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Mechanical diagnosis and assay of cancer cells based on cell ergodicity

Research Project

Project/Area Number 18H01850
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 29010:Applied physical properties-related
Research InstitutionHokkaido University

Principal Investigator

OKAJIMA TAKAHARU  北海道大学, 情報科学研究院, 教授 (70280998)

Co-Investigator(Kenkyū-buntansha) 末岡 和久  北海道大学, 情報科学研究院, 教授 (60250479)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywords原子間力顕微鏡 / 細胞メカニクス / がん細胞 / 細胞力学診断 / 細胞力学 / 細胞診断 / 細胞アッセイ / エルゴード性
Outline of Final Research Achievements

We studied a platform of atomic force microscopy (AFM)-based system that diagnoses normal and cancerous cells at the single cell level. We developed micro-fabricated substrates for patterning single cells and an AFM system allowing a large number of cells adhered on micro-patterned substrates. Using normal and pre-cancer cells, we found that the classification accuracy is highly dependent on the micro-patterned geometry and the measurement locations within the patterned cells. Furthermore, we found that the shape (height) of micro-patterned cells is also a useful indicator for single cell mechanical diagnosis. In addition, we found that AFM had a potential to map the intracellular structures at their characteristic time scales.

Academic Significance and Societal Importance of the Research Achievements

がん細胞を1細胞単位で診断する技術は、究極のがん早期発見技術と言っても過言ではない。本研究では、原子間力顕微鏡(AFM)を用いて、がんの進行度を1細胞単位で力学的に同定する新手法を開発した。各種細胞の形態制御が可能なマイクロパターン基板と細胞力学特性の精密計測法を駆使して、1細胞レオロジーの網羅計測が可能な広範囲計測が可能なAFMシステムを開発した。本研究から、原子間力顕微鏡を用いた時間空間細胞力学計測によりがん細胞の診断が可能になった。今後は、細胞力学診断の高速化や様々な部位のがんへの適用およびアッセイの研究を開拓する必要がある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (30 results)

All 2021 2020 2019 2018

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (23 results) (of which Int'l Joint Research: 7 results,  Invited: 9 results) Book (3 results)

  • [Journal Article] Spatiotemporal dynamics of single cell stiffness in the early developing ascidian chordate embryo2021

    • Author(s)
      Fujii Yuki、Koizumi Wataru C.、Imai Taichi、Yokobori Megumi、Matsuo Tomohiro、Oka Kotaro、Hotta Kohji、Okajima Takaharu
    • Journal Title

      Communications Biology

      Volume: 4 Issue: 1

    • DOI

      10.1038/s42003-021-01869-w

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Measuring viscoelasticity of soft biological samples using atomic force microscopy2020

    • Author(s)
      Y. M. Efremov, T. Okajima, and A. Raman,
    • Journal Title

      Soft Matter

      Volume: 16 Issue: 1 Pages: 64-81

    • DOI

      10.1039/c9sm01020c

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Spontaneous Spatial Correlation of Elastic Modulus in Jammed Epithelial Monolayers Observed by AFM2019

    • Author(s)
      Y. Fujii, Y. Ochi, M. Tuchiya, M. Kajita, Y. Fujita, Y. Ishimoto, T. Okajima
    • Journal Title

      Biophysical Journal

      Volume: 116 Issue: 6 Pages: 1152-1158

    • DOI

      10.1016/j.bpj.2019.01.037

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Calibrating the Young’s modulus of soft materials with surface tilt angle measured by atomic force microscopy2019

    • Author(s)
      Y. Fujii, T. Okajima
    • Journal Title

      AIP Advances

      Volume: 9 Issue: 1 Pages: 015028-015028

    • DOI

      10.1063/1.5046372

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] AFMによる発生胚のメカニクス2021

    • Author(s)
      岡嶋孝治
    • Organizer
      2021年応物春季学術講演会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 原子間力顕微鏡:細胞・組織のメカニクス測定2021

    • Author(s)
      岡嶋孝治
    • Organizer
      日本薬剤学会・物性FGセミナー2020
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Intracellular structures and mechanical properties of cardiac fibroblasts from pediatric patients with restrictive cardiomyopathy2020

    • Author(s)
      M.Matsumoto, H.Suginobe, H.Tsuru, J.Narita, S.Kogaki, H.Ishida, T.Okajima,
    • Organizer
      28th International Colloquium on Scanning Probe Microscopy (ICSPM28)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Measuring Mechanical Properties of Developing Embryo with Tandemly Arranged Colloidal Probe AFM2020

    • Author(s)
      Y.Fujii, T.Matsuo, T.Okajima
    • Organizer
      28th International Colloquium on Scanning Probe Microscopy (ICSPM28)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 原子間力顕微鏡を用いた初期発生胚の時空間メカニクスーAFMを用いた発生胚メカニクス計測法2020

