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Diversity of acyl groups of phospholipids in bacterial cell membranes: Its generation mechanism and physiological significance

Research Project

Project/Area Number 18H02127
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionKyoto University

Principal Investigator

KURIHARA Tatsuo  京都大学, 化学研究所, 教授 (70243087)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords生体膜 / リン脂質 / リゾホスファチジン酸アシル基転移酵素 / 高度不飽和脂肪酸 / PlsC / 分岐鎖脂肪酸
Outline of Final Research Achievements

We analyzed the function of lysophosphatidic acid acyltransferase (LPAAT), which is responsible for the generation of sn-2 acyl chain diversity in bacterial membrane phospholipids. We identified a novel LPAAT, YihG, in Escherichia coli, and clarified its substrate specificity and its relationship to flagellar formation. We succeeded in purification of LPAAT of Thermus thermophilus HB8 and clarified its various properties. Mutant strains of Shewanella livingstonensis Ac10 with inactivated LPAAT paralogs, PlsC4 and PlsC5, were generated, and the substrate specificities of these LPAATs and the involvement of PlsC4 in low temperature adaptation were demonstrated.

Academic Significance and Societal Importance of the Research Achievements

生体膜を構成するリン脂質には大きな構造多様性がある。構造が異なる種々のリン脂質を適切に作り分けることは、生体膜の機能発現に必須である。本研究はリン脂質のアシル鎖多様性を生み出す酵素群の機能を明らかにしたものであり、生体膜構築機構の解明に向けた基盤的知見をあたえるものである。また、膜のリン脂質組成は物質の膜透過性に影響を及ぼすことから、微生物細胞を利用した発酵生産など、物質の膜透過性が生産効率に影響するプロセスを開発する上で、膜リン脂質組成の制御機構に関する理解は重要であり、そのような観点から、本研究成果は応用微生物学的にも意義深い。

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (22 results)

All 2021 2020 2019

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 4 results) Presentation (15 results) (of which Invited: 1 results)

  • [Journal Article] Identification of a Putative Sensor Protein Involved in Regulation of Vesicle Production by a Hypervesiculating Bacterium, Shewanella vesiculosa HM132021

    • Author(s)
      Fumiaki Yokoyama、Tomoya Imai、Wataru Aoki、Mitsuyoshi Ueda、Jun Kawamoto、Tatsuo Kurihara
    • Journal Title

      Frontiers in Microbiology

      Volume: 12 Pages: 629023-629023

    • DOI

      10.3389/fmicb.2021.629023

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of acyl-CoA dehydrogenases from Shewanella livingstonensis Ac10 in docosahexaenoic acid conversion2020

    • Author(s)
      Yustina Yusuf, Takuya Ogawa, Jun Kawamoto, Tatsuo Kurihara
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 528 Issue: 3 Pages: 453-458

    • DOI

      10.1016/j.bbrc.2020.05.185

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Lysophosphatidic Acid Acyltransferase of Escherichia coli Produces Membrane Phospholipids with a cis-vaccenoyl Group and Is Related to Flagellar Formation.2020

    • Author(s)
      Toyotake Y, Nishiyama M, Yokoyama F, Ogawa T, Kawamoto J, Kurihara T.
    • Journal Title

      Biomolecules

      Volume: 11 Issue: 5 Pages: 745-745

    • DOI

      10.3390/biom10050745

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bioconversion From Docosahexaenoic Acid to Eicosapentaenoic Acid in the Marine Bacterium Shewanella livingstonensis Ac102020

    • Author(s)
      Ogawa Takuya、Hirose Kazuki、Yusuf Yustina、Kawamoto Jun、Kurihara Tatsuo
    • Journal Title

      Frontiers in Microbiology

      Volume: 11 Pages: 1-12

    • DOI

      10.3389/fmicb.2020.01104

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lysophosphatidic acid acyltransferase from the thermophilic bacterium <i>Thermus thermophilus</i> HB8 displays substrate promiscuity2020

    • Author(s)
      Ogawa Takuya、Suwanawat Nittikarn、Toyotake Yosuke、Watanabe Bunta、Kawamoto Jun、Kurihara Tatsuo
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 84 Issue: 9 Pages: 1831-1838

    • DOI

      10.1080/09168451.2020.1771169

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of a novel bacterial strain capable of producing abundant extracellular membrane vesicles carrying a single major cargo protein and analysis of its transport mechanism2020

