Molecular mechanisms of energy metabolic state transition in bacteria
| Project/Area Number |
18H02124
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Tanaka Kan 東京工業大学, 科学技術創成研究院, 教授 (60222113)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
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| Keywords | エネルギー代謝ステート / 大腸菌 / シアノバクテリア / 中央代謝経路 / 明暗環境応答 / 光合成 / 酢酸オーバーフロー代謝 |
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| Outline of Final Research Achievements |
Bacteria significantly change the mode of energy acquisition and metabolism due to changes in the growing environment. In this study, we used Escherichia coli and cyanobacteria as research materials to analyze the molecular mechanism by which bacteria significantly change their metabolic state. Escherichia coli changes its metabolic state significantly depending on the presence or absence of glucose in the medium, but conventionally this state transition has been explained by the change in gene expression state. In this study, we elucidated the molecular mechanism involved in the activation of specific enzymes by acetate overflow metabolism resulting in the induction of large-scale changes in metabolic flux. In addition, we approached the adaptive mechanism of cyanobacteria associated with the shift of light and dark states from the viewpoint of transcriptional regulation mechanism.
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| Academic Significance and Societal Importance of the Research Achievements |
細胞は様々な代謝様式で必要なエネルギーを獲得する能力をもち、それらを置かれた環境により切り替えて生存している。そして、これらの代謝ステートそれぞれの理解が進んでいる現在でも、一つの状態から別の状態に遷移する動的な分子機構の解明は進んでいない。本研究では、グルコースで培養した大腸菌がグルコースから他の炭素減にエネルギー源を切り替える遷移現象、また、シアノバクテリアが光環境に合わせて生理状態を切り替える現象の解析を合わせて行った。従来のような時間を止めたスナップショットの研究だけでなく、動的遷移の制御に注目することで、大腸菌では酢酸オーバーフロー代謝の全く新しい生理的意義を発見できた。
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] ESCRT machinery mediates cytokinetic abscission in the unicellular red alga Cyanidioschyzon merolae.2020
Author(s)
Yagisawa F., Fujiwara T., Takemura T., Kobayashi Y., Sumiya N., Miyagishima S. Y., Nakamura S., Imoto Y., Misumi O., Tanaka K., Kuroiwa H., Kuroiwa T.
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Journal Title
Frontiers in Cell and Developmental Biology
Volume: 8
Pages: 169-169
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] The retrograde signaling protein GUN1 regulates tetrapyrrole biosynthesis.2019
Author(s)
Shimizu, T., Kacprzak, S.M., Mochizuki, N., Nagatani, A., Watanabe, S., Shimada, T., Tanaka, K., Hayashi, Y., Arai, M., Leister, D., Okamoto, H., Terry, M.J., & Masuda, T.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 116
Issue: 49
Pages: 24900-24906
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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