| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Outline of Final Research Achievements |
We were able to identify isoprenyl diphosphate synthases and prenyl reductase of Mycobacterium spp. Using these enzymes, we succeeded in preparing a 14C high-sensitivity substrate for the identification of Z-type sesquarterpene cyclase that contributes to the "pathway for cyclizing Z-type isoprenoids". Using this system, the native enzyme was partially purified by various chromatographies, and ZTC candidates could be narrowed down by LC-MS/MS. We also succeeded in X-ray structural analysis of the homologue of the triterpene / sesquarterpene cyclase that contributes to the "pathway that initiates cyclization from the non-terminal". The modeling structure suggested that there was a space where the substrate ends could bind near the initiation reaction site.
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