    • Author(s)
      Chen Chen、Jun Kawamoto、Soichiro Kawai、Akihiro Tame、Chiaki Kato、Tomoya Imai、Tatsuo Kurihara
    • Journal Title

      Frontiers in Microbiology

      Volume: 10 Pages: 3001-3001

    • DOI

      10.3389/fmicb.2019.03001

    • NAID

      120006796472

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic characterization and functional implications of the gene cluster for selective protein transport to extracellular membrane vesicles of Shewanella vesiculosa HM132020

    • Author(s)
      Kouhei Kamasaka、Jun Kawamoto、Chen Chen、Fumiaki Yokoyama、Tomoya Imai、Takuya Ogawa、Tatsuo Kurihara
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: - Issue: 2 Pages: 525-531

    • DOI

      10.1016/j.bbrc.2020.03.125

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] In vitro characterization of lysophosphatidic acid acyltransferase (PlsC) from Escherichia coli2021

    • Author(s)
      Nittikarn Suwanawat、Takuya Ogawa、Jun Kawamoto、Tatsuo Kurihara
    • Organizer
      第94回日本生化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] DHAからEPAへの代謝変換に関与するアシルCoAデヒドロゲナーゼ2020

    • Author(s)
      栗原達夫
    • Organizer
      ビタミンB研究委員会 第460回研究協議会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 海洋性細菌によるω-3高度不飽和脂肪酸の新奇代謝変換能2020

    • Author(s)
      小川拓哉、廣瀬和樹、Yustina Yusuf、川本純、栗原達夫
    • Organizer
      日本ビタミン学会第72回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Comparative analysis of lysophosphatidic acid acyltransferase homologs2020

    • Author(s)
      Misaki Kuboshima、Nittikarn Suwanawat、Jun Kawamoto、Takuya Ogawa、Tatsuo Kurihara
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 におけるω-3系高度不飽和脂肪酸の代謝様式の解析2020

    • Author(s)
      小川拓哉、Yustina Yusuf、川本純、栗原達夫
    • Organizer
      第21回極限環境生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 低温菌 Shewanella vesiculosa HM13 における膜小胞生産への膜リン脂質の関与2020

    • Author(s)
      中野咲梨、小川拓哉、川本純、栗原達夫
    • Organizer
      第21回極限環境生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] In vitro and In vivo analysis of FadH protein from Shewanella livingstonensis Ac10 involved in DHA conversion2020

    • Author(s)
      Yustina Yusuf、廣瀬和樹、川本純、小川拓哉、栗原達夫
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] リゾホスファチジン酸アシル基転移酵素ホモログの比較解析2020

    • Author(s)
      久保嶋美咲、Nittikarn Suwanawat、川本純、小川拓哉、栗原達夫
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 海洋性 Shewanella 属細菌による ω-3 系高度不飽和脂肪酸の変換経路の解明2019

    • Author(s)
      小川拓哉、廣瀬和樹、川本純、栗原達夫
    • Organizer
      第66回日本生化学会近畿支部例会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細菌における長鎖多価不飽和脂肪酸結合型タンパク質の探索2019

    • Author(s)
      川本純、米田雄紀、小川拓哉、栗原達夫
    • Organizer
      第61回日本脂質生化学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Shewanella 属細菌における新規エイコサペンタエン酸生産経路の解析2019

    • Author(s)
      小川拓哉、廣瀬和樹、川本純、栗原達夫
    • Organizer
      酵素・補酵素研究会2019
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細菌生体膜機能発現の分子基盤: 膜リン脂質・膜小胞研究の新展開2019

    • Author(s)
      栗原達夫
    • Organizer
      第33回ケミカルバイオロジー研究所セミナー/第105回生物科学フロンティアセミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Polyunsaturated fatty acid conversion in Shewanella livingstonensis Ac102019

    • Author(s)
      Yusina Yusuf、廣瀬和樹、小川拓哉、川本純、栗原達夫
    • Organizer
      第92回日本生化学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Shewanella livingstonensis Ac10 の多価不飽和脂肪酸の変換における FadE の役割2019

    • Author(s)
      Yustina Yusuf、廣瀬和樹、小川拓哉、川本純、栗原達夫
    • Organizer
      第20回極限環境生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 低温菌 Shewanella livingstonensis Ac10 におけるリゾホスファチジン酸アシル基転移酵素 PlsC5 の機能解析2019

    • Author(s)
      久保嶋美咲、小川拓哉、川本純、栗原達夫
    • Organizer
      第20回極限環境生物学会年会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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