| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
Aspartate kinase (AK) and homoserine dehydrogenase (HseDH) are involved in the biosynthetic pathway for L-threonine and L-lysine in plants and microorganisms. Both enzymes are important as potential targets for improving the nutritional value of food crops. AK and HseDH function as either a monofunctional enzyme or fused-bifunctional enzyme (AK-HseDH). These enzymes are highly regulated by the end products. However, information on the regulation mechanism remains limited because of their instability.
In the hyperthermophilic bacterium, Thermotoga maritima, we found presence of AK-HseDH and AK, both exhibit high stability. We revealed that the former to be an L-threonine-sensitive AK-HseDH, whose activity was not inhibited by L-lysine and the latter to be an L-lysine-sensitive AK, whose activity was not inhibited by L-threonine. Our results suggest that, in T. maritima, the AK-HseDH is related to the synthesis of L-threonine, whereas the AK functions in the synthesis of L-lysine.
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