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Molecular mechanisms of energy metabolic state transition in bacteria

Research Project

Project/Area Number 18H02124
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionTokyo Institute of Technology

Principal Investigator

Tanaka Kan  東京工業大学, 科学技術創成研究院, 教授 (60222113)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Keywordsエネルギー代謝ステート / 大腸菌 / シアノバクテリア / 中央代謝経路 / 明暗環境応答 / 光合成 / 酢酸オーバーフロー代謝
Outline of Final Research Achievements

Bacteria significantly change the mode of energy acquisition and metabolism due to changes in the growing environment. In this study, we used Escherichia coli and cyanobacteria as research materials to analyze the molecular mechanism by which bacteria significantly change their metabolic state. Escherichia coli changes its metabolic state significantly depending on the presence or absence of glucose in the medium, but conventionally this state transition has been explained by the change in gene expression state. In this study, we elucidated the molecular mechanism involved in the activation of specific enzymes by acetate overflow metabolism resulting in the induction of large-scale changes in metabolic flux. In addition, we approached the adaptive mechanism of cyanobacteria associated with the shift of light and dark states from the viewpoint of transcriptional regulation mechanism.

Academic Significance and Societal Importance of the Research Achievements

細胞は様々な代謝様式で必要なエネルギーを獲得する能力をもち、それらを置かれた環境により切り替えて生存している。そして、これらの代謝ステートそれぞれの理解が進んでいる現在でも、一つの状態から別の状態に遷移する動的な分子機構の解明は進んでいない。本研究では、グルコースで培養した大腸菌がグルコースから他の炭素減にエネルギー源を切り替える遷移現象、また、シアノバクテリアが光環境に合わせて生理状態を切り替える現象の解析を合わせて行った。従来のような時間を止めたスナップショットの研究だけでなく、動的遷移の制御に注目することで、大腸菌では酢酸オーバーフロー代謝の全く新しい生理的意義を発見できた。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (44 results)

All 2021 2020 2019 2018

All Journal Article (18 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 18 results,  Open Access: 10 results) Presentation (26 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results)

  • [Journal Article] The Circadian Rhythm Regulator RpaA Modulates Photosynthetic Electron Transport and Alters the Preferable Temperature Range for Growth in a Cyanobacterium2021

    • Author(s)
      Hazuki Hasegawa, Tatsuhiro Tsurumaki, Sousuke Imamura, Kintake Sonoike and Kan Tanaka
    • Journal Title

      FEBS Letters

      Volume: published online Issue: 10 Pages: 1480-1492

    • DOI

      10.1002/1873-3468.14075

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of a firefly luciferase reporter assay system in the unicellular red alga <i>Cyanidioschyzon merolae</i>2021

    • Author(s)
      Baifeng Zhou, Sota Takahashi, Tokiaki Takemura, Kan Tanaka and Sousuke Imamura
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 67 Issue: 1 Pages: 42-46

    • DOI

      10.2323/jgam.2020.02.003

    • NAID

      130008025418

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Proteomic analysis of haem-binding protein from Arabidopsis thaliana and Cyanidioschyzon merolae2020

    • Author(s)
      Takayuki Shimizu, Rintaro Yasuda, Yui Mukai, Ryo Tanoue, Tomohiro Shimada, Sousuke Imamura, Kan Tanaka, Satoru Watanabe and Tatsuru Masuda
    • Journal Title

      Philosophical Transactions of the Royal Society B

      Volume: 375 Issue: 1801 Pages: 20190488-20190488

    • DOI

      10.1098/rstb.2019.0488

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ESCRT machinery mediates cytokinetic abscission in the unicellular red alga Cyanidioschyzon merolae.2020

    • Author(s)
      Yagisawa F., Fujiwara T., Takemura T., Kobayashi Y., Sumiya N., Miyagishima S. Y., Nakamura S., Imoto Y., Misumi O., Tanaka K., Kuroiwa H., Kuroiwa T.
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 8 Pages: 169-169

    • DOI

      10.3389/fcell.2020.00169

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Measurement of the redox state of the plastoquinone pool in cyanobacteria2020

    • Author(s)
      Sergey Khorobrykh, Tatsuhiro Tsurumaki, Kan Tanaka, Taina Tyystjarvi and Esa Tyystjarvi
    • Journal Title

      FEBS Lett.