    • Author(s)
      岡嶋孝治
    • Organizer
      第5回ホヤ研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Nanomechanics of cell and tissue probed by atomic force microscopy2020

    • Author(s)
      T. Okajima
    • Organizer
      6th International Conference on Electronic Materials and Nanotechnology for Green Environment (ENGE 2020)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] AFMによる細胞・組織の力学情報の定量化2020

    • Author(s)
      岡嶋孝治
    • Organizer
      2020年第81回応用物理学会秋季学術講演会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] バイオAFM計測:1細胞から多細胞へ2020

    • Author(s)
      岡嶋孝治
    • Organizer
      第59回日本生体医工学会大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 原子間力顕微鏡による細胞・組織メカニクスの定量化2020

    • Author(s)
      岡嶋孝治
    • Organizer
      第67回応用物理学会春期学術講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Elasticity of Brewery Yeast under Ethanol Stress Investigated by Atomic Force Microscopy2019

    • Author(s)
      K.Toyota, R.Tanaka, T.Okajima
    • Organizer
      27th International Colloquium on Scanning Probe Microscopy (ICSPM27)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Relationship between Rheological Properties and Actin Density in Single Cells Investigated by AFM2019

    • Author(s)
      R.Tanaka, M.Sawano, K.Kuribayashi-Shigetomi, T.Okajima,
    • Organizer
      27th International Colloquium on Scanning Probe Microscopy (ICSPM27)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mechanics of cardiac fibroblasts from child patients with restrictive cardiomyopathy investigated by atomic force microscopy2019

    • Author(s)
      M.Matsumoto, H.Suginobe, S.Katsuragi, H.Tsuru, J.Narita, S.Kogaki, H.Ishida, T.Okajima、
    • Organizer
      The 3rd JCS Council Forum on Basic Cardiac Vascular Research
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロパターン基板上に形成する微小腫瘍のcell-in-cell構造の観察2019

    • Author(s)
      伊藤一馬、繁富(栗林)香織、宮武由甲子、スバギョ・アグス、末岡和久、岡嶋孝治
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 原子間力顕微鏡による単一がん細胞診断能の評価:細胞形状依存性2019

    • Author(s)
      小倉花歩、繁富(栗林)香織、スバギョ・アグス、末岡和久、岡嶋孝治、
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 分岐形態形成過程の組織力学測定のための原子間力顕微鏡2019

    • Author(s)
      関玲央捺、杉本健太、岡嶋 孝治、
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 多細胞系力学測定のための原子間力顕微鏡の開発2019

    • Author(s)
      土生飛翔、田中良昌、藤井裕紀、岡嶋 孝治
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 癌腫瘍組織のダイナミクス観察のためのマイクロ・ナノパターン基板2019

    • Author(s)
      繁富香織、宮武由甲子太田悠介、アグス・スバギョ、末岡和久、笠原正典、岡嶋孝治
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] AFMよる1細胞・細胞集団のナノメカニクス2019

    • Author(s)
      岡嶋孝治
    • Organizer
      日本顕微鏡学会第75回学術講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] AFMによる1細胞・多細胞系のナノ力学計測2019

    • Author(s)
      岡嶋孝治
    • Organizer
      2019年日本表面真空学会学術講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] AFMによる多細胞系のメカニクス2019

    • Author(s)
      岡嶋孝治
    • Organizer
      日本顕微鏡学会 ソフトマテリアル研究部会 第6回講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 原子間力顕微鏡による単一がん細胞力学診断:細胞形状依存性2019

    • Author(s)
      小倉花歩、田中良昌、繁富(栗林)香織、スバギョアグス、末岡和久、岡嶋孝治
    • Organizer
      第66回応用物理学会春季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Single Cancer Cell Diagnosis Based on Power-Law Rheology by AFM2018

    • Author(s)
      T. Okajima, R. Tanaka, M. Nagase, K. Ogura, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka
    • Organizer
      ACSIN-14&ICSPM26
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 原子間力顕微鏡測定による細胞形状・細胞レオロジー変数を用いた単一がん細胞診断法の評価2018

    • Author(s)
      田中良昌、永瀬萌、繁富(栗林)香織、スバギョ・アグス、末岡和久、岡嶋孝治
    • Organizer
      第79回応用物理学会秋季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Book] ナノ計測2020

    • Author(s)
      重川 秀実、吉村雅満、目良 裕、岡嶋 孝治
    • Total Pages
      192
    • Publisher
      近代科学社
    • ISBN
      9784764950290
    • Related Report
      2020 Annual Research Report
  • [Book] 生体の科学 70巻4号2019

    • Author(s)
      藤井裕紀、岡嶋孝治
    • Total Pages
      6
    • Publisher
      医学書院
    • Related Report
      2019 Annual Research Report
  • [Book] 高分子2019

    • Author(s)
      藤井裕紀、岡嶋孝治
    • Total Pages
      2
    • Publisher
      高分子学会
    • Related Report
      2019 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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