| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Outline of Final Research Achievements |
Yeast TORC2 localizes to the sterol-rich plasma membrane lipid microdomains (MCT, membrane compartment containing TORC2). We investigated the effect of edelfosine, a drug that disrupts or destabilizes lipid microdomain’s structure, on the activation of TORC2-Ypk1/2 signaling. We found that edelfosine inhibited the activation of TORC2 signaling. On the other hand, edelfosine induced phosphorylation of Mpk1. We examined the effect of Cdc42 deficiency on the activation of TORC2 signaling, and found that activation of TORC2-Ypk1/2 signaling is suppressed in Cdc42-deficient strains.
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