Elucidation and control of iron homeostasis based on iron-sensing technology using N-oxide chemistry
| Project/Area Number |
18H02110
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 37030:Chemical biology-related
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2020: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
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| Keywords | 鉄代謝 / スクリーニング / 蛍光プローブ / 鉄イオン / イメージング / 鉄 |
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| Outline of Final Research Achievements |
Currently, drugs used in clinical practice to treat iron overload and iron deficiency are limited to chelating agents and iron supplements, respectively. This is also the case in cellular experiments, where there are very few useful chemical tools for iron research, including bioactive compounds that inhibit or promote iron uptake and efflux and fluorescent iron probes. In this study, we improved the N-oxide chemistry-based Fe(II) detection technology and developed a highly sensitive fluorescent probe for Fe(II) detection and a high-throughput intracellular Fe(II) assay system. Furthermore, we conducted HTS of a compound library using the high-throughput intracellular iron divalent assay system and found a new bioactive compound that modulate iron homeostasis.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は、これまで検出することが困難であった細胞内鉄イオンを容易に可視化できるようにしたものであり、鉄研究において最大のネックであった鉄(II)イオンの選択的検出が可能になった。さらに、ハイスループットスクリーニングにより得た新たな鉄制御生理活性化合物は、鉄代謝を標的とした創薬シーズにもなりうることから、創薬研究の第一歩となる。
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Report
(4 results)
Research Products
(49 results)
