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Importance of lanthanides in microorganisms

Research Project

Project/Area Number 18H02129
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionOkayama University

Principal Investigator

Tani Akio  岡山大学, 資源植物科学研究所, 准教授 (00335621)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
KeywordsMethylotroph / Lanthanides / Methanol dehydrogenase / Methylotrophs / Methylobacterium / lanthanide / methylotrophs / lanthanides / xoxF
Outline of Final Research Achievements

Methylobacterium species are phytosymbionts utilizing methanol emitted from plants. They utilize two different methanol dehydrogenases (MDHs) that require calcium and lanthanides. We examined the regulation mechanism of Ln-dependent gene expression, and found factors involved in the regulation. On the other hand, Ln-dependent MDH gene is encoded in many bacterial genomes. We found and characterized new Ln-dependent methylotrophs.

Academic Significance and Societal Importance of the Research Achievements

ランタノイドは天然には広く存在するが実験室環境には存在しないため、それに依存する微生物の生育、代謝系の制御の理解は自然界での微生物の振る舞いを理解する上で重要である。本研究ではランタノイドを生育に要求する微生物を新規に見いだし、その依存性をゲノムや酵素遺伝子の観点から証明した。またMethylobacterium属細菌についてランタノイドによる代謝酵素遺伝子制御のメカニズムの一端を明らかに出来た。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (41 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (6 results) Journal Article (12 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 12 results,  Open Access: 8 results) Presentation (23 results) (of which Invited: 1 results)

  • [Int'l Joint Research] ムグラ大学(トルコ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Skryabin Institute(ロシア連邦)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Mugla Sitka Kocman University(トルコ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Swinburne University(マレーシア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Burapha University(タイ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Jomo Kenyatta University(ケニア)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Discovery of lanthanide-dependent methylotrophy and screening methods for lanthanide-dependent methylotrophs2021

    • Author(s)
      Tani Akio、Mitsui Ryoji、Nakagawa Tomoyuki
    • Journal Title

      Methods in Enzymology

      Volume: 650 Pages: 1-18

    • DOI

      10.1016/bs.mie.2021.01.031

    • ISBN
      9780128238561
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preference for particular lanthanide species and thermal stability of XoxFs in Methylorubrum extorquens strain AM1.2020

    • Author(s)
      Wang L., Hibino A., Suganuma S., Ebihara A., Iwanoto S., Mitsiu R., Tani A. ,Shimada M., Hayakawa T., Nakagawa T.
    • Journal Title

      Enzyme and Microbial Technology

      Volume: 136 Pages: 109518-109518

    • DOI

      10.1016/j.enzmictec.2020.109518

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methylotenera oryzisoli sp. nov., a lanthanide-dependent methylotrophic bacteria isolated from rice field soil2020

    • Author(s)
      Lv Haoxin、Sahin Nurettin、Tani Akio
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 70 Issue: 4 Pages: 2713-2718

    • DOI

      10.1099/ijsem.0.004098

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lanthanide-Dependent Methanol and Formaldehyde Oxidation in Methylobacterium aquaticum Strain 22A2020

    • Author(s)
      Patcha Yanpirat, Yukari Nakatsuji, Shota Hiraga, Yoshiko Fujitani, Terumi Izumi, Sachiko Masuda, Ryoji Mitsui, Tomoyuki Nakagawa and Akio Tani
    • Journal Title

      microorganisms

      Volume: 8 Issue: 6 Pages: 822-822

    • DOI

      10.3390/microorganisms8060822

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulation of lanthanide-dependent methanol oxidation pathway in the legume symbiotic nitrogen-fixing bacterium Bradyrhizobium sp. strain Ce-32020

    • Author(s)
      Viagian Pastawan, Soya Suganuma, Kosuke Mizuno, Lun Wang, Akio Tani, Ryoji Mitsui, Kohei Nakamura, Masaya Shimada, Takashi Hayakawa, Nanung Agus Fitriyanto, and Tomoyuki Nakagawa
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 130 Issue: 6 Pages: 582-587

    • DOI

      10.1016/j.jbiosc.2020.07.012

    • NAID

      40022443010

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lanthanide-dependent methanol dehydrogenase from the legume symbiotic nitrogen-fixing bacterium Bradyrhizobium diazoefficiens strain USDA1102019

