| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Outline of Final Research Achievements |
Efficient hydrogen evolution from formate using biocatalysts is an attractive mean to produce an environmentally clean energy source. However, the biocatalysts for formate oxidation and hydrogen evolution are extremely oxygen-labile. We present an excellent model system for hydrogen evolution from formate using enzymatic reaction by the encapsulated cytoplasmic membrane of Citrobacter sp. S-77 containing O2-stable [NiFe]-hydrogenase and [Mo]-formate dehydrogenase immobilized on Ketjenblack EC-600JD with sodium alginate. The immobilized biocatalysts lead to stabilization of hydrogen production from formate. Notably, its catalytic H2 production showed a remarkable O2-stability, maintaining its catalytic activity even after air exposure.
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