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Trial for the improvement of N use efficiency in photosynthesis and plant growth in rice: simultaneous enhancement of carbon fixation and regeneration of its substrate

Research Project

Project/Area Number 18H02111
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionIwate University

Principal Investigator

Suzuki Yuji  岩手大学, 農学部, 准教授 (80374974)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Keywords光合成 / 炭酸同化 / 遺伝子組換え / イネ
Outline of Final Research Achievements

Rubisco is a key enzyme for photosynthesis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme for supply of the substrate for Rubisco. In the present study, Rubisco and GAPDH were simulutaneously overproduced in rice to improve the photosynthetic capacity. However, improvement in the photosynthetic rates were not observed. Instead, the photosynthetic capacity at elevated CO2 conditions was slightly improved in rice plants with overproduction of GAPDH alone, although such improvement in photosynthesis was not sufficient for improvement in plant growth.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、光合成炭素代謝の改変により光合成能力を強化したという、貴重な研究例となった。また、本研究の成果は、今後のさらなる光合成能力強化に向けた方向性を考えるための重要な情報となるとともに、作製した遺伝子組換えイネは、光合成能力強化のための母材料となる。本研究をさらに発展させることで、イネの物質生産能力の強化、および、食糧供給の増加に向けた、基礎的な技術を得ることができる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (35 results)

All 2021 2020 2019 2018

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 6 results) Presentation (26 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Journal Article] Co-overproducing Rubisco and Rubisco activase enhances photosynthesis in the optimal temperature range in rice2021

    • Author(s)
      Suganami Mao、Suzuki Yuji、Tazoe Youshi、Yamori Wataru、Makino Amane
    • Journal Title

      Plant Physiology

      Volume: 185 Pages: 108-119

    • DOI

      10.1093/plphys/kiaa026

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Overproduction of chloroplast glyceraldehyde-3-phosphate dehydrogenase improves photosynthesis slightly under elevated [CO2] conditions in rice2021

    • Author(s)
      Suzuki Yuji、Ishiyama Keiki、Sugawara Misaki、Suzuki Yuka、Kondo Eri、Takegahara-Tamakawa Yuki、Yoon Dong-Kyung、Suganami Mao、Wada Shinya、Miyake Chikahiro、Makino Amane
    • Journal Title

      Plant and Cell Physiology

      Volume: 62 Issue: 1 Pages: 156-165

    • DOI

      10.1093/pcp/pcaa149

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Photochemistry of photosystems II and I in rice plants grown under different N levels at normal and high temperature2021

    • Author(s)
      Takahashi Yuki、Wada Shinya、Noguchi Ko、Miyake Chikahiro、Makino Amane、Suzuki Yuji
    • Journal Title

      Plant and Cell Physiology

      Volume: 62 Issue: 7 Pages: 1121-1130

    • DOI

      10.1093/pcp/pcab020

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of co-overproduction of Rubisco and chloroplast glyceraldehyde-3-phosphate dehydrogenase on photosynthesis in rice2021

    • Author(s)
      Suzuki Y*, Ishiyama K, Cho A, Takegahara-Tamakawa Y, Wada S, Miyake C and Makino A
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 67 Issue: 3 Pages: 1150-1162

    • DOI

      10.1080/00380768.2021.1915100

    • NAID

      210000158988

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of overexpression of the Rubisco small subunit gene under the control of the Rubisco activase promoter on Rubisco contents of rice leaves at different positions2020

    • Author(s)
      Kudo Natsumi、Mano Kazuhisa、Suganami Mao、Kondo Eri、Suzuki Yuji、Makino Amane
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 66 Issue: 4 Pages: 569-578

    • DOI

      10.1080/00380768.2020.1780898

    • NAID

      210000158370

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photorespiration coupled with CO2 assimilation protects photosystem I from photoinhibition under moderate poly(ethylene glycol)-Induced osmotic stress in rice2020

    • Author(s)
      Wada Shinya、Miyake Chikahiro、Makino Amane、Suzuki Yuji
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 1121-1121

    • DOI

      10.3389/fpls.2020.01121

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of Overproduction of Rubisco Activase on Rubisco Content in Transgenic Rice Grown at Different N Levels2020

    • Author(s)
      Suganami Mao、Suzuki Yuji、Kondo Eri、Nishida Shinji、Konno So、Makino Amane
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 5 Pages: 1626-1626