    • Author(s)
      Wang Lun、Suganuma Soya、Hibino Ayumi、Mitsui Ryoji、Tani Akio、Matsumoto Takashi、Ebihara Akio、Fitriyanto Nanung Agus、Pertiwiningrum Ambar、Shimada Masaya、Hayakawa Takashi、Nakagawa Tomoyuki
    • Journal Title

      Enzyme and Microbial Technology

      Volume: 130 Pages: 109371-109371

    • DOI

      10.1016/j.enzmictec.2019.109371

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bacteria with natural chemotaxis towards methanol revealed by chemotaxis fishing technique.2019

    • Author(s)
      Hamba Tola Y, Fujitani Y, Tani A.
    • Journal Title

      Biosci Biotech Biochem

      Volume: 83 Issue: 11 Pages: 2163-2171

    • DOI

      10.1080/09168451.2019.1637715

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Lanthanide-Dependent Regulation of Methylotrophy in Methylobacterium aquaticum Strain 22A2018

    • Author(s)
      Masuda Sachiko、Suzuki Yutaka、Fujitani Yoshiko、Mitsui Ryoji、Nakagawa Tomoyuki、Shintani Masaki、Tani Akio
    • Journal Title

      mSphere

      Volume: 3 Issue: 1

    • DOI

      10.1128/msphere.00462-17

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Isolation and genomic characterization of Novimethylophilus kurashikiensis gen. nov. sp. nov., a new lanthanide-dependent methylotrophic species of Methylophilaceae.2018

    • Author(s)
      Lv H, Sahin N, Tani A
    • Journal Title

      Environ Microbiol

      Volume: 20 Issue: 3 Pages: 1204-1223

    • DOI

      10.1111/1462-2920.14062

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Distribution of 1-aminocyclopropane-1-carboxylate deaminase and D-cysteine desulfhydrase genes among type species of the genus Methylobacterium.2018

    • Author(s)
      Ekimova GA, Fedorov DN, Tani A, Doronina NV, Trotsenko YA.
    • Journal Title

      Antonie Van Leeuwenhoek

      Volume: - Issue: 10 Pages: 1-12

    • DOI

      10.1007/s10482-018-1061-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ergothioneine production using Methylobacterium species, yeast, and fungi.2018

    • Author(s)
      Fujitani Y., Alamgir M.K. Tani A.
    • Journal Title

      J Biosci Bioeng

      Volume: 126 Issue: 6 Pages: 715-722

    • DOI

      10.1016/j.jbiosc.2018.05.021

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genomic characterization of methylotrophy of Oharaeibacter diazotrophicus strain SM30T.2018

    • Author(s)
      Lv H, Tani A
    • Journal Title

      J Biosci Bioeng

      Volume: 126 Issue: 6 Pages: 667-675

    • DOI

      10.1016/j.jbiosc.2018.05.023

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] Bacteria with natural chemotaxis towards methanol revealed by chemotaxis fishing technique2021

    • Author(s)
      谷 明生
    • Organizer
      日本農芸化学会 中四国支部 第58回講演会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Molecular mechanism of methylotaxis in Methylobacterium aquaticum strain 22A2021

    • Author(s)
      菊池志保、藤谷良子、加藤純一、谷 明生
    • Organizer
      日本農芸化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Comparative genomic analysis of the pink pigmented facultative methylotrophs (PPFM)2021

    • Author(s)
      Ola Alessa, Nurettin Sahin, Yoshihisa Ogura, Tetsuya Hayashi, Akio Tani
    • Organizer
      日本農芸化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Genetic and functional analysis of siderophore in Methylobacterium aquaticum 22A2021

    • Author(s)
      Patrick Juma, Ola Alessa, Yoshiko Fujitani, Tokitaka Oyama, Hiroya Yurimoto, Yasuyoshi Sakai, Akio Tani
    • Organizer
      日本農芸化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 蛍光タンパク質を発現させた Methylobacterium 属細菌のアカシソ上での動態解析2021

    • Author(s)
      片山志織、由里本博也、谷明生、阪井康能
    • Organizer
      日本農芸化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物葉上共生細菌Methylorubrum extorquens AM1のランタノイド依存型メタノールデヒドロゲナーゼアイソザイムのレポーター遺伝子を用いた発現解析2020

    • Author(s)
      山下颯太,吉川友理, 谷 明生,中川智行,矢野嵩典,三井亮司
    • Organizer
      農芸化学会中四国支部講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物葉上共生細菌Methylorubrum extorquens AM1のランタノイド依存型メタノールデヒドロゲナーゼアイソザイムXoxF2の解析2020

