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Structure and function of membrane microdomains in plant environmental stress response

Research Project

Project/Area Number 18H02165
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38060:Applied molecular and cellular biology-related
Research InstitutionSaitama University

Principal Investigator

Maki Kawai-Yamada  埼玉大学, 理工学研究科, 教授 (10332595)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywordsスフィンゴ脂質 / シロイヌナズナ / 不飽和化 / 環境ストレス / 細胞膜 / 低温応答 / 糖鎖 / マイクロドメイン / ストレス応答 / 環境ストレス応答
Outline of Final Research Achievements

Plasma membrane is the place where plant cells receive signals from the outside and elicit intracellular responses. Various proteins such as receptors and channels are reported to localize on the cell membrane. The sphingolipid- and sterol- enriched functional microdomains, known as lipid raft, is a universal organization principle for cellular membranes in plants. To elucidate the biological function of plant microdomains in environmental stress responses, we analyzed the sphingolipid synthetic pathway of Arabidopsis thaliana, a model plant. Studies on T-DNA insertion mutants for sphingolipid-related genes demonstrated that desaturation of sphingolipids is essential for the cold response of Arabidopsis.

Academic Significance and Societal Importance of the Research Achievements

動物では、脂質ラフトの異常と疾患との関係性が早くより示され、国内外で盛んに研究が進められてきたのに対し、植物の脂質ラフトに関する知見は乏しい。動物のラフトは主にスフィンゴミエリンとコレステロール等の脂質成分から構成される。しかし、植物にはこれらの脂質成分がほとんど存在せず、その構造や機能は不明であった。本研究では、モデル植物であるシロイヌナズナを用い、植物の脂質ラフトを構成するスフィンゴ脂質の合成系と、その機能の一端を明らかにした。また、本研究により、脂質ラフトの機能が植物の低温応答に必要であることが示さた。これらの結果は、今後、低温耐性を有する作物の分子育種の基盤となる知見である。

Report

(5 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (50 results)

All 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (14 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 14 results,  Open Access: 14 results) Presentation (33 results) (of which Int'l Joint Research: 6 results)

  • [Int'l Joint Research] Lawrence Berkeley National Laboratory(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] ボルドー大学(フランス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Lawrence Berkeley National laboratory(米国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Sphingolipids with 2-hydroxy fatty acids aid in plasma membrane nanodomain organization and oxidative burst2022

    • Author(s)
      Ukawa Tomomi、Banno Fumihiko、Ishikawa Toshiki、Kasahara Kota、Nishina Yuuta、Inoue Rika、Tsujii Keigo、Yamaguchi Masatoshi、Takahashi Takuya、Fukao Yoichiro、Kawai-Yamada Maki、Nagano Minoru
    • Journal Title

      Plant Physiology

      Volume: - Issue: 2 Pages: 839-857

    • DOI

      10.1093/plphys/kiac134

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ceramides mediate positional signals in Arabidopsis thaliana protoderm differentiation2021

    • Author(s)
      Nagata Kenji、Ishikawa Toshiki、Kawai-Yamada Maki、Takahashi Taku、Abe Mitsutomo
    • Journal Title

      Development

      Volume: 148 Issue: 2 Pages: 194969-194969

    • DOI

      10.1242/dev.194969

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Arabidopsis thaliana nucleotide sugar transporter GONST2 is a functional homolog of GONST12021

    • Author(s)
      Jing Beibei、Ishikawa Toshiki、Soltis Nicole、Inada Noriko、Liang Yan、Murawska Gosia、Fang Lin、Andeberhan Fekadu、Pidatala Ramana、Yu Xiaolan、Baidoo Edward、Kawai‐Yamada Maki、Loque Dominique、Kliebenstein Daniel J.、Dupree Paul、Mortimer Jenny C.
    • Journal Title

      Plant Direct

      Volume: 5 Issue: 3

    • DOI

      10.1002/pld3.309

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Generation of Arabidopsis lines with a red fluorescent marker for endoplasmic reticulum using a tail-anchored protein cytochrome b5 -B2020

    • Author(s)
      Nagano Minoru、Ueda Haruko、Fukao Yoichiro、Kawai-Yamada Maki、Hara-Nishimura Ikuko
    • Journal Title

      Plant Signaling & Behavior

      Volume: 15 Issue: 9 Pages: 1790196-1790196

    • DOI

      10.1080/15592324.2020.1790196

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Altered metabolism of chloroplastic NAD kinase-overexpressing Arabidopsis in response to magnesium sulfate supplementation2020

    • Author(s)
      Kawai-Yamada Maki、Miyagi Atsuko、Sato Yuki、Hosoi Yuki、Hashida Shin-Nosuke、Ishikawa Toshiki、Yamaguchi Masatoshi
    • Journal Title

