| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2020: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Outline of Final Research Achievements |
This study aimed at establishing a molecular basis for artificial construction of an efficient CO2 influx pathway in plants. First, we co-expressed a chimeric protein fused with cyanobacterial bicarbonate transporter and a protease in plants, and succeeded in accumulating the bicarbonate transporter as an authentic protein in chloroplasts. In addition, by constructing multiple bicarbonate transporter chimeric genes fused with protein A or GFP, two types of bicarbonate transporters could be simultaneously accumulated in chloroplasts at high levels. In addition, we identified proteins that interact with GLK1 protein and analyzed the regulatory mechanisms that may affect the CO2 uptake capacity of bicarbonate transporters.
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