Environmental-recognition of microorganisms through oligosaccharides

• Project/Area Number: 18H02132
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Kagawa University (2021); National Institute of Advanced Industrial Science and Technology (2018-2020)
• Principal Investigator: Tomohiko Matsuzawa 香川大学, 農学部, 助教 (10711971)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000); Fiscal Year 2021: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: 酵素 / 糖質 / 麹菌 / 微生物 / オリゴ糖 / 糸状菌 / 転写制御 / キシログルカン / Aspergillus oryzae / 環境応答

Outline of Final Research Achievements

Xyloglucan/xyloglucan-oligosaccharide degradation-related enzymes, such as isoprimeverose-producing oligoxyloglucan hydrolase, β-galactosidase, extracellular- and intracellular-α-xylosidases and xyloglucan-specific endo-β-1,4-glucanases, were identified and characterized in Aspergillus oryzae. The gene expressions of some of these glycoside hydrolases were induced in the presence of xyloglucan-oligosaccharides and these enzymes hydrolyzed xyloglucan/xyloglucan-oligosaccharides cooperatively.

Academic Significance and Societal Importance of the Research Achievements

麹菌は醸造産業において重要な微生物であり、多糖類やタンパク質を分解するために様々な酵素を生産している。キシログルカンは植物の細胞壁や種子に含まれている複雑な側鎖構造を有する多糖類であり、本研究では、麹菌がこのキシログルカンやキシログルカン由来オリゴ糖を分解する酵素群を明らかにした。麹菌はこれらの酵素を分解対象の基質やその分解物が存在する場合に積極的に生産し、これらの酵素を協調的に基質に作用させることによって巧みにキシログルカンを分解している。このことは、麹菌にはまだ認知されていない分解能力（酵素）とそれを引き出すためのきっかけ（誘導物質）があることを示唆している。

Research Products

• [Journal Article] Crystal structure of glycoside hydrolase family 31 α-xylosidase from a soil metagenome (2022)
  • Author(s): Nakamichi Yusuke、Matsuzawa Tomohiko、Watanabe Masahiro、Yaoi Katsuro
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: -
  • DOI: 10.1093/bbb/zbac058
  • Peer Reviewed
• [Journal Article] Characterization of an extracellular α-xylosidase involved in xyloglucan degradation in Aspergillus oryzae (2021)
  • Author(s): Matsuzawa Tomohiko、Watanabe Masahiro、Nakamichi Yusuke、Kameyama Akihiko、Kojima Naoshi、Yaoi Katsuro
  • Journal Title: Applied Microbiology and Biotechnology Volume: 106 Issue: 2 Pages: 675-687
  • DOI: 10.1007/s00253-021-11744-7
  • Peer Reviewed
• [Journal Article] Enzymatic degradation of xyloglucans by Aspergillus species: A comparative view of this genus (2021)
  • Author(s): Tomohiko Matsuzawa, Akira Watanabe, Takahiro Shintani, Katsuya Gomi, Katsuro Yaoi
  • Journal Title: Applied microbiology and biotechnology Volume: 105 Issue: 7 Pages: 2701-2711
  • DOI: 10.1007/s00253-021-11236-8
  • Peer Reviewed
• [Journal Article] Identification and characterization of two xyloglucan-specific endo-1,4-glucanases in Aspergillus oryzae (2020)
  • Author(s): Matsuzawa Tomohiko、Kameyama Akihiko、Nakamichi Yusuke、Yaoi Katsuro
  • Journal Title: Applied Microbiology and Biotechnology Volume: 104 Issue: 20 Pages: 8761-8773
  • DOI: 10.1007/s00253-020-10883-7
  • Peer Reviewed
• [Journal Article] Identification and characterization of α-xylosidase involved in xyloglucan degradation in Aspergillus oryzae (2019)
  • Author(s): Matsuzawa Tomohiko、Kameyama Akihiko、Yaoi Katsuro
  • Journal Title: Applied Microbiology and Biotechnology Volume: 104 Issue: 1 Pages: 201-210
  • DOI: 10.1007/s00253-019-10244-z
  • Peer Reviewed
• [Journal Article] Cooperation between β‐galactosidase and an isoprimeverose‐producing oligoxyloglucan hydrolase is key for xyloglucan degradation in Aspergillus oryzae (2019)
  • Author(s): Matsuzawa Tomohiko、Watanabe Masahiro、Kameda Tomoshi、Kameyama Akihiko、Yaoi Katsuro
  • Journal Title: The FEBS Journal Volume: in press Issue: 16 Pages: 3182-3193
  • DOI: 10.1111/febs.14848
  • Peer Reviewed
• [Journal Article] Crystal structure and substrate recognition mechanism of Aspergillus oryzae isoprimeverose-producing enzyme (2019)
  • Author(s): Matsuzawa Tomohiko、Watanabe Masahiro、Nakamichi Yusuke、Fujimoto Zui、Yaoi Katsuro
  • Journal Title: Journal of Structural Biology Volume: 205 Issue: 1 Pages: 84-90
  • DOI: 10.1016/j.jsb.2018.11.005
  • Peer Reviewed
• [Journal Article] A novel isoprimeverose‐producing enzyme from Phaeoacremonium minimum is active with low concentrations of xyloglucan oligosaccharides (2018)
  • Author(s): Matsuzawa Tomohiko、Kameyama Akihiko、Yaoi Katsuro
  • Journal Title: FEBS Open Bio Volume: 9 Issue: 1 Pages: 92-100
  • DOI: 10.1002/2211-5463.12549
  • Peer Reviewed / Open Access
• [Journal Article] 培養法とメタゲノム法によるユニークな新規糖質加水分解酵素の単離と解析 (2018)
  • Author(s): 松沢智彦
  • Journal Title: 応用糖質科学 Volume: 8 Pages: 206-210
  • Peer Reviewed
• [Presentation] 麹菌が生産するキシログルカンの分解に重要なα-xylosidaseの同定 (2020)
  • Author(s): 松沢智彦、亀山昭彦、矢追克郎
  • Organizer: 日本農芸化学会2020年度大会
• [Presentation] 麹菌Aspergillus oryzaeが生産するヘミセルラーゼの協調的作用 (2019)
  • Author(s): 松沢智彦、矢追克郎
  • Organizer: 第77回 日本生物工学会 大会
• [Presentation] アミノ酸配列だけではわからない糖質加水分解酵素の機能とそのシナジー (2018)
  • Author(s): 松沢智彦、矢追克郎
  • Organizer: 第18回 糸状菌分子生物学コンファレンス
• [Presentation] キシログルカンの分解に重要な麹菌由来β-ガラクトシダーゼの解析 (2018)
  • Author(s): 松沢智彦、矢追克郎
  • Organizer: 日本応用糖質科学会平成30年度大会
• [Patent(Industrial Property Rights)] 新規キシログルカナーゼ及びキシログルカンオリゴ糖の製造方法 (2020)
  • Inventor(s): 松沢智彦、亀山昭彦、矢追克郎
  • Industrial Property Rights Holder: 国立研究開発法人産業技術総合研究所
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2020-045575
  • Filing Date: 2020