      Plant Signaling & Behavior

      Volume: 16 Issue: 1 Pages: 1844509-1844509

    • DOI

      10.1080/15592324.2020.1844509

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dehydroascorbate Reductases and Glutathione Set a Threshold for High-Light?Induced Ascorbate Accumulation2020

    • Author(s)
      Terai Yusuke、Ueno Hiromi、Ogawa Takahisa、Sawa Yoshihiro、Miyagi Atsuko、Kawai-Yamada Maki、Ishikawa Takahiro、Maruta Takanori
    • Journal Title

      Plant Physiology

      Volume: 183 Issue: 1 Pages: 112-122

    • DOI

      10.1104/pp.19.01556

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Plant-Unique cis/trans Isomerism of Long-Chain Base Unsaturation is Selectively Required for Aluminum Tolerance Resulting from Glucosylceramide-Dependent Plasma Membrane Fluidity2020

    • Author(s)
      Sato Masaya、Nagano Minoru、Jin Song、Miyagi Atsuko、Yamaguchi Masatoshi、Kawai-Yamada Maki、Ishikawa Toshiki
    • Journal Title

      Plants

      Volume: 9 Issue: 1 Pages: 19-19

    • DOI

      10.3390/plants9010019

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oxalate contents in leaves of two rice cultivars grown at a free-air CO<sub>2</sub> enrichment (FACE) site2019

    • Author(s)
      Atsuko Miyagi, Ko Noguchi, Takeshi Tokida, Yasuhiro Usui, Hirofumi Nakamura, Hidemitsu Sakai, Toshihiro Hasegawa, Maki Kawai-Yamada
    • Journal Title

      Plant Production Science

      Volume: - Issue: 3 Pages: 407-411

    • DOI

      10.1080/1343943x.2019.1598272

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High-yielding rice Takanari has superior photosynthetic response to a commercial rice Koshihikari under fluctuating light2019

    • Author(s)
      Adachi Shunsuke、Tanaka Yu、Miyagi Atsuko、Kashima Makoto、Tezuka Ayumi、Toya Yoshihiro、Kobayashi Shunzo、Ohkubo Satoshi、Shimizu Hiroshi、Kawai-Yamada Maki、Sage Rowan F、Nagano Atsushi J、Yamori Wataru
    • Journal Title

      Journal of Experimental Botany

      Volume: 70 Issue: 19 Pages: 5287-5297

    • DOI

      10.1093/jxb/erz304

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The mitochondrial alternative oxidase supports redox balance of the photosynthetic electron transport, primary metabolite balance and growth at high light.2019

    • Author(s)
      Zhenxiang Jiang, Chihiro K.A. Watanabe, Atsuko Miyagi, Maki Kawai-Yamada, Ichiro Terashima, Ko Noguchi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 20 Issue: 12 Pages: 3067-3067

    • DOI

      10.3390/ijms20123067

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Glycosylinositol phosphoceramide-specific phospholipase D activity catalyzes transphosphatidylation2019

    • Author(s)
      Rumana Yesmin Hasi, Makoto Miyagi, Katsuya Morito, Toshiki Ishikawa, Maki Kawai-Yamada, Hiroyuki Imai, Tatsuya Fukuta Kentaro Kogure Kaori Kanemaru, Junji Hayashi, Ryushi Kawakami and Tamotsu Tanaka
    • Journal Title

      J. Biochem.

      Volume: 166 Issue: 5 Pages: 441-448

    • DOI

      10.1093/jb/mvz056

    • NAID

      120006879927

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] <i>DSH5</i>, a dihydrosphingosine C4 hydroxylase gene family member, shows spatially restricted expression in rice and is lethal when expressed ectopically2018

    • Author(s)
      Imamura T, Obata C, Yoneyama K, Ichikawa M, Ikura A, Mutsuro-Aoki H, Ishikawa T, Kawai-Yamada M, Sasaki T, Kusano H, Shimada H.
    • Journal Title

      Genes & Genetic Systems

      Volume: 93 Issue: 4 Pages: 135-142

    • DOI

      10.1266/ggs.17-00054

    • NAID

      130007503884

    • ISSN
      1341-7568, 1880-5779
    • Year and Date
      2018-08-01
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) is a GlcNAc-containing glycosylinositol phosphorylceramide glycosyltransferase.2018

    • Author(s)
      Ishikawa T, Fang L, Rennie EA, Sechet J, Yan J, Jing B, Moore W, Cahoon EB, Scheller HV, Kawai-Yamada M, Mortimer JC.
    • Journal Title

      Plant Physiol.

