the mode of action in the combined-culture methods that induce potential secondary metabolism
Project/Area Number |
18H02120
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 38020:Applied microbiology-related
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Research Institution | The University of Tokyo |
Principal Investigator |
Hiroyasu Onaka 東京大学, 大学院農学生命科学研究科(農学部), 特任教授 (80315829)
|
Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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Keywords | 共培養 / 天然物スクリーニング / 抗生物質生産 / 放線菌 / ミコール酸 / 微生物間相互作用 / 二次代謝産物 / 複合培養 / 生合成 / 微生物相互作用 / 二次代謝 / ミコール酸含有細菌 |
Outline of Final Research Achievements |
Actinomycetes are known to conduct a wide variety of secondary metabolisms. Many secondary metabolites have been discovered from actinomycetes and have been used as pharmaceutical drugs. However, genome analysis has revealed that only a few secondary metabolites have been discovered in actinomycetes, and that many potential secondary metabolites have not yet been discovered. The discovery of such potential secondary metabolites is expected to lead to the development of new drugs. Therefore, we have established a "combined-culture" as a method to activate potential secondary metabolisms, and have already discovered over 35 new secondary metabolites. This method was inspired by the soil environment in which actinomycetes generally live, and it activates the potential secondary metabolism of actinomycetes by co-culture of mycolic acid-containing bacteria (MACB) and actinomycetes. In this study, we investigated the mode of action in the combined-culture.
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Academic Significance and Societal Importance of the Research Achievements |
天然物は医薬品の供給源として重要である。特に、昨今のコロナウイルスを初めとした多様な感染症被害は依然として人類の脅威であるため、新たな医薬品の開発は重要な研究テーマの一つである。複合培養法は微生物由来天然物の探索に有用な技術である。多様な天然物を生産することが知られている放線菌とミコール酸含有細菌を共培養することにより、従来の純粋培養では生産しなかった様々な天然物を放線菌が生産するようになる。今回の研究では、複合培養の作用機構解明を進めた。また、複合培養を用いた天然物探索も進め、新たに12種類の新規天然物の発見に成功した。
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Longicatenamides A-D, two diastereomeric pairs of cyclic hexapeptides produced by combined-culture of Streptomyces sp. KUSC_F05 and Tsukamurella pulmonis TP-B05962021
Author(s)
Jiang, Y., Matsumoto, T., Kuranaga, T., Lu, S., Wang, W., Onaka, H., Kakeya, H.
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Journal Title
J. Antibiot.
Volume: -
Issue: 5
Pages: 307-316
DOI
Related Report
Peer Reviewed
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[Journal Article] Amycolapeptins A and B, cyclic nonadepsipeptides produced by combined-culture of Amicolatopsis sp. and Tsukamurella pulmonis2021
Author(s)
Pan, C., Kuranaga, T., Cao, X., Suzuki, T., Dohmae, N., Shinzato, N., Onaka, H., Kakeya, H.
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Journal Title
J. Org. Chem.
Volume: 86
Issue: 2
Pages: 1843-1849
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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