The elucidation of taste coding mechanisms based on imaging techniques

• Project/Area Number: 18H02153
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 38050:Food sciences-related
• Research Institution: The University of Tokyo
• Principal Investigator: Okada Shinji 東京大学, 大学院農学生命科学研究科(農学部), 特任准教授 (50376563)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2019: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2018: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: 味覚 / 神経 / イメージング / 可視化 / メダカ

Outline of Final Research Achievements

In this study, we have succeeded in obtaining a transcriptional regulatory region that can induce expression in medaka sour taste cells. By using this region, it is expected that the introduction of foreign genes into sour taste cells will be possible in other model fishes such as zebrafish. In addition, the trans-synaptic tracer has revealed the correlation between the conduction pathways of taste information in several species. The obtained findings are complementary to the findings of gustatory conduction pathways that have been shown in other model organisms, and will greatly contribute to the full understanding of gustatory conduction pathways. In addition to the knowledge accumulated through the examination of the conditions in this study, we expect to achieve our goal of observing the response to gustatory stimuli by utilizing tools with greatly improved fluorescence intensity and examining methods to induce the expression of these tools.

Academic Significance and Societal Importance of the Research Achievements

本申請研究では、複数種の味細胞に発現誘導可能な転写制御領域の取得に成功した。得られた転写制御領域の活用は味覚受容、味覚情報伝達などの研究の進展に大きく貢献するものである。また、申請者のグループの本申請研究およびこれまでの研究によって、5基本味の味覚情報伝導路の重なり合いの様式が明らかになってきた。なぜそれぞれの味が認識できるのか、その仕組みの解明に貢献するものである。

