From Static Image Diagnosis to Dynamic Image Diagnosis: Development of Super-resolution Live-cell Cytodiagnosis Using Nonlinear Raman Scattering
| Project/Area Number |
18H02000
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 34020:Analytical chemistry-related
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| Research Institution | Kyushu University (2020-2021)
University of Tsukuba (2018-2019) |
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Principal Investigator |
Kano Hideaki 九州大学, 理学研究院, 教授 (70334240)
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| Co-Investigator(Kenkyū-buntansha) |
野口 雅之 筑波大学, 医学医療系, 教授 (00198582)
猪子 誠人 愛知医科大学, 医学部, 講師 (30393127)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | ラマン / CARS / 生細胞 / 非線形光学 / 顕微鏡 / ラマン散乱 / 細胞診 / 分子イメージング |
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| Outline of Final Research Achievements |
In this study, coherent anti-Stokes Raman scattering (CARS) was used to develop a new cytological diagnosis system that enables non-staining visualization of intracellular organelles for dynamical image diagnosis of living cells. In the development of a new microscopic imaging system, we launched the world's fastest ultra-broadband multiplex CARS spectroscopic microscope. By combining a microscopic imaging system with a on-stage living-cell incubator, selective visualization of metabolites and their time-lapse measurement were realized. Concerning the studies on brown adipocytes, we showed that they have the ability to maintain constant intracellular unsaturated lipids (unsaturated lipid homeostasis).
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、coherent anti-Stokes Raman scattering (CARS）を基盤技術として用い、細胞内オルガネラをそのままライブで可視化する”動画診断”を可能とする、まったく新しい細胞診の開発を目的として研究を行った。本研究で開発した装置は細胞の活性を維持しながらラベルフリーで細胞内分子を特定できるため、疾患の早期診断などに利用可能であると期待される。
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Report
(4 results)
Research Products
(65 results)
