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Establishment of single biomolecule NMR observation method using fluorescent diamond

Research Project

Project/Area Number 18H01838
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 28040:Nanobioscience-related
Research InstitutionOsaka University

Principal Investigator

Harada Yoshie  大阪大学, 蛋白質研究所, 教授 (10202269)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywordsダイヤモンド / ナノダイヤモンド / 窒素-格子空隙中心 / 光検出磁気共鳴 / 生物物理
Outline of Final Research Achievements

The electron spin of the nitrogen vacancy center (NVC) existing inside the fluorescent nanodiamond (FND) has two quantum states of ms = 0 and degenerate ms = ± 1, and the magnetic resonance between these quantum states. The frequency (D) changes with temperature. That is, the temperature around the FND can be known by measuring the value of D with the optical-detected magnetic resonance microscope we have developed. In this study, we developed a method to measure the absolute temperature inside cells by utilizing the property that the temperature measurement ability of FND is not affected by the surrounding environment.

Academic Significance and Societal Importance of the Research Achievements

温度は、生体機能の維持や細胞内反応を包括的に制御している最も基本的な物理量である。細胞の機能を深く理解するためには、細胞内の生体分子の振る舞いやそれを支配する温度の解明が不可欠である。これまでに、温度感受性のポリマーや蛍光タンパク質、蛍光色素を用いて、細胞内の温度を計測する研究が行われてきた。しかし、いずれの方法も、温度の時間変化や墓所による温度の違いなどの検出には有効であるが、絶対温度を計測することは非常に困難であった。今回FNDが周囲の環境にロバストである性質を利用して絶対温度を計測することができるようになったことで、細胞機能への温度の影響を明らかにする研究の進展が期待できる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (38 results)

All 2021 2020 2019 2018 Other

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 6 results) Presentation (23 results) (of which Int'l Joint Research: 12 results,  Invited: 9 results) Remarks (3 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] In situ measurement of intracellular thermal conductivity using heater-thermometer hybrid diamond nanosensor2021

    • Author(s)
      Shingo Sotoma, Chongxia Zhong, James Chen Yong Kah, Hayato Yamashita, Taras Plakhotnik, Yoshie Harada, Madoka Suzuki
    • Journal Title

      Science Advances

      Volume: 7 Issue: 3

    • DOI

      10.1126/sciadv.abd7888

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Tracking the 3D Rotational Dynamics in Nanoscopic Biological Systems.2020

    • Author(s)
      Ryuji Igarashi, Takuma Sugi, Shingo Sotoma, Takuya Genjo, Yuta Kumiya, Erik Walinda, Hiroshi Ueno, Kazuhiro Ikeda, Hitoshi Sumiya, Hidehito Tochio, Yohsuke Yoshinari, Yoshie Harada, Masahiro Shirakawa
    • Journal Title

      Journal of the American Chemical Society

      Volume: 142 Issue: 16 Pages: 7542-7554

    • DOI

      10.1021/jacs.0c01191

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] HCV IRES captures an actively translating 80S ribosome2019

    • Author(s)
      Yokoyama Takeshi、Machida Kodai、Iwasaki Wakana、Shigeta Tomoaki、Nishimoto Madoka、Takahashi Mari、Sakamoto Ayako、Yonemochi Mayumi、Harada Yoshie、Shigematsu Hideki、Shirouzu Mikako、Tadakuma Hisashi、Imataka Hiroaki、Ito Takuhiro
    • Journal Title

      Molecular Cell

      Volume: 印刷中 Issue: 6 Pages: 1205-1214

    • DOI

      10.1016/j.molcel.2019.04.022

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Polydopamine Coating as a Scaffold for Ring-Opening Chemistry To Functionalize Gold Nanoparticles2019

    • Author(s)
      Sotoma Shingo、Harada Yoshie
    • Journal Title

      Langmuir

      Volume: 35 Pages: 8357-8362

    • DOI

      10.1021/acs.langmuir.9b00762

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formation of perfectly aligned high-density NV centers in (111) CVD-grown diamonds for magnetic field imaging of magnetic particles2019

    • Author(s)
      Ozawa Hayato、Hatano Yuji、Iwasaki Takayuki、Harada Yoshie、Hatano Mutsuko
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 58 Issue: SI Pages: SIIB26-SIIB26

    • DOI

      10.7567/1347-4065/ab203c

    • NAID

      210000156695

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multifunctional temozolomide-loaded lipid superparamagnetic nanovectors: dual targeting and disintegration of glioblastoma spheroids by synergic chemotherapy and hyperthermia treatment2019

