| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Outline of Final Research Achievements |
In the current study, we analyzed the quantitative fluctuations of the storage form of ecdysteroids and the transcriptional alteration of various biosynthetic enzymes during the embryogenesis of the silkworm Bombyx mori. We aimed basically to elucidate the molecular mechanisms of endocrine regulation for the diapause in insects. We have successfully progressed two significant issues; (1) establishment of a quantitative method of the phosphorylated conjugates of ecdysteroid, a storage type of ecdysteroid using LC-MS/MS, and (2) analyses of transcriptional changes of ecdysteroid biosynthetic enzymes and metabolic enzymes throughout embryogenesis. Together, data have indicated that two distinctive 20E biosynthetic pathways, from the storage form of ecdysteroid and a biosynthetic pathway from cholesterol, could eventually produce the three differently timed 20E peaks during embryogenesis.
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