    • Author(s)
      竹内赴登、山下颯太、谷 明生、中川智行、矢野嵩典、三井亮司
    • Organizer
      農芸化学会中四国支部講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Methylobacterium 属細菌におけるメタノール走化性の分子メカニズムの解明2020

    • Author(s)
      菊池志保、谷 明生
    • Organizer
      岡山バイオアクティブ研究会第57回シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] Methylorubrum属細菌の低栄養環境下におけるランタノイド応答の分子メカニズムの解明2020

    • Author(s)
      水野洸介,原田雄斗,岩本悟志,谷明生,三井亮司,島田昌也,早川享志,中川智行
    • Organizer
      日本農芸化学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Methylobacterium aquaticum 22A株における Lanmodulinの機能解析2020

    • Author(s)
      藤谷良子、谷 明生
    • Organizer
      日本農芸化学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Methylobacterium aquaticum strain 22Aにおけるランタノイドスイッチのメカニズム2020

    • Author(s)
      宮本稚子、谷 明生
    • Organizer
      日本農芸化学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Lanthanide-dependent methylotrophic pathway in Methylobacterium aquaticum strain 22A2020

    • Author(s)
      Patcha Yanpirat, Akio Tani
    • Organizer
      日本農芸化学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物葉上共生細菌Methylorubrum extorquens AM1のランタノイド依存型メタノールデヒドロゲナーゼアイソザイムのレポーター遺伝子を用いた発現解析2020

    • Author(s)
      山下颯太,吉川友理, 谷 明生1,中川智行2,矢野嵩典,三井亮司
    • Organizer
      日本農芸化学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Methylobacterium aquaticum 22A株におけるランタノイドスイッチのメカニズム2019

    • Author(s)
      宮本稚子、藤谷良子、谷 明生
    • Organizer
      おかやまバイオアクティブ研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Multiple formaldehyde oxidation pathways in Methylobacterium aquaticum strain 22A2018

    • Author(s)
      Patcha Yanpirat and Akio Tani
    • Organizer
      日本微生物生態学会 第32回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Functional analysis of lanthanide inducible protein in Methylobacterium aquaticum 22A2018

    • Author(s)
      Yoshiko Fujitani and Akio Tani
    • Organizer
      日本微生物生態学会 第32回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Molecular mechanism of methylotaxis in Methylobacterium aquaticum strain 22A2018

    • Author(s)
      Yuuki Haruna, Junichi Kato, and Akio Tani
    • Organizer
      日本微生物生態学会 第32回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Methylobacterium extorquens AM1のランタノイド依存型メタノールデヒドロゲナーゼの解析2018

    • Author(s)
      吉川友理、北村純一、矢野嵩典、中川智行、谷 明生、三井亮司
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ランタノイド依存型メタノール脱水素酵素XoxF1の酵素活性と安定性はランタノイド種に依存する2018

    • Author(s)
      王 倫,菅沼宗矢,日比野歩美,谷明生,三井亮司,海老原章郎, 岩本悟志,稲垣瑞穂,島田昌也,早川享志,中川智行
    • Organizer
      農芸化学会中部支部 支部例会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ホウレンソウにおける収穫後のMethylobacterium属細菌の挙動とその成育を制御する微生物の探索2018

    • Author(s)
      阪口由佳、繁原安美、王倫、谷明生,中野浩平、稲垣瑞穂、 島田昌也、早川享志、中川智行
    • Organizer
      美味技術学会 第18回大阪例会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Formaldehyde oxidation in lanthanide-dependent methylotrophy in Methylobacterium aquaticum strain 22A2018

    • Author(s)
      Patcha Yanpirat and Akio Tani
    • Organizer
      3rd International Conference on Biologically Active Substances Bioactive Okayama 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] Functional analysis of lanthanide inducible proteins of Methylobacterium aquaticum 22A2018

    • Author(s)
      Yoshiko Fujitani and Akio Tani
    • Organizer
      3rd International Conference on Biologically Active Substances Bioactive Okayama 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] Molecular mechanism of methylotaxis in Methylobacterium aquaticum strain 22A2018

    • Author(s)
      Yuuki Haruna and Akio Tani
    • Organizer
      3rd International Conference on Biologically Active Substances Bioactive Okayama 2018
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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