      Volume: 594 Issue: 2 Pages: 367-375

    • DOI

      10.1002/1873-3468.13605

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification and analysis of a principal sigma factor interacting protein SinA, essential for growth at high temperatures in a cyanobacterium <i>Synechococcus elongatus</i> PCC 79422020

    • Author(s)
      Hazuki Hasegawa, Tatsuhiro Tsurumaki, Ikki Kobayashi, Sousuke Imamura and Kan Tanaka
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 66 Issue: 2 Pages: 66-72

    • DOI

      10.2323/jgam.2019.05.002

    • NAID

      130007855492

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A suicide enzyme catalyzes multiple reactions for biotin biosynthesis in cyanobacteria2020

    • Author(s)
      Kei Sakaki, Keita Ohishi, Tetsu Shimizu, Ikki Kobayashi, Naoki Mori, Kenichi Matsuda, Takeo Tomita, Hidenori Watanabe, Kan Tanaka, Tomohisa Kuzuyama and Makoto Nishiyama
    • Journal Title

      Nature Chem. Biol.

      Volume: 16 Issue: 4 Pages: 415-422

    • DOI

      10.1038/s41589-019-0461-9

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Top Starch Plating Method for the Efficient Cultivation of Unicellular Red Alga Cyanidioschyzon merolae2019

    • Author(s)
      Takemura Tokiaki、Kobayashi Yuki、Imamura Sousuke、Tanaka Kan
    • Journal Title

      BIO-PROTOCOL

      Volume: 9 Issue: 4

    • DOI

      10.21769/bioprotoc.3172

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Overexpression of a glycogenin, CmGLG2, enhances floridean starch accumulation in the red alga Cyanidioschyzon merolae2019

    • Author(s)
      Imran Pancha, Kan Tanaka and Sousuke Imamura
    • Journal Title

      Plant Signal. Behav.

      Volume: 14 Issue: 6 Pages: 1596718-1596718

    • DOI

      10.1080/15592324.2019.1596718

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple modification of chromosomal loci using URA5.3 selection marker in the unicellular red alga Cyanidioschyzon merolae.2019

    • Author(s)
      Tokiaki Takemura, Sousuke Imamura, Yuki Kobayashi and Kan Tanaka
    • Journal Title

      Bio-protocol

      Volume: 9 Issue: 7

    • DOI

      10.21769/bioprotoc.3204

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The retrograde signaling protein GUN1 regulates tetrapyrrole biosynthesis.2019

    • Author(s)
      Shimizu, T., Kacprzak, S.M., Mochizuki, N., Nagatani, A., Watanabe, S., Shimada, T., Tanaka, K., Hayashi, Y., Arai, M., Leister, D., Okamoto, H., Terry, M.J., & Masuda, T.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 116 Issue: 49 Pages: 24900-24906

    • DOI

      10.1073/pnas.1911251116

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of a chloroplast fatty acid exporter protein, CmFAX1, and triacylglycerol accumulation by its overexpression in the unicellular red alga Cyanidioschyzon merolae2019

    • Author(s)
      Tokiaki Takemura, Sousuke Imamura and Kan Tanaka
    • Journal Title

      Algal Research

      Volume: 38 Pages: 101396-101396

    • DOI

      10.1016/j.algal.2018.101396

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] C-terminal regulatory domain of the ε subunit of FoF1 ATP synthase enhances the ATP-dependent H+ pumping that is involved in the maintenance of cellular membrane potential in Bacillus subtilis2019

    • Author(s)
      Akanuma Genki、Tagana Tomoaki、Sawada Maho、Suzuki Shota、Shimada Tomohiro、Tanaka Kan、Kawamura Fujio、Kato-Yamada Yasuyuki
    • Journal Title

      MicrobiologyOpen

      Volume: - Issue: 8

    • DOI

      10.1002/mbo3.815

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lability in sulfur acidic cultivation medium explains unstable effects of CDK inhibitors on <i>Cyanidioschyzon merolae</i> cell proliferation2018

    • Author(s)
      Yuki Kobayashi and Kan Tanaka
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 64 Issue: 6 Pages: 299-302

