| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
In this research project, we have performed a knockout of orf352, a candidate of CMS-causative gene for WA-type and RT102-type CMS rice, using a TALEN with a mitochondria-targeting signal. As a result, we have obtained some transformants whose orf352 are disrupted. These plants, however, did not show seed fertility. Sequence analysis around the target site of mitoTALEN in orf352 and pollen phenotype observation have revealed that disruption of orf352 partially restores pollen development. These results suggest that multiple genes in the mitochondrial genome may be involved in the induction of male sterility in rice.
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