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Establishment of the new observation method for bioparticles using an SEM based on the elucidation of the properties of the NanoSuit.

Research Project

Project/Area Number 18H01869
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 29020:Thin film/surface and interfacial physical properties-related
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

HARIYAMA Takahiko  浜松医科大学, 光尖端医学教育研究センター, 特命研究教授 (30165039)

Co-Investigator(Kenkyū-buntansha) 鈴木 浩司  浜松医科大学, 医学部, 教務員 (50345831)
平川 聡史  浜松医科大学, 光尖端医学教育研究センター, 特任研究員 (50419511)
高久 康春  浜松医科大学, 光尖端医学教育研究センター, 特任研究員 (60378700)
河崎 秀陽  浜松医科大学, 光尖端医学教育研究センター, 准教授 (90397381)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords電子顕微鏡 / ナノ薄膜 / NanoSuit法 / 生体微粒子 / 表面科学 / NanoSuit_イムノクロマト法
Outline of Final Research Achievements

We have established the NanoSuit technology which can observe the alive/wet biological samples in an electron microscope which requires a high vacuum environment. The NanoSuit membrane can form on a biological sample surface by a quick one pot treatment. The membrane can protect biological samples in high vacuum, and add the conductivity against electron beam irradiation which usually causes the electric charge. By modifying the NanoSuit solution and the polymerization method, it became possible to observe not only cells and tissues but also industrial products.
We also succeeded to observe biological particles such as exosomes and viruses using the NanoSuit method. In the analysis using viruses, we succeeded in increasing the sensitivity by counting Au and/or Pt particles as the marker using the immunochromatography method when we cover the cellulose membrane of immunochromatography with NanoSuit membrane, therefore we could establish the new observation method for bioparticle.

Academic Significance and Societal Importance of the Research Achievements

エクソゾームやウイルスなどの生物関連微粒子を、走査型電子顕微鏡を用いて簡便に観察することは困難であった。NanoSuit法を改変して、この問題を解決する過程で、現行のイムノクロマト法による抗原抗体反応のマーカーの金属粒子をカウンティングすることを可能とした。その結果、新型コロナウイルス検出をRT-PCR検出法に匹敵する高感度化につなげることに成功した。本法は、多様な病原体や生体反応物質の検出に適用することができ、ヒトや家畜の健康管理に利用できることになった。動物からヒトへのウイルス感染など、今後引き起こされるだろうパンデミックの危機にも備えることができる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (21 results)

All 2021 2020 2019 2018

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 8 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 7 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] ナノスーツ法による医療診断への応用2021

    • Author(s)
      河崎秀陽
    • Journal Title

      医学のあゆみ

      Volume: 276 Pages: 740-748

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Living Organisms under an Electron Microscope: the NanoSuit® Method aiming for Medical and Industrial Applications2020

    • Author(s)
      Takahiko Hariyama, Yasuharu Takaku, Chiyo Senoh, Satoshi Yamada, Toshiya Itoh, Chiaki Suzuki, Sayuri Takehara, Satoshi Hirakawa, Hideya Kawasaki
    • Journal Title

      Journal of Photopolymer Science and Technology

      Volume: 33 Issue: 5 Pages: 517-522

    • DOI

      10.2494/photopolymer.33.517

    • NAID

      130007867678

    • ISSN
      0914-9244, 1349-6336
    • Year and Date
      2020-07-01
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Imaging dataset of fresh hydrous plants obtained by field-emission scanning electron microscopy conducted using a protective NanoSuit.2020

    • Author(s)
      Takehara S, Takaku Y(共筆頭著者), Shimomura M, Hariyama T
    • Journal Title

      PLOS ONE

      Volume: 15(5) Issue: 5 Pages: e0232992-e0232992

    • DOI

      10.1371/journal.pone.0232992

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sensitive quantitative and rapid immunochromatographic diagnosis of clinical samples by scanning electron microscopy - preparing for future outbreaks2020

    • Author(s)
      Kawasaki Hideya、Suzuki Hiromi、Maekawa Masato、Hariyama Takahiko
    • Journal Title

      medRxiv

      Volume: -

    • DOI

      10.1101/2020.05.20.20106864

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In situ elemental analysis of living biological specimens using ‘NanoSuit’ and EDS methods in FE-SEM2020

    • Author(s)
      Takaku Y, Takehara S, Suzuki C, Suzuki H, Shimomura M, Hariyama T
    • Journal Title

      Sci Rep

      Volume: 10(1) Issue: 1

    • DOI

      10.1038/s41598-020-71523-8

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Long interspersed nuclear element 1 hypomethylation has novel prognostic value and potential utility in liquid biopsy for oral cavity cancer2020

    • Author(s)
      Misawa Kiyoshi、Yamada Satoshi、Mima Masato、Nakagawa Takuya、Kurokawa Tomoya、Imai Atsushi、Mochizuki Daiki、Shinmura Daichi、Yamada Taiki、Kita Junya、Ishikawa Ryuji、Yamaguchi Yuki、Misawa Yuki、Kanazawa Takeharu、Kawasaki Hideya、Mineta Hiroyuki
    • Journal Title