    • DOI

      10.2323/jgam.2018.01.002

    • NAID

      130007558395

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The checkpoint kinase TOR (target of rapamycin) regulates expression of a nuclear-encoded chloroplast RelA-SpoT homolog (RSH) and modulates chloroplast ribosomal RNA synthesis in a unicellular red alga2018

    • Author(s)
      Imamura Sousuke、Nomura Yuhta、Takemura Tokiaki、Pancha Imran、Taki Keiko、Toguchi Kazuki、Tozawa Yuzuru、Tanaka Kan
    • Journal Title

      Plant J

      Volume: 94 Issue: 2 Pages: 327-339

    • DOI

      10.1111/tpj.13859

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of a Selectable Marker Recycling System and the Use in Epitope Tagging of Multiple Nuclear Genes in the Unicellular Red Alga Cyanidioschyzon merolae2018

    • Author(s)
      Takemura Tokiaki、Imamura Sousuke、Kobayashi Yuki、Tanaka Kan
    • Journal Title

      Plant and Cell Physiology

      Volume: 59 Pages: 2308-2316

    • DOI

      10.1093/pcp/pcy156

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Accelerated triacylglycerol production without growth inhibition by overexpression of a glycerol-3-phosphate acyltransferase in the unicellular red alga Cyanidioschyzon merolae2018

    • Author(s)
      Fukuda Satoshi、Hirasawa Eri、Takemura Tokiaki、Takahashi Sota、Chokshi Kaumeel、Pancha Imran、Tanaka Kan、Imamura Sousuke
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 12410-12410

    • DOI

      10.1038/s41598-018-30809-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Target of rapamycin-signaling modulates starch accumulation via glycogenin phosphorylation status in the unicellular red alga Cyanidioschyzon merolae2018

    • Author(s)
      Pancha Imran、Shima Hiroki、Higashitani Nahoko、Igarashi Kazuhiko、Higashitani Atsushi、Tanaka Kan、Imamura Sousuke
    • Journal Title

      The Plant Journal

      Volume: 97 Issue: 3 Pages: 485-499

    • DOI

      10.1111/tpj.14136

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] 大腸菌における代謝ステート遷移機構の解析:2-オキソグルタル酸デヒドロゲナーゼ活性化における酢酸とリポ酸化の役割2021

    • Author(s)
      中澤 昂太 、田中 寛
    • Organizer
      日本農芸化学会 2021年度(令和3年度)大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シアノバクテリアにおいて時計制御因子RpaAが光合成電子伝達制御を介して増殖至適温度を制御する2021

    • Author(s)
      長谷川葉月、鶴巻達大、今村壮輔、園池公毅、田中寛
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シアノバクテリアと紅藻葉緑体に保存された二成分制御系による多様な光強度変化に際した転写制御2021

    • Author(s)
      安田 暉、伊南 大地、今村 壮輔、田中 寛、華岡 光正
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シアノバクテリアヒスチジンキナーゼNblSは進化の過程で消失したPSII結合型センサーである2021

    • Author(s)
      鶴巻 達大、田中 寛
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 原始紅藻における光シグナル伝達に関わるE3ユビキチンリガーゼCul4複合体の解析2021

    • Author(s)
      小林 勇気、北川 美也子、吉川 瞳子、大原 ひかる、華岡 光正、今村 壮輔、田中 寛
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 単細胞紅藻シゾンにおける窒素欠乏応答転写因子 MYB1 の活性化調節機構2021

    • Author(s)
      Zhou Baifeng、島 弘季、五十嵐 和彦、田中 寛、今村 壮輔
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 単細胞紅藻 Cyanidioschyzon merolaeにおいて ACRドメインリピートタンパク質 CmACR は窒素同化を 担うGS/GOGAT サイクルを統合する2020

    • Author(s)
      竹村時空,今村壮輔,田中寛
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Characterization of Target of Rapamycin (TOR) Complex in the Unicellular Red Alga.2020

    • Author(s)
      Kaumeel Choksi, Kan Tanaka, Sousuke Imamura
    • Organizer
      The 61th JSPP annual meeting
    • Related Report
      2019 Annual Research Report
  • [Presentation] 単細胞紅藻シゾンにおけるスターチ蓄積を調節する新規機構の解明2020

    • Author(s)
      Imran Pancha,島弘季,東谷なほ子,五十嵐和彦,東谷篤志,田中寛,今村壮輔
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 単細胞紅藻シゾンにおける窒素欠乏応答転写因子 MYB1 の活性化調節機構2020

