| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2022: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2021: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Outline of Final Research Achievements |
Based on the "relationship between RsgA and RbfA" that I have clarified so far, I focused four types of maturation factors (RsgA, RbfA, RimM, Era) required for the late stage of the bacterial ribosomal 30S subunit biosynthesis to analyze the role of each factor on 30S subunit maturation. In addition, by conducting structural analysis of biosynthetic intermediates, we analyzed the molecular mechanisms in detail, leading to better understanding of the maturation process of the ribosome. We will establish a system for in vitro assembly of the mature 30S subunits from 30S subunit biosynthetic intermediates accumulated in maturation factor-deficient cells, and use this as a basis for the development of novel antibiotics.
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