| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
The stiffness of extracellular matrix such as collagen regulates the cell differentiation. In this study, we investigated the involvement of the vinculin-SORBS3/1 axis. We found that both SORBS3 and SORBS1 regulate the nuclear localization of YAP in a manner dependent on the stiffness in mesenchymal stem cells. In addition, the results also showed the involvement of SORBS1 in stiffness-dependent regulation into adipocyte differentiation. We also identified a second vinculin-binding site in SORBS3 and demonstrated its importance using biochemical analysis.
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