Studies on pathogen recognition and functional expression for plant immunity mediated by ubiquitin modification

• Project/Area Number: 18H02162
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 38060:Applied molecular and cellular biology-related
• Research Institution: Hokkaido University
• Principal Investigator: Yamaguchi Junji 北海道大学, 理学研究院, 教授 (10183120)
• Co-Investigator(Kenkyū-buntansha): 佐藤 長緒 北海道大学, 理学研究院, 准教授 (50609724)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2018: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: ユビキチンリガーゼ / 植物免疫 / 病原体認識受容体 / 膜交通 / 植物 / 幕交通

Outline of Final Research Achievements

In this study, we focused on the function of ATL31, a membrane-localized ubiquitin ligase conserved in plants, and investigated the signal transduction mechanism regulating pathogen resistance in plants. Our biochemical analyzes demonstrated that ATL31’s ubiquitin ligase activity was changed in response to MAMPs. We also found the specific ubiquitin chain type signals are involved in the ATL31-mediating pathogen attack signals in plan cells.

Academic Significance and Societal Importance of the Research Achievements

植物の病原体抵抗性（植物免疫）は，農作物の収量向上を実現する上で重要な植物生理現象である。本研究で，これまで分かっていなかった植物免疫におけるユビキチンシグナルの関与が示された。本研究成果は，植物免疫を制御する分子基盤の理解や複数の環境ストレスに対する植物の適応機構の解明に貢献すると期待される。