      Volume: 177 Issue: 3 Pages: 938-952

    • DOI

      10.1104/pp.18.00396

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Suppressing Arabidopsis GGLT1 affects growth by reducing the L-galactose content and borate cross-linking of rhamnogalacturonan II2018

    • Author(s)
      Sechet J, Htwe S, Urbanowicz B, Agyeman A, Feng W, Ishikawa T, Dinneny J, Colomes M, Satish KK, Kawai-Yamada M, O'Neill M, Mortimer J
    • Journal Title

      The Plant Journal

      Volume: 96 Issue: 5 Pages: 1036-1050

    • DOI

      10.1111/tpj.14088

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 有用セラミト生産法の開発に向けたスフィンゴ糖脂質糖転移酵素欠損変異体の解析2022

    • Author(s)
      鈴木雄介、宮城敦子、山口雅利、川合真紀、石川寿樹
    • Organizer
      第39回日本植物バイオテクノロジー学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] タハコ一過発現系を用いたスフィンゴ脂質分解酵素遺伝子の探索2022

    • Author(s)
      真田昇、Rumana Yesmin Hasi、田中保、今井博之、宮城敦子、山口雅利、川合真紀、石川寿樹
    • Organizer
      第39回日本植物バイオテクノロジー学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 油脂産生微細藻類NannochloropsisのNAD(P)(H)合成経路の解析2022

    • Author(s)
      林英里香、鈴木耕陽、宮城敦子、石川寿樹、山口雅利、川合真紀
    • Organizer
      植物脂質科学シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] 植物スフィンゴ脂質GIPCを分解するホスホリパーゼの探索2022

    • Author(s)
      真田昇、近藤雄大、Rumana Yesmin Hasi、田中保、今井博之、山口雅利、川合真紀、石川寿樹
    • Organizer
      植物脂質科学シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] Heterologous complementation analysis reveals a distinct function of two glycosphingolipid subclasses in Arabidopsis2021

    • Author(s)
      Yamato Kudo, Atsuko Miyagi, Masatoshi Yamaguchi, Maki Kawai-Yamada, Toshiki Ishikawa
    • Organizer
      第62回植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Analyses of transcriptional regulation of cuticular wax accumulation in response to dehydration2021

    • Author(s)
      浦野薫、圓山恭之進、大島良美、坂本真吾、石川寿樹、川合真紀、佐藤繭子、豊岡公徳、篠崎和子、篠崎一雄
    • Organizer
      第62回植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] スフィンゴ脂質糖鎖改変シロイヌナズナの表現型の解析2020

    • Author(s)
      工藤大和、宮城敦子、山口雅利、川合真紀、石川寿樹
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物のクチクラ形成を調節する乾燥応答ネットワークの解析2020

    • Author(s)
      浦野薫、圓山恭之進、大島良美、坂本真吾、石川寿樹、佐藤繭子、豊岡公徳、川合真紀、篠崎和子、篠崎 一雄
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イネのカマイラズ変異体のメタボローム解析2020

    • Author(s)
      森和久、宮城敦子、石川寿樹、山口雅利、小竹敬久、川合真紀
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Alteration of oxalate content in rice leaves obtained from ion beam-irradiated plants2020

    • Author(s)
      Atsuko Miyagi, Takuya Saimaru, Nozomi Harigai, Yutaka Oono, Yoshihiro Hase, Maki Kawai-Yamada
    • Organizer
      Plant Biology 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] コケ植物独自のスフィンゴ脂質構造を形成する長鎖塩基Δ8不飽和化酵素の機能解析2019

    • Author(s)
      小川洋祐、石川寿樹、宮城敦子、山口雅利、川合真紀
    • Organizer
      第37回日本植物細胞分子生物学会(京都)大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネのアルミニウム耐性におけるスフィンゴ脂質cis/trans異性体の機能差異2019

    • Author(s)
      佐藤正弥、石川寿樹、長野稔、山口雅利、川合真紀
    • Organizer
      第37回日本植物細胞分子生物学会(京都)大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シロイヌナズナの種子特異的なスフィンゴ糖脂質は種子サイズの制御に関与する2019

    • Author(s)
      石川寿樹、小野田瑞希、川合真紀
    • Organizer
      第37回日本植物細胞分子生物学会(京都)大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] クチクラ形成を制御する転写因子による種子保存性の改変2019

    • Author(s)
      大島良美、鳴海貴子、金子康子、石川寿樹、川合真紀、高木優、光田展隆
    • Organizer
      第37回日本植物細胞分子生物学会(京都)大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物の環境適応においてスフィンゴ脂質の分子進化が果たした意義2019

