Analysis of roles of nuclear actin family in the maintenance of genome stability and operation of their functions using peptides
| Project/Area Number |
18H02164
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 38060:Applied molecular and cellular biology-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
太田 邦史 東京大学, 大学院総合文化研究科, 教授 (90211789)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | 細胞核 / クロマチン / ヒストンバリアント / アクチンファミリー / ヒストン / 核内アクチン / ヒストンバリアントH2A.Z / アクチン / エピジェネティクス / bicyclic peptide |
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| Outline of Final Research Achievements |
The histone variant H2A.Z functions in the regulation of gene expression through its deposition and eviction. However, the mechanisms of the molecular function of H2A.Z was largely unknown. In this study, we showed the relationship between H2A.Z function and nuclear actin family proteins by using knockout cells for H2A.Z and actin family proteins. In addition, by using bicyclic peptides for Arp6 and Arp8, we identified molecular mechanisms regulating deposition and eviction of H2A.Z.
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| Academic Significance and Societal Importance of the Research Achievements |
H2A/Zノックアウト細胞や、細胞核内アクチンファミリーに結合する二重環状ペプチドを用いた解析により、H2A.Zの導入や排除を行う複合体の機能メカニズムの一端を明らかにすることができた。この結果は、アクチンファミリーに結合する二重環状ペプチドによって、H2A.Zダイナミクスを介した人為的な遺伝子機能解析を行うことができる可能性を示唆しており、今後の農学・医学領域への応用も期待される。
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Report
(4 results)
Research Products
(89 results)
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[Journal Article] In Vitro-Evolved Peptides Bind Monomeric Actin and Mimic Actin-Binding Protein Thymosin-beta42021
Author(s)
Gubeli RJ, Bertoldo D, Shimada K, Gerhold CB, Hurst V, Takahashi Y, Harada K, Mothukuri GK, Wilbs J, Harata M, Gasser SM, Heinis C.
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Journal Title
ACS Chem Biol
Volume: 16
Issue: 5
Pages: 820-828
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Distinct roles of ATM and ATR in the regulation of ARP8 phosphorylation to prevent chromosome translocations2018
Author(s)
Sun, J., Shi, L., Kinomura, A., Fukuto, A., Horikoshi, Y., Oma, Y., Harata, M., Ikura, M., Ikura, T., Kanaar, R., Tashiro, S
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Journal Title
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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