Research Products

• [Int'l Joint Research] Max Planck Institute/University of Hohenheim(ドイツ)
• [Int'l Joint Research] Max Planck Institute/University of Hohenheim/Molecular Plant hysiology(ドイツ)
• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Journal Article] Deubiquitinating enzymes UBP12 and UBP13 stabilize the brassinosteroid receptor BRI1 (2022)
  • Author(s): Luo Yongming、Takagi Junpei、Claus Lucas Alves Neubus、Zhang Chao、Yasuda Shigetaka、Hasegawa Yoko、Yamaguchi Junji、Shan Libo、Russinova Eugenia、Sato Takeo
  • Journal Title: EMBO reports Volume: 23 Issue: 4
  • DOI: 10.15252/embr.202153354
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The TGN/EE SNARE protein SYP61 and the ubiquitin ligase ATL31 cooperatively regulate plant responses to carbon/nitrogen conditions in Arabidopsis (2022)
  • Author(s): Yoko Hasegawa, Thais Huarancca Reyes, Tomohiro Uemura, Anirban Baral, Akari Fujimaki, Yongming Luo, Yoshie Morita, Yasushi Saeki, Shugo Maekawa, Shigetaka Yasuda, Koki Mukuta, Yoichiro Fukao, Keiji Tanaka, Akihiko Nakano, Junpei Takagi, Rishikesh P Bhalerao, Junji Yamaguchi, Takeo Sato
  • Journal Title: THE PLANT CELL Volume: 34 Issue: 4 Pages: 1354-1374
  • DOI: 10.1093/plcell/koac014
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 3-Phenyllactic acid, a root-promoting substance isolated from Bokashi fertilizer, exhibits synergistic effects with tryptophan (2021)
  • Author(s): Maki Yuko、Soejima Hiroshi、Kitamura Toru、Sugiyama Tamizi、Sato Takeo、Watahiki Masaaki K.、Yamaguchi Junji
  • Journal Title: Plant Biotechnology Volume: 38 Issue: 1 Pages: 9-16
  • DOI: 10.5511/plantbiotechnology.20.0727a
  • NAID: 130008002853
  • ISSN: 1342-4580, 1347-6114
  • Year and Date: 2021-03-25
  • Peer Reviewed
• [Journal Article] Effect of circularly polarized light on germination, hypocotyl elongation and biomass production of arabidopsis and lettuce: Involvement of phytochrome B (2020)
  • Author(s): Lkhamkhuu Enkhsukh、Zikihara Kazunori、Katsura Hitomi、Tokutomi Satoru、Hosokawa Takafumi、Usami Yoshihisa、Ichihashi Mitsuyoshi、Yamaguchi Junji、Monde Kenji
  • Journal Title: Plant Biotechnology Volume: 37 Issue: 1 Pages: 57-67
  • DOI: 10.5511/plantbiotechnology.19.1219a
  • NAID: 130007822566
  • ISSN: 1342-4580, 1347-6114
  • Year and Date: 2020-03-25
  • Peer Reviewed
• [Journal Article] Protein Phosphorylation Dynamics Under Carbon/Nitrogen-Nutrient Stress and Identification of a Cell Death-Related Receptor-Like Kinase in Arabidopsis (2020)
  • Author(s): Li Xingwen、Sanagi Miho、Lu Yu、Nomura Yuko、Stolze Sara Christina、Yasuda Shigetaka、Saijo Yusuke、Schulze Waltraud X.、Feil Regina、Stitt Mark、Lunn John E.、Nakagami Hirofumi、Sato Takeo、Yamaguchi Junji
  • Journal Title: Frontiers in Plant Science Volume: 11 Pages: 377-377
  • DOI: 10.3389/fpls.2020.00377
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Involvement of the membrane-localized ubiquitin ligase ATL8 in sugar starvation response in Arabidopsis (2019)
  • Author(s): Luo Yongming、Aoyama Shoki、Fukao Yoichiro、Chiba Yukako、Sato Takeo、Yamaguchi Junji
  • Journal Title: Plant Biotechnology Volume: 36 Issue: 2 Pages: 107-112
  • DOI: 10.5511/plantbiotechnology.19.0328a
  • NAID: 130007672660
  • ISSN: 1342-4580, 1347-6114
  • Year and Date: 2019-06-25
  • Peer Reviewed
• [Journal Article] Identification of Arabidopsis CCR4-NOT Complexes with Pumilio RNA-Binding Proteins, APUM5 and APUM2 (2019)
  • Author(s): Arae Toshihiro、Morita Kotone、Imahori Riko、Suzuki Yuya、Yasuda Shigetaka、Sato Takeo、Yamaguchi Junji、Chiba Yukako
  • Journal Title: Plant and Cell Physiology Volume: 60 Issue: 9 Pages: 2015-2025
  • DOI: 10.1093/pcp/pcz089
  • Peer Reviewed
• [Presentation] Deubiquitinating enzymes limit the degradation of brassinosteroid receptor BRI1 in Arabidopsis (2022)
  • Author(s): Luo Y, Takagi J, Claus LAN, Zhang C, Yasuda S, Hasegawa Y, Yamaguchi J, Shan L, Russinova E and Sato T
  • Organizer: 第63回日本植物生理学会年会
• [Presentation] SNAREタンパク質SYP61はユビキチンリガーゼATL31と共にシロイヌナズナのC/N栄養応答を制御する (2022)
  • Author(s): 2.長谷川陽子，Thais Huarancca Reyes, 植村知博，Anirban Baral, 羅永茗, 前川修吾, 安田盛貴, 深尾陽一朗, 中野明彦，高木純平，Rishikesh P. Bhalerao, 山口淳二，佐藤 長緒
  • Organizer: 第63回日本植物生理学会年会
• [Book] Proteomic analysis of 14-3-3 targets in tomato fruit tissues. In “Plant Proteomics: Methods and Protocol, Third Edition” (2020)
  • Author(s): Luo Y, Lu Y, Yamaguchi J, Sato T
  • Total Pages: 8
  • Publisher: Springer Protocol, Humana, New York, NY
  • ISBN: 9781071605271
• [Book] 植物に対する乳酸菌培養液の機能性，最新農業技術『土壌施肥』 第12巻，<環境ストレスに強くするバイオスティミュラント> (2020)
  • Author(s): 眞木祐子，山口淳二
  • Total Pages: 3
  • Publisher: 農山漁村文化協会
• [Book] Chapter 21, Proteomic analysis of 14-3-3 targets in tomato fruit tissues. In “Plant Proteomics: Methods and Protocol, Third Edition” (edited by J.V. Jorr&iacute;n Novo, L. Valledor, and M.A. Castillejo) Methods in Molecular Biology, vol. 2039 (2020)
  • Author(s): Luo Y, Lu Y, Yamaguchi J, Sato T
  • Publisher: Springer Protocol, Humana, New York, NY
  • ISBN: 9781071605271
• [Remarks] 北海道大学大学院理学研究院形態機能学講座Ⅱ
  • URL: https://www.sci.hokudai.ac.jp/CSF2-web/
• [Remarks] 山口 淳二 教授 YAMAGUCHI Junji
  • URL: https://www2.sci.hokudai.ac.jp/faculty/researcher/junji-yamaguchi
• [Patent(Industrial Property Rights)] 不定根発生誘導剤及び根系発達促進剤 (2020)
  • Inventor(s): 眞木裕子，副島洋，山口淳二，谷野圭持，綿引雅昭，佐藤長緒
  • Industrial Property Rights Holder: 眞木裕子，副島洋，山口淳二，谷野圭持，綿引雅昭，佐藤長緒
  • Industrial Property Rights Type: 特許
  • Filing Date: 2020
  • Acquisition Date: 2020
• [Patent(Industrial Property Rights)] 不定根発生誘導剤及び根系発達促進剤 (2020)
  • Inventor(s): 眞木裕子，副島洋，山口淳二，綿引雅昭，佐藤長緒
  • Industrial Property Rights Holder: 眞木裕子，副島洋，山口淳二，綿引雅昭，佐藤長緒
  • Industrial Property Rights Type: 特許
  • Filing Date: 2020
  • Acquisition Date: 2020