A multidisciplinary research program to enable the discovery of inhibitors of colanic acid excretion in bacteria
| Project/Area Number |
18H02109
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 37030:Chemical biology-related
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | Antibacterial / Colanic acid / Wza / Single-molecule / single-molecule / antibacterial / single-moleule / colanic acid / inhibitor / Inhibitor / bacteria / excretion |
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| Outline of Final Research Achievements |
Three pathogenic bacterial strains have been cultured and stored. WzaCA is transporter of colonic acid. Genomic DNA extraction followed by polymerase chain reaction and cloning onto desired vectors has enabled the expression of WzaCA. Meanwhile, chemical strategies have been developed for efficient production of potential blockers. A LC-MS system equipped with autosampler, fraction collector, temperature controller and multiple detectors which include UV/Vis, 3D-field, refractive index, high-resolution mass, etc. has been established for fast and accurate access to various potential blockers. Lastly, the proposed microscopic system equipped with an ideal EMCCD camera has been set up. Further screening of blockers is currently ongoing. Once a desired blocker is identified in single-molecule electrical recordings, all sorts of biochemical, biophysical and biological tests that have been established will be carried out. Results will be published in due course.
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| Academic Significance and Societal Importance of the Research Achievements |
Antibacterial resistance is a global challenge. Novel antibacterial strategies and targets are urgently needed. Colanic acid (CA) is one of outermost and most essential outer bacterial matrices in the formation of most biofilms. Inhibition of CA export will help eradicate biofilms.
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Report
(4 results)
Research Products
(4 results)
