| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Outline of Final Research Achievements |
We have developed an artificial RNA switch that can control the expression of a target protein by combining two of our key technologies, 1) synthetic RNA binding small molecular ligands that bind specifically to the target RNA and thereby induce the secondary and tertiary structure changes of the RNA (molecular glue for RNA), and 2) ribozyme whose structure and catalytic function is controlled by the molecular glue. We successfully created RNA switches that share the core operating system described above but exhibit different responses; OFF switch where gene expression levels are inhibited by the ligand, ON switch where expression levels are increased by the ligand, and ON/OFF reversibly switch where expression levels are controlled bidirectionally by light stimuli. These RNA switches are functional in human-cultured cell models.
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