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Development of single-molecule regulated chemistry by single molecule droplet array

Research Project

Project/Area Number 18H01848
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 28050:Nano/micro-systems-related
Research InstitutionOsaka Prefecture University

Principal Investigator

XU YAN  大阪府立大学, 工学(系)研究科(研究院), 准教授 (90593898)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2021: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywords1分子制御化学 / 1分子液滴 / ナノ流体デバイス / 1分子操作 / 1分子反応 / 液滴輸送 / 液滴融合 / Nano-in-Nano集積化
Outline of Final Research Achievements

In this study, we have developed fundamental mechanisms and technologies for manipulating femtoliter to attoliter (fL~aL) fluids using our nanofluidic device technology. Based on these developed mechanisms and technologies, we have achieved the fabrication of single-molecule droplet arrays. The use of the single-molecule droplet arrays has allowed the detection, manipulation, and reaction of single molecules at normal concentration range. Through this study, we have established the basic processes for "single molecule regulated chemistry" proposed by us.

Academic Significance and Societal Importance of the Research Achievements

本研究で確立した1分子制御化学の基本プロセスは化学やバイオの基礎と応用に関連する幅広い分野を革新する可能性がある。例えば、溶液反応の分子ダイナミクス解明や、集団平均化により隠蔽された個々の生体分子の挙動と特性の多様性究明、自然状態の長鎖DNAの任意合成やポリマー構造の自由構築に繋がる分子を積み木とする究極の精密合成法の創出などが期待される。従って、本研究成果は、1分子化学や1分子生物学、化学工学、プロセス工学、合成化学、合成生物学、定量生物学、分子診断、薬物スクリーニング、創薬など幅広い領域への学術的・科学技術的・社会的波及効果は極めて大きい。

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (65 results)

All 2021 2020 2019 2018 Other

All Journal Article (7 results) (of which Peer Reviewed: 6 results) Presentation (54 results) (of which Int'l Joint Research: 39 results,  Invited: 33 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Generation of Nanometer-Scale Geometrical Gas-Liquid Interfaces in Hydrophilic/Hydrophobic Patterned Nanochannels.2020

    • Author(s)
      Hiroto Kawagishi, Shuichi Kawamata, Yan Xu
    • Journal Title

      Proceedings of Micro Total Analysis Systems

      Volume: 1 Pages: 190-191

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication of Sub-40-Nanometer Nano-in-Nano Structures in Nanofluidic Channels2020

    • Author(s)
      Hiroki Kamai, Yan Xu
    • Journal Title

      Proceedings of Micro Total Analysis Systems

      Volume: 1 Pages: 366-367

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 1分子制御化学に向けたアトリットル液滴のアレイ化と混合2020

    • Author(s)
      川岸啓人, 許岩
    • Journal Title

      化学とマイクロ・ナノシステム

      Volume: 19.1 Pages: 29-30

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Nanofluidic fabrication and manipulation of attoliter droplets.2019

    • Author(s)
      H. Kawagishi, S. Kawamata, Y. Xu.
    • Journal Title

      Proceedings of Micro Total Analysis Systems 2019

      Volume: なし Pages: 117-118

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication and evaluation of attoliter droplets.2019

    • Author(s)
      R.Takane, H.Kawagishi, Y. Matsui, H.Ikeda, Y. Xu
    • Journal Title

      Proceedings of Micro Total Analysis Systems 2019

      Volume: なし Pages: 991-992

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication and evaluation of flexible nanovalves in 2d-nanochannels.2019

    • Author(s)
      H. Kawagishi, S. Funano, Y. Tanaka, S. Kawamata, Y. Xu
    • Journal Title

      Proceedings of Micro Total Analysis Systems 2019

      Volume: なし Pages: 1182-1183

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication of attoliter droplets by hydrophilic/hydrophobic nano-in-nano integrated structures.2018

    • Author(s)
      H. Kawagishi, S. Kawamata, Y. Xu.
    • Journal Title

      Proceedings of Micro Total Analysis Systems

      Volume: 0 Pages: 381-382

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Nanofluidics for New Chemistry, Biology, and Materials Science(Tentative)2020