    • DOI

      10.3390/ijms21051626

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Responses of the Photosynthetic Electron Transport Reactions Stimulate the Oxidation of the Reaction Center Chlorophyll of Photosystem I, P700, under Drought and High Temperatures in Rice2019

    • Author(s)
      Wada Shinya、Takagi Daisuke、Miyake Chikahiro、Makino Amane、Suzuki Yuji
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 20 Issue: 9 Pages: 2068-2068

    • DOI

      10.3390/ijms20092068

    • NAID

      120006647359

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of co-overproduction of sedoheptulose-1,7-bisphosphatase and Rubisco on photosynthesis in rice2018

    • Author(s)
      Suzuki Yuji、Wada Shinya、Kondo Eri、Yamori Wataru、Makino Amane
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 65 Issue: 1 Pages: 36-40

    • DOI

      10.1080/00380768.2018.1530053

    • NAID

      210000012043

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] イネの不安定な気孔開閉によるCO2 固定反応効率の変動と光呼吸によるP700 酸化誘導2021

    • Author(s)
      古谷吏侑、牧野周、鈴木雄二、嶋川銀河、和田慎也、三宅親弘
    • Organizer
      日本植物生理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 葉緑体型グリセルアルデヒド-3-リン酸デヒドロゲナーゼの過剰発現がイネの光合成と個体生育に及ぼす影響2021

    • Author(s)
      鈴木雄二、石山敬貴、菅原水彩季、鈴木優佳、近藤依里、竹ケ原(玉川)夕紀、尹 棟敬、和田慎也、菅波眞央、三宅親弘、牧野 周
    • Organizer
      日本植物生理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 欠損GS3遺伝子により大粒性を有した準同質遺伝子系統ノトヒカリの作出とほ場での収量評価試験2020

    • Author(s)
      尹 棟敬、香川昴亮、石山敬貴、菅波眞央、前 忠彦、鈴木雄二、石田宏幸、牧野 周、小原実広
    • Organizer
      日本土壌肥料学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イネにおけるRubiscoとRubisco activaseの同時増強が光合成に与える影響2020

    • Author(s)
      菅波眞央、鈴木雄二、田副雄士、牧野 周
    • Organizer
      日本土壌肥料学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] カルビンサイクル酵素グリセルアルデヒド-3-リン酸デヒドロゲナーゼおよびトリオースリン酸イソメラーゼの遺伝子組換えがイネ葉の光合成に及ぼす影響2020

    • Author(s)
      鈴木雄二、石山敬貴、菅原水彩季、鈴木優佳、玉川夕紀、和田慎也、菅波眞央、近藤依里、三宅親弘、牧野 周
    • Organizer
      日本土壌肥料学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 遺伝子組換え植物専用隔離ほ場水田を用いたRubisco増強イネの収量評価試験2020

    • Author(s)
      尹 棟敬、香川昴亮、石山敬貴、菅波眞央、前 忠彦、鈴木雄二、石田宏幸、小原実広、牧野 周
    • Organizer
      日本土壌肥料学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 異なる窒素施肥量・生育温度がイネの光化学系IIおよびIの光化学反応に及ぼす影響2020

    • Author(s)
      高橋佑季、和田慎也、野口 航、三宅親弘、牧野周、鈴木雄二
    • Organizer
      日本土壌肥料学会東北支部大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 6種の針葉樹におけるRubisco比活性の評価2020

    • Author(s)
      澤口尚生、影山創大、宮澤真一、牧野 周、鈴木雄二
    • Organizer
      日本土壌肥料学会東北支部大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] RubiscoとRubisco activase の同時過剰生産がイネの光合成に与える影響2020

    • Author(s)
      菅波 眞央、鈴木 雄二、田副 雄士、牧野 周
    • Organizer
      日本植物生理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 遺伝子組換え作物隔離ほ場におけるRubisco過剰生産イネの4年間の収量試験評価2020

    • Author(s)
      尹 棟敬、石山 敬貴、菅波 眞央、香川 昂亮、渡邊 まり、伊丸岡 芹菜、小倉 真紀、田副 雄士、石田 宏幸、鈴木 雄二、小原 実広、前 忠彦、牧野 周
    • Organizer
      日本植物生理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Rubisco量の抑制はイネの水ストレス耐性を低下させる2020

