| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
Epidermal growth factor receptor (EGFR), which transmits primarily cell proliferation signals into cells after binding with an extracellular cytokine, concerns with critical diseases such as cancers. However, how EGFR regulates the cell responses through its molecular behavior remains unrevealed. In this study, we measured EGFR behaviors of both wild type and cancerous mutants by single-molecule imaging in cells and succeeded to detect changes in the molecular structures from the mobilities. Not only effects of anti-cancer drugs on EGFR but also the resistance of mutants against drugs were reflected in the behaviors. Furthermore, the activity of downstream signaling could be read out from the oligomerization of EGFR. The findings in the study will be a basis for a novel drug screening.
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