Two-dimensional super-thin layer chromatography / nano-structure assisted laser desorption ionization mass spectrometry using nanowire device
| Project/Area Number |
18H02009
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 34020:Analytical chemistry-related
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
安井 隆雄 名古屋大学, 工学研究科, 准教授 (00630584)
中嶋 秀 東京都立大学, 都市環境科学研究科, 准教授 (10432858)
手嶋 紀雄 愛知工業大学, 工学部, 教授 (30292501)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | ナノワイヤ / 酸化亜鉛 / 薄層クロマトグラフィー / レーザー脱離イオン化 / 質量分析 / 脂質 / マトリックスフリー / 超薄層クロマトグラフィー / MALDI / TLC |
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| Outline of Final Research Achievements |
Although lipidomics, the study of lipids, have a great potential as a clinical tool for monitoring metabolic changes in health and disease, the analytical techniques for lipids have not made substantial advances during the last decade. In order to promote lipidomics research, in the present project, we have undertaken the development of zinc oxide nanowire array-grown glass substrate plates, which have a dual role as a thin-layer chromatography (TLC) plate and a target plate for laser desorption/ionization (LDI) . We have refined hydrothermal synthesis technique and succeeded in producing a variety of nanowires of an intended size on a glass substrate. We have also developed an interface device for putting the UTLC plate into commercially available conventional MALDI-MS instrument.
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| Academic Significance and Societal Importance of the Research Achievements |
科学の進歩には革新的な分析法・分析技術の開発が不可欠である。本研究は、1細胞での分離分析を実現しうるポテンシャルのある分析法として学術的な意義は大きいと思われる。
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Report
(4 results)
Research Products
(52 results)
