルタミンを基質とする新規水酸化酵素による反応生成物の絶対構造解析2019

    • Author(s)
      柳川洋晟,勝又智哉, 中島悠太, 原良太郎, 平沢泉, 木野邦器
    • Organizer
      酵素工学研究会第82回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 芳香族ジカルボン酸に対する新規可逆的脱炭酸酵素の探索2019

    • Author(s)
      西田穂高、青野陸、吉原朋矢、木野邦器
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 4-hydroxyisophthalic acidに対する新規可逆的脱炭酸酵素の同定と遺伝子解析2019

    • Author(s)
      吉原朋矢、青野陸、木野邦器
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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