| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Outline of Final Research Achievements |
We have developed facile enzymatic methods to synthesize DNA strands containing a ligand-type hydroxypyridone (H) nucleotide, which forms a Cu(II)-mediated base pair (H-Cu(II)-H). The H-modified oligonucleotides were synthesized by a primer extension reaction using two DNA polymerases or by a two-step reaction with polymerases and ligases. The enzymatic synthesis was subsequently applied to the development of metal-responsive allosteric DNAzymes, whose catalytic activity can be regulated by the formation of metal-mediated base pairs. The rational design strategy and the easy enzymatic synthesis established in this study provide a versatile way to develop a variety of metal-responsive DNA materials based on metal-mediated artificial base pairing.
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