    • Author(s)
      許岩
    • Organizer
      第137回分析技術研究会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] ナノ流路内親水/疎水ナノパターンによる気液ナノ界面の形成と応用2020

    • Author(s)
      川岸啓人, 川又修一, 許岩
    • Organizer
      化学とマイクロ・ナノシステム学会第42回研究会, CHEMINAS42th
    • Related Report
      2020 Annual Research Report
  • [Presentation] Generation of Nanometer-Scale Geometrical Gas-Liquid Interfaces in Hydrophilic/Hydrophobic Patterned Nanochannels.2020

    • Author(s)
      Hiroto Kawagishi, Shuichi Kawamata, Yan Xu
    • Organizer
      The 24rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, (MicroTAS2020)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication of Sub-40-Nanometer Nano-in-Nano Structures in Nanofluidic Channels2020

    • Author(s)
      Hiroki Kamai, Yan Xu
    • Organizer
      The 24rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, (MicroTAS2020)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 超微細Nano-in-Nano構造を駆使したNanofuidic SERS検出2020

    • Author(s)
      釜井弘樹, 許岩
    • Organizer
      化学とマイクロ・ナノシステム学会第42回研究会, CHEMINAS42th
    • Related Report
      2020 Annual Research Report
  • [Presentation] 1分子ダイナミクス解明に向けたaL液滴アレイの作製と条件検討.2019

    • Author(s)
      高根里咲, 川岸啓人, 許岩
    • Organizer
      化学とマイクロ・ナノシステム学会第39回研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 1分子制御化学に向けたアトリットル液滴の形成と移動.2019

    • Author(s)
      川岸啓人, 川又修一, 許岩
    • Organizer
      化学とマイクロ・ナノシステム学会第39回研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Fabrication and evaluation of mechanical nanovalves in nanochannels.2019

    • Author(s)
      H. Kawagishi, S. Funano, Y. Tanaka, S. Kawamata, Y. Xu
    • Organizer
      The 11th International Symposium on Microchemistry and Microsystems (ISMM 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 1分子制御化学に向けたアトリットル 液滴のマニピュレーションと融合.2019

    • Author(s)
      川岸啓人, 川又修一, 許岩
    • Organizer
      日本分析化学会第68年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ナノ流体デバイスにおける光圧によるナ ノ粒子間反応ダイナミクスの解析.2019

    • Author(s)
      西岡賢史, 許岩
    • Organizer
      日本分析化学会第68年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 局所的に構築したナノ流路内蒸気圧差による気-液ナノ界面の形成と応用.2019

    • Author(s)
      川岸啓人, 川又修一, 許岩
    • Organizer
      日本分析化学会第68年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Nanofluidic fabrication and manipulation of attoliter droplets.2019

    • Author(s)
      H. Kawagishi, S. Kawamata, Y. Xu
    • Organizer
      The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2019),
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication and evaluation of attoliter droplets.2019

    • Author(s)
      R.Takane, H.Kawagishi, Y.Matsui, H.Ikeda, Y. Xu
    • Organizer
      The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2019),
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication and evaluation of flexible nanovalves in 2D-nanochannels.2019

    • Author(s)
      H. Kawagishi, S. Funano, Y. Tanaka, S. Kawamata, Y. Xu
    • Organizer
      The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2019),
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 1分子制御化学に向けたアトリットル液滴のアレイ化と混合.2019

    • Author(s)
      川岸啓人, 許岩
    • Organizer
      化学とマイクロ・ナノシステム学会第40回研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] What can we expect with nanofluidics. An exciting arena for new chemistry, biology, and materials science.2019

    • Author(s)
      Y.Xu
    • Organizer
      The 11th International Symposium on Microchemistry and Microsystems (ISMM2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering nanofludics for new chemistry, biology, and materials science.2019

    • Author(s)
      許岩
    • Organizer
      奈良先端科学技術大学院大学光ナノサイエンス特別講義
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Beyond Phenomena: Pioneering Applied Nanofluidics2019

    • Author(s)
      Y.Xu
    • Organizer
      Shanghai University of Traditional Chinese Medicine
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering nanofludics for new chemistry,biology,and materials science.2019