    • Author(s)
      Marino Attilio、Camponovo Alice、Degl'Innocenti Andrea、Bartolucci Martina、Tapeinos Christos、Martinelli Chiara、De Pasquale Daniele、Santoro Francesca、Mollo Valentina、Arai Satoshi、Suzuki Madoka、Harada Yoshie、Petretto Andrea、Ciofani Gianni
    • Journal Title

      Nanoscale

      Volume: 11 Issue: 44 Pages: 21227-21248

    • DOI

      10.1039/c9nr07976a

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Enrichment of ODMR-active nitrogen-vacancy centres in five-nanometre-sized detonation- synthesized nanodiamonds: Nanoprobes for temperature, angle and position2018

    • Author(s)
      Sotoma S, Terada D, Segawa TF, Igarashi R, Harada Y, Shirakawa M.
    • Journal Title

      Sci.Rep.

      Volume: 8 Issue: 1 Pages: 5463-5463

    • DOI

      10.1038/s41598-018-23635-5

    • NAID

      120006517896

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] One-Pot Synthesis of Highly Dispersible Fluorescent Nanodiamonds for Bioconjugation2018

    • Author(s)
      Terada Daiki、Sotoma Shingo、Harada Yoshie、Igarashi Ryuji、Shirakawa Masahiro
    • Journal Title

      Bioconjugate Chemistry

      Volume: 29 Issue: 8 Pages: 2786-2792

    • DOI

      10.1021/acs.bioconjchem.8b00412

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescent nanodiamonds as a robust temperature sensor inside a single cell2018

    • Author(s)
      Takeharu Sekiguchi, Shingo Sotoma, Yoshie Harada
    • Journal Title

      Biophysics and Physicobiology

      Volume: 15 Issue: 0 Pages: 229-234

    • DOI

      10.2142/biophysico.15.0_229

    • NAID

      130007501130

    • ISSN
      2189-4779
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Magnetic Field Imaging of Super-paramagnetic Particles Using High-Density, Perfectly Oriented NV Centres in Diamond CVD Film2018

    • Author(s)
      Y. Hatano, T. Sekiguchi, T. Iwasaki, M. Hatano, and Y. Harada
    • Journal Title

      Phys. Status Solidi A

      Volume: 215 Issue: 22 Pages: 1800254-1800254

    • DOI

      10.1002/pssa.201800254

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] キネシン分子の空間配置が輸送複合体の運動に与える影響の評価2020

    • Author(s)
      福本 紘大, 宮薗 侑也, 多田隈 尚史, 原田 慶恵
    • Organizer
      日本生物物理学会第58回年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 神経分化における細胞内温度の関与2020

    • Author(s)
      中馬俊祐, 岡部弘基、原田 慶恵
    • Organizer
      日本生物物理学会第58回年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Intracellular thermometry with fluorescent polymer sensor and nanodiamond2020

    • Author(s)
      外間進悟、中馬俊祐, 、岡部弘基、原田 慶恵
    • Organizer
      ICCMSE 2020 Conference Quantum Science Symposium
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Involvement of intracellular temperature in neuronal differentiation2020

    • Author(s)
      中馬俊祐,、岡部弘基、原田 慶恵
    • Organizer
      第15回生命医科学研究所ネットワーク国際シンポジウム
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] DNAオリガミを用いたキネシン-1の空間配置が輸送複合体の協調運動に与える影響の解析2020

    • Author(s)
      福本 紘大, 宮薗 侑也, 多田隈 尚史, 原田 慶恵
    • Organizer
      日本分子生物学会第43回年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 高度に機能制御されたダイヤモンド量子センサによる細胞内センシングの実績2020

    • Author(s)
      外間進悟、仲崇霞、鈴木 団、張 煥正、原田 慶恵
    • Organizer
      量子生命科学会第2回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Dual-functionalized fluorescent nanodiamond as nanoheaters and nanothermometers in cells2020

    • Author(s)
      Chongxia Zhong, Shingo Sotoma, Taras Plakhotnik, James Chen Yong Kah, Yoshie Harada, Madoka Suzuki
    • Organizer
      2020 Annual Meeting - Biophysical Society
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 1分子イメージング顕微鏡によるタンパク質の機能解析2019

    • Author(s)
      原田慶恵
    • Organizer
      京都産業大学生命科学部バイオフォーラム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] NVセンターの生命科学計測への応用2019

