| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Outline of Final Research Achievements |
Collagens are structural proteins that constitute approximately 25% of human dry body weight and are secreted from the endoplasmic reticulum to the extracellular space via the Golgi apparatus. In this study, we aimed to elucidate the molecular mechanisms by which calcium-responsive regulators, mainly the calcium-binding protein ALG-2, control export of collagens from the endoplasmic reticulum, by searching for target molecules of ALG-2 by its binding motifs and by using proximity-dependent labeling methods coupled with mass spectrometry. The protein-protein interaction networks and subcellular localization of multiple candidate regulators and their functions were elucidated by biochemical and molecular cell biological approaches.
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