    • Author(s)
      Y.Xu
    • Organizer
      Invited Talk at Zhejiang Univeristy
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2019

    • Author(s)
      Y.Xu
    • Organizer
      Invited Talk at Northeastern University
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering nanofludics for new chemistry,biology,and materials science.2019

    • Author(s)
      Y.Xu
    • Organizer
      DICP 70th Anniversary Forum
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2019

    • Author(s)
      Y.Xu
    • Organizer
      IInvited Talk at nternational Iberian Nanotechnology Laboratory (INL)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2019

    • Author(s)
      Y.Xu
    • Organizer
      Invited Talk at Changzhou University
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Towards molecular manual-assembly: nanofluidic manipulation of single nanometric objects and extracellular vesicles.2019

    • Author(s)
      Y. Xu
    • Organizer
      The 2nd Symposium for Cell Analysis on Micro/Nanofluidics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nanofluidics for New Chemistry, Biology, and Materials Science.2019

    • Author(s)
      Y.Xu
    • Organizer
      Invited Seminar at The University of Tokyo
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2019

    • Author(s)
      Y.Xu
    • Organizer
      Invited Talk at Beijing Advanced Innovation Center for Biomedical Engineering
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2019

    • Author(s)
      Y. Xu
    • Organizer
      Invited Talk at The BME Seminar of Pennsylvania State University
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering and Advancing Nanofluidics for New Chemistry, Biology, and Materials Science.2019

    • Author(s)
      Y. Xu
    • Organizer
      Invited Talk at The BME colloquium of New York University (NYU)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering nanofluidics for new chemistry, biology, and materials science.2019

    • Author(s)
      Y. Xu
    • Organizer
      BDR Symposium 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nanofluidics for NanoBioChem Integration at Femtoliter, Attoliter, and Single-Molecule Scales.2018

    • Author(s)
      Y. Xu
    • Organizer
      The 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nanofluidics: Pioneering NanoBioChem Integration at Femtoliter, Attoliter, Single-Nanoparticle, and Single-Molecule Scales.2018

    • Author(s)
      Y. Xu
    • Organizer
      BIGHEART Lecture of National University of Singapore
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fabrication of attoliter droplets in hydrophobic nanochannels with hydrophilic nanopatterns.2018

    • Author(s)
      H. Kawagishi, S. Kawamata, Y. Xu,
    • Organizer
      The 10th International Symposium on Microchemistry and Microsystems (ISMM 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cutting of nanofluids using difference of vapor pressure in nanochannels.2018

    • Author(s)
      H. Kawagishi, S. Kawamata, Y. Xu
    • Organizer
      The 10th International Symposium on Microchemistry and Microsystems (ISMM 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2018

    • Author(s)
      Y. Xu
    • Organizer
      Invited Talk at Peking University
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] NanoBioChem Integration at Femtoliter, Attoliter, and Single-Molecule Scales through Nanofluidics.2018

    • Author(s)
      Y. Xu
    • Organizer
      Microsystems & Nanoengineering Summit 2018(MINE 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nanofluidics for NanoBioChem Integration at Femtoliter, Attoliter, and Single-Molecule Scales.2018

    • Author(s)
      Y. Xu
    • Organizer
      Invited Talk at Beijing University of Chemical Technology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Beyond Phenomena: Pioneering Applications of Nanofluidics through Nano-in-Nano Integration.2018

    • Author(s)
      Y. Xu
    • Organizer
      The 9th Japan-China-Korea Joint Conference on MEMS/NMES (JCK MEMS/NEMS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2018

    • Author(s)
      Y. Xu
    • Organizer
      Outstanding Alumni Lecture of Dalian Insititute of Chemical Physics (DICP) Chinese Academy of Sciences(CAS)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2018

    • Author(s)
      Y. Xu
    • Organizer
      Invited Talk at Tongji University
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2018

    • Author(s)
      Y. Xu
    • Organizer
      Invited Talk at Lanzhou University
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2018

    • Author(s)
      Y. Xu
    • Organizer
      Invited Talk at Taiyuan University of Technology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2018

