| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Outline of Final Research Achievements |
This study reports the detection and the identification of a single base from a single DNA oligomer by surface-enhanced Raman spectroscopy (SERS) using a gold nanoparticle dimer. The gold nanoparticle dimer structure was fabricated by a self-assembling process using a nanotrench. A Raman spectrum derived from an 8-chain DNA oligomer with one adenine and seven cytosines was obtained. It was confirmed that a single DNA oligomer was detected from a single gold nanoparticle dimer. It indicates that a single adenine was detected. In addition, only the adenine peak without the cytosine peak was observed, indicating the possibility of spatial resolution with a single DNA base.
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