Research Products

• [Journal Article] Dietary iso-α-acids prevent acetaldehyde-induced liver injury through Nrf2-mediated gene expression (2021)
  • Author(s): Takase, T., Toyoda, T., Kobayashi, N., Inoue, T., Ishijima, T., Abe, K., Kinoshita, H., Tsuchiya, Y., and Okada, S.
  • Journal Title: PLoS One Volume: 16 Issue: 2 Pages: e0246327-e0246327
  • DOI: 10.1371/journal.pone.0246327
  • Peer Reviewed / Open Access
• [Journal Article] Changes in Whole-Blood microRNA Profiles during the Onset and Treatment Process of Cerebral Infarction: A Human Study (2020)
  • Author(s): Abe Arata、Tanaka Mayui、Yasuoka Akihito、Saito Yoshikazu、Okada Shinji、Mishina Masahiro、Abe Keiko、Kimura Kazumi、Asakura Tomiko
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Issue: 9 Pages: 3107-3107
  • DOI: 10.3390/ijms21093107
  • Peer Reviewed / Open Access
• [Journal Article] Tyr-Trp administration facilitates brain norepinephrine metabolism and ameliorates a short-term memory deficit in a mouse model of Alzheimer’s disease. (2020)
  • Author(s): Ichinose, T., Murasawa, H., Ishijima, T., Okada, S., Abe, K., Matsumoto, S., Matsui, T., and Furuya, S.
  • Journal Title: PLoS One Volume: 15 Issue: 5 Pages: e0232233-e0232233
  • DOI: 10.1371/journal.pone.0232233
  • Peer Reviewed / Open Access
• [Journal Article] Dietary Intake of Carotenoid-Rich Vegetables Reduces Visceral Adiposity in Obese Japanese men-A Randomized, Double-Blind Trial. (2020)
  • Author(s): Takagi T., Hayashi R., Nakai Y., Okada S., Miyashita R., Yamada M., Mihara Y., Mizushima K., Morita M., Uchiyama K., Naito Y., and Itoh Y.
  • Journal Title: Nutrients Volume: 12 Issue: 8 Pages: 2342-2342
  • DOI: 10.3390/nu12082342
  • Peer Reviewed / Open Access
• [Journal Article] A maple syrup extract alleviates liver injury in type 2 diabetic model mice. (2020)
  • Author(s): Toyoda, T., Iida, K., Ishijima, T., Abe, K., Okada, S., and Nakai, Y.
  • Journal Title: Nutr. Res. Volume: 73 Pages: 97-101
  • DOI: 10.1016/j.nutres.2019.10.006
  • Peer Reviewed / Open Access
• [Journal Article] Intake of sake-cake and rice-koji mixture improves intestinal barrier function in mouse (2019)
  • Author(s): Kawakami,S., Ito, R., Maruki-Uchida, H., Kamei, A., Yasuoka, A., Toyoda,T., Ishijima, T., Nishimura, E., Morita, M., Sai, M., Abe, K., and Okada, S.
  • Journal Title: Nutrients Volume: 12 Issue: 2 Pages: 449-449
  • DOI: 10.3390/nu12020449
  • Peer Reviewed / Open Access
• [Journal Article] A maple syrup extract alters lipid metabolism in obese type 2 diabetic model mice. (2019)
  • Author(s): Toyoda,T., Kamei,A., Ishijima,T., Abe,K., and Okada,S.
  • Journal Title: Nutrition and metabolism Volume: 16 Issue: 1 Pages: 84-84
  • DOI: 10.1186/s12986-019-0403-2
  • Peer Reviewed / Open Access
• [Journal Article] Dietary Alaska pollack protein improves skeletal muscle weight recovery after immobilization-induced atrophy in rats (2019)
  • Author(s): Fujitani Mina、Mizushige Takafumi、Kawabata Fuminori、Uozumi Keisuke、Yasui Machi、Hayamizu Kohsuke、Uchida Kenji、Okada Shinji、Keshab Bhattarai、Kishida Taro
  • Journal Title: PLOS ONE Volume: 14 Issue: 6 Pages: e0217917-e0217917
  • DOI: 10.1371/journal.pone.0217917
  • Peer Reviewed / Open Access
• [Presentation] 量分析イメージングを用いたスケソウダラタンパク質摂餌ラットの肥大骨格筋解析 (2020)
  • Author(s): 森笹 瑞季、木村 圭佑、藤谷 美菜、岸田 太郎、岡田 晋治、吉田 恵里子、内田 健志、森 司、井上 菜穂子
  • Organizer: 第74回日本栄養・食糧学会大会
• [Presentation] スケソウダラ魚肉タンパク質摂取による骨格筋量増大作用の機序解明 (2020)
  • Author(s): 山本 衿子、藤谷 美菜、内田 健志、井上 菜穂子、阿部 啓子、岸田 太郎、岡田 晋治
  • Organizer: 日本農芸化学会2020年度大会
• [Presentation] イソα酸のNrf2を介したアルコール性肝障害予防効果 (2020)
  • Author(s): 高瀬 貴仁、豊田 集、小林 直之、井上 孝司、石島 智子、土屋 陽一、岡田 晋治
  • Organizer: 日本農芸化学会2020年度大会
• [Presentation] 糖尿病がアルツハイマー病態に与える影響とロスマリン酸の効果の検証 (2020)
  • Author(s): 宮脇 里奈、山下 玲、伯野 史彦、高橋 伸一郎、岡田 晋治、小林 彰子
  • Organizer: 日本農芸化学会2020年度大会
• [Presentation] Resistant starch promotes equol production and improves polycystic ovary syndrome (PCOS) symptoms in rats treated with soy isoflavones. (2019)
  • Author(s): Liyanage, S.G., Ishijima, T., Inoue, R., Abe, K., and Okada, S.
  • Organizer: ACN2019 (13th Asian Conference of Nutrition)
  • Int'l Joint Research
• [Presentation] Efficacy of Partially Hydrolyzed Guar Gum on high fat diet-induced metabolic syndrome in mice. (2019)
  • Author(s): Abe, A., Abe, K., Inoue, R., Naito, Y., Yasukawa, Z., Ozeki, M., Okubo, T., and Okada, S.
  • Organizer: The 7th International Conference on Food Factors (ICoFF2019) / The 12thInternational Conference and Exhibition on Nutraceuticals and Functional Foods 2019 (ISNFF2019)
  • Int'l Joint Research
• [Presentation] Effects of soy isoflavones on alleviation of polycystic ovary syndrome (PCOS). (2019)
  • Author(s): Liyanage, S.G., Ishijima, T., Inoue, R., Abe, K., and Okada, S.
  • Organizer: ISAPP-SFA 2019 meeting
  • Int'l Joint Research
• [Presentation] スケトウダラタンパク質摂取による骨格筋肥大効果 (2019)
  • Author(s): 藤谷 美菜、内田 健志、水重 貴文、上東 亮太、土居 直史、中川 日向太、吉田 恵里子、速水 耕介、岡田 晋治、岸田 太郎
  • Organizer: 第6回 日本サルコペニア・フレイル学会大会
• [Presentation] スケトウダラタンパク質摂取によるラット骨格筋重量増加効果は長期間持続する (2019)
  • Author(s): 中川 日向太、上東 亮太、ケシャブ バッタライ、井上 元輝、内田 健志、吉田 恵里子、土居 直史、藤谷 美菜、速水 耕介、岡田 晋治、岸田 太郎
  • Organizer: 第52回 日本栄養・食糧学会 中国・四国支部大会
• [Presentation] スケトウダラタンパク質によるラット骨格筋重量増加効果は長期間に渡って維持される (2019)
  • Author(s): 上東 亮太、吉田 恵里子、ケシャブ バッタライ、井上 元輝、土居 直史、中川 日向太、藤谷 美菜、内田 健志、速水 耕介、岡田 晋治、岸田 太郎
  • Organizer: 日本アミノ酸学会 第13回学術大会
• [Presentation] 質量分析イメージングを用いたスケソウダラタンパク質摂餌ラットの肥大骨格筋解析 (2019)
  • Author(s): 森笹 瑞季、井上 菜穂子、木村 圭佑、岸田 太郎、藤谷 美菜、内田 健志、吉田 恵里子、岡田 晋治、森 司
  • Organizer: 日本アミノ酸学会 第13回学術大会
• [Remarks] 食品機能学寄付講座
  • URL: https://park.itc.u-tokyo.ac.jp/foodfunc/
• [Remarks] 食品機能学寄付講座
  • URL: https://webpark2079.sakura.ne.jp/wp/