    • Author(s)
      Y. Xu
    • Organizer
      Invited Talk at Shanxi University
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2018

    • Author(s)
      Y. Xu
    • Organizer
      Invited Talk at Northwest A&F University
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry Biology and Materials Science.2018

    • Author(s)
      Y. Xu
    • Organizer
      The First symposium for Cell Analysis on Micro/Nanofluidics
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nanofluidics: A New Arena for Chemistry, Biology, and Materials Science.2018

    • Author(s)
      Y. Xu
    • Organizer
      20th Annual Conference of the Chinese Society of Micro-Nano Technology 9th International Conference of the Chinese Society of Micro-Nano Technology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2018

    • Author(s)
      Y. Xu
    • Organizer
      The 3rd International Conference on Emerging Advanced Nanomaterials (ICEAN 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2018

    • Author(s)
      Y. Xu
    • Organizer
      Queensland Micro- and Nanotechnology Centre (QMNC) Seminar of Griffith University
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fabrication of attoliter droplets by hydrophilic/ hydrophobic nano-in-nano integrated structures.2018

    • Author(s)
      H. Kawagishi, S. Kawamata, Y. Xu
    • Organizer
      The 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Pioneering Nanofluidics for New Chemistry, Biology, and Materials Science.2018

    • Author(s)
      Y. Xu
    • Organizer
      The 12th NanoSquare Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication of single molecule droplets for elucidation of single molecule dynamics.2018

    • Author(s)
      R. Takane, H. Kawagishi, Y. Matsui, H. Ikeda, Y. Xu
    • Organizer
      The 12th NanoSquare Workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nanofluidics for NanoBioChem Integration at Femtoliter, Attoliter, and Single-Molecule Scales2018

    • Author(s)
      許岩
    • Organizer
      平成30年度 生理研研究会「生体コモンスペース研究会」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 1分子ダイナミクス解明に向けた1分子液滴の作製2018

    • Author(s)
      高根里咲, 川岸啓人, 松井康哲, 池田浩, 許岩
    • Organizer
      化学とマイクロ・ナノシステム学会第38回研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Nano-in-Nano集積化とフレキシブルなガラスによるナノ流路内バルブの作製2018

    • Author(s)
      川岸啓人, 船野俊一, 田中陽, 川又修一, 許岩
    • Organizer
      化学とマイクロ・ナノシステム学会第38回研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 1分子制御化学へ向けた親水/疎水ナノパターニング法による1分子液滴の大量生成2018

    • Author(s)
      川岸啓人, 川又修一, 許岩
    • Organizer
      RIMEDシーズ発掘・人材育成講演会
    • Related Report
      2018 Annual Research Report
  • [Book] Some frontier technologies for aptamers in medical applications in 'Aptamers for Medical Applications. From Diagnosis to Therapeutics' Pages 375-4032021

    • Author(s)
      Dong Y., Wang S., Zhao L., Yang J., Xu Y.
    • Total Pages
      475
    • Publisher
      Springer Nature
    • ISBN
      9789813348370
    • Related Report
      2020 Annual Research Report
  • [Book] Functional coatings for lab-on-a-chip systems based on phospholipid polymers, in 'Handbook of Modern Coating Technologies. Applications and Development ', pp555-5952021

    • Author(s)
      Xu Y., Takai M., Ishihara K.
    • Total Pages
      636
    • Publisher
      Elsevier
    • ISBN
      9780444632395
    • Related Report
      2020 Annual Research Report
  • [Book] Beyond Phenomena: Functionalization of Nanofluidics Based on Nano-in-Nano Integration Technology, in ‘21st Century Nanoscience - A Handbook, Exotic Nanostructures and Quantum Systems (Volume Five)’, pp13-1~13-182020

    • Author(s)
      Xu Y.
    • Total Pages
      488
    • Publisher
      Taylor & Francis (CRC Press)
    • ISBN
      9780815356264
    • Related Report
      2020 Annual Research Report
  • [Remarks] 許研究室HP

    • URL

      http://www.chemeng.osakafu-u.ac.jp/group8/index.html

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report 2018 Annual Research Report

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Published: 2018-04-23   Modified: 2023-03-23  

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