    • Author(s)
      石川寿樹、川合真紀
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 細胞膜を介した植物免疫制御機構の解析2019

    • Author(s)
      長野稔、宇川智水、伴野文彦、石川寿樹、山口雅利、深尾陽一朗、川合真紀
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シロイヌナズナにおけるスフィンゴ脂質糖鎖異常変異体の表現型解析2019

    • Author(s)
      工藤大和、宮城敦子、山口雅利、川合真紀、石川寿樹
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物に特徴的な2つのセラミド不飽和結合の機能解析2019

    • Author(s)
      藤田祐樹、石川寿樹、宮城敦子、山口雅利、川合真紀
    • Organizer
      第32回植物脂質シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] シロイヌナズナにおけるスフィンゴ脂質糖鎖異常変異体の機能解析2019

    • Author(s)
      工藤大和、宮城敦子、山口雅利、川合真紀、石川寿樹
    • Organizer
      第32回植物脂質シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] グルコシルセラミドの長鎖塩基Δ8 cis型不飽和結合はイネのアルミニウム 耐性に寄与する2019

    • Author(s)
      佐藤正弥、石川寿樹、長野稔、川合真紀
    • Organizer
      第32回植物脂質シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] A seed-specific glycosyl head of sphingolipids is associated with regulation of seed size in Arabidopsis2019

    • Author(s)
      Toshiki Ishikawa, Maki Kawai-Yamada
    • Organizer
      8th Asian-Oceanian Symposium on Plant Lipids
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 8 cis-unsaturated glucosylceramides contribute to aluminum tolerance in rice2019

    • Author(s)
      Masaya Sato, 1Toshiki Ishikawa, 2Minoru Nagano, Atsuko Miyagi, Yamaguchi Masatoshi, Maki Kawai-Yamada
    • Organizer
      8th Asian-Oceanian Symposium on Plant Lipids
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物の細胞膜動態におけるスフィンゴ脂質の役割2019

    • Author(s)
      Minoru Nagano, Yohann Boutte, Adiilah Mamode-Cassim, Laetitia Fouillen, Maki Kawai-Yamada, Sebastien Mongrand
    • Organizer
      第60回日本植物生理学会年会 名古屋
    • Related Report
      2018 Annual Research Report
  • [Presentation] The Role of Sphingolipids in the Dynamics of Plasma Membrane in Plants2018

    • Author(s)
      Minoru Nagano, Yohann Boutte, Adiilah Mamode-Cassim, Maki Kawai-Yamada, sebastien Mongrand
    • Organizer
      The 23ed International Symposium on Plant Lipids
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sphingolipid cis/trans unsaturation increases aluminum tolerance in rice2018

    • Author(s)
      Masaya Sato, Toshiki Ishikawa, Maki Kawai-Yamada
    • Organizer
      The 23ed International Symposium on Plant Lipids
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The Evolutionary Journey of Plant-Unique Long-Chain Base Unsaturation2018

    • Author(s)
      Toshiki Ishikawa, Maki Kawai-Yamada
    • Organizer
      The 23ed International Symposium on Plant Lipids
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物におけるスフィンゴ脂質糖鎖構造の多様性形成機構と生物学的意義2018

    • Author(s)
      石川寿樹、川合真紀
    • Organizer
      第36回日本植物細胞分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物に特徴的な2つのセラミド不飽和結合の機能比較2018

    • Author(s)
      藤田祐樹、石川寿樹、山口雅利、川合真紀
    • Organizer
      第36回日本植物細胞分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] コケ植物におけるスフィンゴ脂質Δ8不飽和化酵素の同定と機能解析2018

    • Author(s)
      小川洋佑、石川寿樹、山口雅利、川合真紀
    • Organizer
      第36回日本植物細胞分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] スフィンゴ脂質長鎖塩基Δ8不飽和化改変イネの作出と表現型解析2018

    • Author(s)
      佐藤正弥、石川寿樹、山口雅利、川合真紀
    • Organizer
      第36回日本植物細胞分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] スフィンゴ脂質による植物細胞膜ダイナミクスの制御2018

    • Author(s)
      長野稔、Yohann Boutte;、Adiilah Mamode-Cassim、Laetitia Fouillen、川合真紀、Sebastien Mongrand
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物固有なスフィンゴ脂質糖鎖を形成する糖転移酵素の同定と機能解析2018

    • Author(s)
      石川寿樹、川合真紀
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物型スフィンゴ脂質糖鎖の構造多様性と機能2018

    • Author(s)
      石川寿樹、川合真紀
    • Organizer
      第31回植物脂質シンポジウム
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2024-01-30  

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