| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Outline of Final Research Achievements |
In this study, we have developed an in-line force-curve mode, which is an advanced molecular manipulation function of high-speed atomic force microscopy (AFM), allowing us to form single tubulin dimer defects in microtubules with high controllability and to obtain force-distance curves in realtime. This technique enabled us to observe the self-healing process of structural defects created on a microtubule and the translational motility of kinesin molecules around the defects, and to quantify the binding energy between tubulins. Furthermore, we have extended this technique to the fast force mapping mode. Also, by observing the kinesin sliding motion in bent microtubules, we found that the kinesin translational velocity is significantly reduced in the bent region.
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