Eatablishment and molecular mechanism of antiinflammatory action of vitamin C by using ODS rats.
Project/Area Number |
18H02155
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 38050:Food sciences-related
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Research Institution | Nagoya University |
Principal Investigator |
Horio Fumihiko 名古屋大学, 生命農学研究科, 教授 (20165591)
|
Co-Investigator(Kenkyū-buntansha) |
大野 民生 名古屋大学, 医学系研究科, 准教授 (90293620)
|
Project Period (FY) |
2018-04-01 – 2021-03-31
|
Project Status |
Completed (Fiscal Year 2020)
|
Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
|
Keywords | ビタミンC / 抗炎症作用 / 炎症性サイトカイン / エンドトキシン / ODSラット |
Outline of Final Research Achievements |
In ODS rats unable to synthesize vitamin C (VC), we have demonstrated that VC deficiency elevates serum concentration of C-reactive protein (CRP) and stimulate hepatic CRP gene expression. As CRP is an inflammatory marker, VC deficiency causes inflammatory changes in liver. To examine the possibility that enterobacterium endotoxin incorporated into blood stream may stimulate hepatic CRP expression, we investigated in this study whether VC deficiency caused the stimulation of hepatic CRP expression or not in germ-free ODS rats. As a result, VC deficiency significantly provoked the stimulation of hepatic CRP expression in germ-free (endotoxin-free) ODS rats. Moreover, we clarified that VC deficiency induced the intestinal interleukin-6 production and suggested that interleukin-6 produced in the intestine stimulated the hepatic expression of CRP in VC deficiency.
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Academic Significance and Societal Importance of the Research Achievements |
VC欠乏による炎症性マーカーであるCRPの発現上昇と血中濃度上昇はODSラットとヒトとで共通したVCの生理作用であり、この現象が起こる機構において腸内細菌が介在しないことを本研究で初めて明らかにできた。本研究は無菌動物においてVCの生理機能を探求した国内外で初めての例であり、VCの生理機能の新たな側面を発掘し学術的意義は大きい。さらに、VC欠乏は腸管(空腸や回腸)に鋭敏に作用して炎症性サイトカインの産生を誘発することが初めて明らかとなり、腸管での炎症を抑えるためにはVCの十分な摂取が有効であるという社会的な波及効果がある。
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Ascorbic acid deficiency induces hepatic and intesitinal expression of inflammation-related genes irrespective of the presence or absence of gut microbiota in ODS rats.2020
Author(s)
Kawade, N., Murai, A., Suzuki, W., Takeuchi, K., Kondo, M., Kobayashi, M. and Horio, F.
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Journal Title
J.Nutr.Biochem.
Volume: 86
Pages: 108485-108485
DOI
Related Report
Peer Reviewed
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[Journal Article] Ablation of Iah1, a candidate gene for diet-induced fatty liver, does not affect liver lipid accumulation in mice.2020
Author(s)
Masuya, T., Suzuki, M., Tsujimura, J., Kanamori, S., Miyasaka, Y., Ohno, T., Murai, A., Horio, F. and Kobayashi, M.
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Journal Title
PLOS ONE
Volume: 15
Issue: 5
Pages: e0233087-e0233087
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Deficiency of ascorbic acid decreases the contents of tetrahydrobiopterin in the liver and the brain of ODS rats.2020
Author(s)
Takeshita, N., Kawade, N., Suzuki, W., Hara, S., Horio, F. and Ichinose, H.
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Journal Title
Neurosci Lett.
Volume: 715
Pages: 134656-134656
DOI
Related Report
Peer Reviewed
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[Journal Article] Ascorbic acid deficiency increaseshepatic expression of acute phase proteins through the intestine-derived IL-6 and hepatic STST3 pathway in ODS rats.2019
Author(s)
Kawade, N., Murai, A., Suzuki, W., Tokuda, Y., Kobayashi, M. and Horio, F.
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Journal Title
J.Nutr.Biochem.
Volume: 70
Pages: 116-124
Related Report
Peer Reviewed
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[Journal Article] Dietary Intake of Ascorbic Acid Attenuates Lipopolysaccharide-Induced Sepsis and Septic Inflammation in ODS Rat.2018
Author(s)
Kawade, N., Tokuda, Y., Tsujino, S., Aoyama, H., Kobayashi, M., Murai, A. and Horio, H.
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Journal Title
J.Nutr.Sci.Vitaminol.
Volume: 64
Pages: 404-411
NAID
Related Report
Peer Reviewed
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