    • Author(s)
      周柏峰,田中寛,今村壮輔
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] E3ユビキチンリガーゼ Cul4 複合体の関わる単細胞紅藻光シグナル伝達経路2020

    • Author(s)
      北川美也子,小林勇気,吉川瞳子,大原ひかる,華岡光正,今村壮輔,田中寛
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Analysis of initiation of organelle DNA replication in red alga Cyanidioschyzon merolae2020

    • Author(s)
      小林勇気、佐藤 伸一、丹羽 達也、田口英樹、中村浩之、田中寛
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ルシフェラーゼ発光による枯草菌細胞内 NADPH レベルの非破壊的観測2020

    • Author(s)
      吉田 健一 、石川 周 、田中 寛
    • Organizer
      日本農芸化学会 2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 大腸菌代謝ステート遷移における酢酸の役割2020

    • Author(s)
      中澤 昂太 、太刀川 智之 、島田 友裕 、田中 寛
    • Organizer
      日本農芸化学会 2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 大腸菌エネルギー代謝遷移におけるPDH量のコントロールとPdhRの役割2019

    • Author(s)
      太刀川智之、島田友裕、丹羽達也、田口英樹、田中寛
    • Organizer
      第13回 日本ゲノム微生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シアノバクテリアにおける主要シグマ因子結合タンパク質と熱耐性2019

    • Author(s)
      長谷川葉月、鶴巻達大、小林一幾、今村壮輔、田中寛
    • Organizer
      第13回 日本ゲノム微生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolaeにおけるグルタミン結合ドメインリピートタンパク質CmACRの機能解析2019

    • Author(s)
      竹村時空、今村壮輔、田中寛
    • Organizer
      第60回日本植物生理学会年会・ポスター
    • Related Report
      2018 Annual Research Report
  • [Presentation] シアノバクテリアSynechococcus elongatus PCC 7942における主要シグマ因子結合タンパク質の機能解析2019

    • Author(s)
      長谷川葉月、鶴巻達大、小林一幾、今村壮輔、田中寛
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolaeにおける葉緑体局在脂肪酸輸送体CmFAX1の同定とその応用2019

    • Author(s)
      竹村時空、今村壮輔、田中寛
    • Organizer
      日本農芸化学会2019年度東京大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 大腸菌の炭素源シフトにおけるオーバーフロー代謝の役割2019

    • Author(s)
      田中寛、太刀川智之、丹羽達也、田口英樹、齊藤菜摘、島田友裕
    • Organizer
      日本農芸化学会2019年度東京大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Information processing from light to gene expression in cyanobacteria2018

    • Author(s)
      Kan Tanaka
    • Organizer
      Japan-Finland Seminar 2018, Shaping photosynthesis against climate change and toward efficient water and nutrient management
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Analysis of photosynthesis and photoinhibition gated by intracellular circadian rhythm in a cyanobacterium Synechococcus elongatus PCC 79422018

    • Author(s)
      Tatsuhiro Tsurumaki and Kan Tanaka
    • Organizer
      Japan-Finland Seminar 2018, Shaping photosynthesis against climate change and toward efficient water and nutrient management
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Chloroplast transcription and the regulation in C. merolae2018

    • Author(s)
      Kan Tanaka
    • Organizer
      International Symposium "Cyanidioschyzon merolae as an arising model for unicellular eukaryotes":
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Abscisic Acid Signaling in a Unicellular Red Alga2018

    • Author(s)
      Kan Tanaka
    • Organizer
      6th Plant Dormancy Symposium 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] シアノバクテリアの高振幅シグマ因子群による概日時計に依存した転写制御2018

    • Author(s)
      伊南大地、徳山城永、小堀奈美、秋元勇輝、田中寛、華岡光正
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 大腸菌の炭素源シフトにおけるオーバフロー代謝の役割2018

    • Author(s)
      田中 寛
    • Organizer
      第41回日本分子生物学会年会・ワークショップ「微生物の増殖とふるまいの複雑性:多様なモデル微生物系から到達する新たな理解」
    • Related Report
      2018 Annual Research Report
    • Invited

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Published: 2018-04-23   Modified: 2022-01-27  

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