    • Author(s)
      鈴木 雄二、和田 慎也、三宅 親弘、牧野 周
    • Organizer
      日本植物生理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネにおけるRubiscoとRubisco activaseの同時過剰生産が光合成に与える影響2020

    • Author(s)
      菅波 眞央、 鈴木 雄二、 田副 雄士、 牧野 周
    • Organizer
      日本作物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 遺伝子組換え隔離ほ場におけるRubisco 過剰生産イネのバイオマス生産、窒素利用および収量解析2019

    • Author(s)
      尹 棟敬、石山敬貴、菅波眞央、香川昂亮、渡邊まり、伊丸岡芹菜、小倉真紀、田副雄士、石田宏幸、鈴木雄二、小原実広、前 忠彦、牧野 周
    • Organizer
      日本土壌肥料学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネ生葉における過剰なリン酸蓄積が枯死を引き起こすメカニズムの解明2019

    • Author(s)
      高木大輔、田副雄士、菅波眞央、上田晃弘、鈴木雄二、牧野 周
    • Organizer
      日本土壌肥料学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高温条件下で水ストレスを受けたイネにおける光合成電子伝達反応の応答と光化学系I 反応中心クロロフィルP700の酸化促進2019

    • Author(s)
      鈴木雄二、和田慎也、高木大輔、三宅親弘、牧野 周
    • Organizer
      日本土壌肥料学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネにおけるRubiscoの特異的増減が水ストレスへの感受性に及ぼす影響2019

    • Author(s)
      和田慎也、三宅親弘、牧野 周、鈴木雄二
    • Organizer
      日本土壌肥料学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Phosphorus toxicity decreases both electron sink activity and anti-oxidative activity in rice leaves2019

    • Author(s)
      Daisuke Takagi, Youshi Tazoe, Mao Suganami, Akihiro Ueda, Yuji Suzuki, Amane Makino
    • Organizer
      10th International Conference Photosynthesis and Hydrogen Energy Research for Sustainability
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Photorespiration contributes to oxidation of the reaction center chlorophyll of photosystem I and the robustness of photosystem I against excess light stress under CO2-limited conditions in rice.2019

    • Author(s)
      Wada S, Suzuki Y, Takagi D, Miyake C, Makino A
    • Organizer
      3rd Agriculture and Climate Change Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光呼吸による過剰光エネルギーに対する防御2019

    • Author(s)
      菅波眞央,鈴木雄二,田副雄士,牧野周
    • Organizer
      日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Rubisco activase過剰発現イネの光合成特性2019

    • Author(s)
      今野壮,菅波眞央,太田吉哉,高木大輔,田副雄士,鈴木雄二,牧野周
    • Organizer
      日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] リン酸毒性はRubiscoの活性化率と抗酸化活性の低下によってイネの生育を抑制する2019

    • Author(s)
      高木大輔,田副雄士,菅波眞央,上田晃弘,鈴木雄二,牧野周
    • Organizer
      日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Is there a trade-off between the amounts of Rubisco and Rubisco activase (RCA)?-Analysis of transgenic rice plants with changes in RCA or Rubisco contents-2018

    • Author(s)
      Suganami M, Suzuki Y, Nishida S, Konno S, Makino A
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Excess phosphorus declines the Rubisco activation state and inhibits photosynthesis in rice leaves2018

    • Author(s)
      Takagi D, Tazoe Y, Suganami M, Ueda A, Suzuki Y, Makino A
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rubisco過剰生産・生産抑制イネの隔離圃場栽培における光合成の解析2018

    • Author(s)
      菅波眞央,石山敬貴,渋谷暁一,渡邊まり,小倉真紀,尹棟敬,田副雄士,鈴木雄二,前忠彦,牧野周
    • Organizer
      日本光合成学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] イネにおけるP700吸光パラメーターによる水ストレス診断2018

    • Author(s)
      和田慎也,鈴木雄二,高木大輔,三宅親弘,牧野周
    • Organizer
      日本土壌肥料学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 光呼吸による過剰光エネルギーに対する防御2018

    • Author(s)
      鈴木雄二,和田慎也,高木大輔,三宅親弘,牧野周
    • Organizer
      日本土壌肥料学会
    • Related Report
      2018 Annual Research Report
    • Invited

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Published: 2018-04-23   Modified: 2022-01-27  

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