    • Author(s)
      原田慶恵、外間進悟、波多野雄治、波多野睦子
    • Organizer
      量子生命科学研究会第1回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Fluorescent nanodiamond as a robust temperature sensor inside a single cell2019

    • Author(s)
      Yoshie Harada, Takeharu Sekiguchi, Shingo Sotoma
    • Organizer
      The third International Workshop on Symbiosis of Biology and Nanodevices
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a New Fluorescence Imaging Technique Using Nitrogen Vacancy Centers in Diamond2019

    • Author(s)
      Yoshie Harada
    • Organizer
      17th Chinese Biophysics Congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fluorescent nanodiamonds as a robust temperature sensor inside a single cell2019

    • Author(s)
      Shingo Sotoma, Yoshie Harada
    • Organizer
      E-MRS 2019 Fall Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fluorescent nanodiamonds for bioimaging applications2019

    • Author(s)
      Shingo Sotoma, Feng-Jen Hsieh, Huan-Cheng Chang, and Yoshie Harada
    • Organizer
      E-MRS 2019 Fall Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 磁性粒子とダイヤモンド基板中のNVセンタを用いた細胞計測2019

    • Author(s)
      波多野 雄治,多田隈 尚史,岩崎 孝之,波多野 睦子,原田 慶恵
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Application of individual fluorescent nanodiamond as nanothermometer and nanoheater2019

    • Author(s)
      Chongxia Zhong, Shingo Sotoma, Yoshie Harada, Madoka Suzuki
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Fluorescent Nanodiamonds as a Robust Temperature Sensor inside a Single Cell2019

    • Author(s)
      Yoshie Harada
    • Organizer
      Asian Chemical Biology Initiative 2019 Yangon Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fluorescent nanodiamond as a robust temperature sensor inside a single cell2019

    • Author(s)
      Yoshie Harada
    • Organizer
      The Second International Workshop by the 174th Committee JSPS on Symbiosis of Biology and Nanodevices
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Studies on Biomolecules Using Single-Molecule Imaging Technique2019

    • Author(s)
      Yoshie Harada
    • Organizer
      Deutsch-Japan Kolloquien
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Diamond Sensor - Device and System Construction2019

    • Author(s)
      Yuji Hatano, Hayato Ozawa, Takayuki Iwasaki, Mutsuko Hatano, Susumu Yasuda, Takeshi Ohshima, and Yoshie Harada
    • Organizer
      The 1st International Forum on Quantum Sensing (IFQS2019)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cell imaging with magnetic particle with on a diamond sensor2019

    • Author(s)
      Yoshie Harada, Takeharu Sekiguchi, Takayuki Iwasaki, Mutsuko Hatano, Yuji Hatano
    • Organizer
      9th FAOPS
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 高密度高配向NVセンタを含むダイヤモンドCVD膜によるバイオイメージング2018

    • Author(s)
      波多野雄治、関口武治、岩崎孝之、波多野睦子、原田慶恵
    • Organizer
      量子生命科学研究会・第2回学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] NVセンターの生命科学計測への適用2018

    • Author(s)
      原田慶恵、外間進悟、波多野雄治
    • Organizer
      第79回応用物理学会秋季学術講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] NVセンターの生命科学計測への適用2018

    • Author(s)
      原田慶恵
    • Organizer
      蛋白研セミナー:構造生物学と計算科学の融合による動的構造生物学の新しい展開
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Remarks] 大阪大学蛋白質研究所原田慶恵研研究概要

    • URL

      https://trais-out2.sakura.ne.jp/protein-nanobiology/research/

    • Related Report
      2020 Annual Research Report
  • [Remarks] 蛋白質ナノ科学研究室

    • URL

      http://www.protein.osaka-u.ac.jp/laboratories/nanobiology

    • Related Report
      2019 Annual Research Report
  • [Remarks] 大阪大学 蛋白質研究所 原田慶恵研 研究テーマ

    • URL

      https://www.ccc.osaka-u.ac.jp/protein/nanobiology/research/

    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] MAGNETIC MESURING DEVICE2021

    • Inventor(s)
      Hatano M et al.
    • Industrial Property Rights Holder
      Hatano M et al.
    • Industrial Property Rights Type
      特許
    • Filing Date
      2021
    • Related Report
      2020 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 磁気画像計測装置2019

    • Inventor(s)
      原田慶恵、波多野雄治、多田隈尚史、波多野睦子、岩崎孝之
    • Industrial Property Rights Holder
      原田慶恵、波多野雄治、多田隈尚史、波多野睦子、岩崎孝之
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-097873
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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