      Biomarker Research

      Volume: 8 Pages: 113924-113924

    • DOI

      10.1016/j.jpba.2021.113924

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 新たな観察法としてのNanoSuit(R)法 ─ ウジ虫から学ぶ2020

    • Author(s)
      針山孝彦
    • Journal Title

      医学のあゆみ

      Volume: 274 Pages: 1136-1144

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 電子顕微鏡で観る昆虫: NanoSuit(R)法によるそのままの生物表面解析2020

    • Author(s)
      針山孝彦, 河崎秀陽, 高久康春
    • Journal Title

      昆蟲 ニューシリーズ

      Volume: 23 Pages: 145-155

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Living Organisms under an Electron Microscope: the NanoSuit<sup>®</sup>2019

    • Author(s)
      Hariyama Takahiko、Takaku Yasuharu、Suzuki Chiaki、Takehara Sayuri、Hirakawa Satoshi、Suzuki Hiroshi、Kawasaki Hideya
    • Journal Title

      Journal of Photopolymer Science and Technology

      Volume: 32 Issue: 2 Pages: 287-290

    • DOI

      10.2494/photopolymer.32.287

    • NAID

      130007744371

    • ISSN
      0914-9244, 1349-6336
    • Year and Date
      2019-06-24
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy2019

    • Author(s)
      Kawasaki Hideya、Itoh Toshiya、Takaku Yasuharu、Suzuki Hiroshi、Kosugi Isao、Meguro Shiori、Iwashita Toshihide、Hariyama Takahiko
    • Journal Title

      Laboratory Investigation

      Volume: 100 Issue: 1 Pages: 161-173

    • DOI

      10.1038/s41374-019-0309-7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Utility of Scanning Electron Microscopy Elemental Analysis Using the ‘NanoSuit’ Correlative Light and Electron Microscopy Method in the Diagnosis of Lanthanum Phosphate Deposition in the Esophagogastroduodenal Mucosa2019

    • Author(s)
      Shinmura Kazuya、Kawasaki Hideya、Baba Satoshi、Ohta Isao、Kato Hisami、Yasuda Hideo、Yamada Satoshi、Misawa Kiyoshi、Sugimoto Ken、Osawa Satoshi、Sato Masanori、Hariyama Takahiko、Sugimura Haruhiko
    • Journal Title

      Diagnostics

      Volume: 10 Issue: 1 Pages: 1-1

    • DOI

      10.3390/diagnostics10010001

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 異分野の知の融合によるナノスーツ電子顕微鏡観察法の発見、そして医療応用2020

    • Author(s)
      針山孝彦
    • Organizer
      第66回日本病理学会秋期特別総会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] NanoSuitから蟲瞰学まで-バイオミメティクスの展開2020

    • Author(s)
      針山孝彦
    • Organizer
      生物科学学会連合公開シンポジウム
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Living organisms under an electron microscope: the NanoSuit2019

    • Author(s)
      Hariyama T, Kawasaki H, Suzuki H, Takaku Y, Suzuki C, Takehara S
    • Organizer
      The 36th International Conference of Photopolymer Science and Technology (ICPST-36)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ナノスーツによる生物試料の生きたままの電子顕微鏡観察2019

    • Author(s)
      針山孝彦, 高久康春, 鈴木浩司, 平川聡史, 河崎秀陽
    • Organizer
      第68回高分子学会年次大会特別セッション
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 界面の修飾によって高真空内で生命維持を可能に-NanoSuit法による電子顕微鏡生態観察2019

    • Author(s)
      針山孝彦
    • Organizer
      粉体工学会2019年第2回ソフト粒子・界面研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 電子顕微鏡を用いた生きたままの生物表面微細構造解析-NanoSuit法の応用展開-2019

    • Author(s)
      針山孝彦, 高久康春, 河崎秀陽
    • Organizer
      19-2 バイオミメティクス研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ナノスーツ観察法の発見とその医療応用について~異分野の知の融合による生物模倣と、ベンチャー立ち上げ~2019

    • Author(s)
      針山孝彦
    • Organizer
      応用物理学会関西支部2019年度第2回支部講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] NanoSuit-生物試料を生きたまま濡れたまま電子顕微鏡観察-2019

    • Author(s)
      針山孝彦
    • Organizer
      第36回 コロイド・界面技術シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Nanosuits, Imaging living organisms in the Scanning Electron Microscope2018

    • Author(s)
      Hariyama T
    • Organizer
      Japanese-German Symposium "Biomimetics: Learning from Nature for an Innovative Future"
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] イムノクロマトグラフィーによる測定方法、イムノクロマトグラフィー測定用補助液、イムノク口マトグラフィーチップ、及びイムノクロマトグラフィー測定キット2020

    • Inventor(s)
      河崎秀陽, 針山孝彦
    • Industrial Property Rights Holder
      浜松医科大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-079912
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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